Potential impact of a microarray-based nucleic acid assay for rapid detection of Gram-negative bacteria and resistance markers in positive blood cultures

We evaluated the Verigene Gram-negative blood culture (BC-GN) test, a microarray that detects Gram-negative bacteria and several resistance genes. A total of 102 positive blood cultures were tested, and the BC-GN test correctly identified 97.9% of the isolates within its panel. Resistance genes (CTX...

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Vydáno v:Journal of clinical microbiology Ročník 52; číslo 4; s. 1242
Hlavní autoři: Mancini, Nicasio, Infurnari, Laura, Ghidoli, Nadia, Valzano, Grazia, Clementi, Nicola, Burioni, Roberto, Clementi, Massimo
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 01.04.2014
Témata:
Bacteremia - diagnosis
Drug Resistance, Bacterial
Gram-Negative Bacteria - drug effects
Gram-Negative Bacteria - genetics
Gram-Negative Bacteria - isolation & purification
Gram-Negative Bacterial Infections - diagnosis
Humans
Microarray Analysis - methods
Molecular Diagnostic Techniques - methods
Pilot Projects
Predictive Value of Tests
Sensitivity and Specificity
ISSN:1098-660X, 1098-660X
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Abstract We evaluated the Verigene Gram-negative blood culture (BC-GN) test, a microarray that detects Gram-negative bacteria and several resistance genes. A total of 102 positive blood cultures were tested, and the BC-GN test correctly identified 97.9% of the isolates within its panel. Resistance genes (CTX-M, KPC, VIM, and OXA genes) were detected in 29.8% of the isolates, with positive predictive values of 95.8% (95% confidence interval [CI], 87.7% to 98.9%) in Enterobacteriaceae and 100% (95% CI, 75.9% to 100%) in Pseudomonas aeruginosa and negative predictive values of 100% (95% CI, 93.9% to 100%) and 78.6% (95% CI, 51.0% to 93.6%), respectively.
AbstractList We evaluated the Verigene Gram-negative blood culture (BC-GN) test, a microarray that detects Gram-negative bacteria and several resistance genes. A total of 102 positive blood cultures were tested, and the BC-GN test correctly identified 97.9% of the isolates within its panel. Resistance genes (CTX-M, KPC, VIM, and OXA genes) were detected in 29.8% of the isolates, with positive predictive values of 95.8% (95% confidence interval [CI], 87.7% to 98.9%) in Enterobacteriaceae and 100% (95% CI, 75.9% to 100%) in Pseudomonas aeruginosa and negative predictive values of 100% (95% CI, 93.9% to 100%) and 78.6% (95% CI, 51.0% to 93.6%), respectively.
We evaluated the Verigene Gram-negative blood culture (BC-GN) test, a microarray that detects Gram-negative bacteria and several resistance genes. A total of 102 positive blood cultures were tested, and the BC-GN test correctly identified 97.9% of the isolates within its panel. Resistance genes (CTX-M, KPC, VIM, and OXA genes) were detected in 29.8% of the isolates, with positive predictive values of 95.8% (95% confidence interval [CI], 87.7% to 98.9%) in Enterobacteriaceae and 100% (95% CI, 75.9% to 100%) in Pseudomonas aeruginosa and negative predictive values of 100% (95% CI, 93.9% to 100%) and 78.6% (95% CI, 51.0% to 93.6%), respectively.We evaluated the Verigene Gram-negative blood culture (BC-GN) test, a microarray that detects Gram-negative bacteria and several resistance genes. A total of 102 positive blood cultures were tested, and the BC-GN test correctly identified 97.9% of the isolates within its panel. Resistance genes (CTX-M, KPC, VIM, and OXA genes) were detected in 29.8% of the isolates, with positive predictive values of 95.8% (95% confidence interval [CI], 87.7% to 98.9%) in Enterobacteriaceae and 100% (95% CI, 75.9% to 100%) in Pseudomonas aeruginosa and negative predictive values of 100% (95% CI, 93.9% to 100%) and 78.6% (95% CI, 51.0% to 93.6%), respectively.
Author Infurnari, Laura
Valzano, Grazia
Clementi, Nicola
Mancini, Nicasio
Clementi, Massimo
Burioni, Roberto
Ghidoli, Nadia
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Snippet We evaluated the Verigene Gram-negative blood culture (BC-GN) test, a microarray that detects Gram-negative bacteria and several resistance genes. A total of...
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SubjectTerms Bacteremia - diagnosis
Drug Resistance, Bacterial
Gram-Negative Bacteria - drug effects
Gram-Negative Bacteria - genetics
Gram-Negative Bacteria - isolation & purification
Gram-Negative Bacterial Infections - diagnosis
Humans
Microarray Analysis - methods
Molecular Diagnostic Techniques - methods
Pilot Projects
Predictive Value of Tests
Sensitivity and Specificity
Title Potential impact of a microarray-based nucleic acid assay for rapid detection of Gram-negative bacteria and resistance markers in positive blood cultures
URI https://www.ncbi.nlm.nih.gov/pubmed/24478405
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