Potential impact of a microarray-based nucleic acid assay for rapid detection of Gram-negative bacteria and resistance markers in positive blood cultures

We evaluated the Verigene Gram-negative blood culture (BC-GN) test, a microarray that detects Gram-negative bacteria and several resistance genes. A total of 102 positive blood cultures were tested, and the BC-GN test correctly identified 97.9% of the isolates within its panel. Resistance genes (CTX...

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Veröffentlicht in:Journal of clinical microbiology Jg. 52; H. 4; S. 1242
Hauptverfasser: Mancini, Nicasio, Infurnari, Laura, Ghidoli, Nadia, Valzano, Grazia, Clementi, Nicola, Burioni, Roberto, Clementi, Massimo
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 01.04.2014
Schlagworte:
Bacteremia - diagnosis
Drug Resistance, Bacterial
Gram-Negative Bacteria - drug effects
Gram-Negative Bacteria - genetics
Gram-Negative Bacteria - isolation & purification
Gram-Negative Bacterial Infections - diagnosis
Humans
Microarray Analysis - methods
Molecular Diagnostic Techniques - methods
Pilot Projects
Predictive Value of Tests
Sensitivity and Specificity
ISSN:1098-660X, 1098-660X
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Zusammenfassung:We evaluated the Verigene Gram-negative blood culture (BC-GN) test, a microarray that detects Gram-negative bacteria and several resistance genes. A total of 102 positive blood cultures were tested, and the BC-GN test correctly identified 97.9% of the isolates within its panel. Resistance genes (CTX-M, KPC, VIM, and OXA genes) were detected in 29.8% of the isolates, with positive predictive values of 95.8% (95% confidence interval [CI], 87.7% to 98.9%) in Enterobacteriaceae and 100% (95% CI, 75.9% to 100%) in Pseudomonas aeruginosa and negative predictive values of 100% (95% CI, 93.9% to 100%) and 78.6% (95% CI, 51.0% to 93.6%), respectively.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1098-660X
1098-660X
DOI:10.1128/JCM.00142-14