Recombinase-Mediated Cassette Exchange (RMCE): Traditional Concepts and Current Challenges

Traditional DNA transduction routes used for the modification of cellular genomes are subject to unpredictable alterations, as the cell-intrinsic repair machinery may affect both the integrity of the transgene and the recipient locus. These problems are overcome by recombinase-mediated cassette exch...

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Bibliographic Details
Published in:	Journal of molecular biology Vol. 407; no. 2; pp. 193 - 221
Main Authors:	Turan, Soeren, Galla, Melanie, Ernst, Ellen, Qiao, Junhua, Voelkel, Christine, Schiedlmeier, Bernhard, Zehe, Christoph, Bode, Juergen
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 25.03.2011
Subjects:	Animals DNA DNA Nucleotidyltransferases DNA Nucleotidyltransferases - genetics equipment maintenance and repair Flp-RMCE Gene Targeting Gene Transfer Techniques Genetic Engineering Genetic Engineering - methods genetics integrase mechanism loci methods Mice mutagenesis mutants recombinase-mediated cassette exchange stem cells tag and exchange targeted integration toxicity Transgenes trapping yeasts recombinase-mediated cassette exchange CPP heteromeric RTs RT RV LE/RE pFARs FBE FRT integrase mechanism HR ES cells FACS DSB NLS tag and exchange targeted integration hygtk GOI dRMCE RMDI loxP iPS Flp-RMCE SSR heterospecific RTs ZFNs RMCE
ISSN:	0022-2836, 1089-8638, 1089-8638
Online Access:	Get full text
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