Development of a Real-Time PCR Assay for the Detection of Donkey (Equus asinus) Meat in Meat Mixtures Treated under Different Processing Conditions

In this study, a donkey-specific primer pair and probe were designed from mitochondrial cytochrome b gene for the detection of raw donkey meat and different processed meat mixtures. The PCR product size for donkey DNA was 99 bp, and primer specificity was verified using 20 animal species. The limit...

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Veröffentlicht in:	Foods Jg. 9; H. 2; S. 130
Hauptverfasser:	Kim, Mi-Ju, Suh, Seung-Man, Kim, Sung-Yeon, Qin, Pei, Kim, Hong-Rae, Kim, Hae-Yeong
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	Switzerland MDPI 26.01.2020 MDPI AG
Schlagworte:	asses autoclaving beef cytochrome b detection limit DNA donkey Equus asinus food adulteration food fraud genes meat products mitochondria oligodeoxyribonucleotides processed meat quantitative polymerase chain reaction real-time pcr donkey meat products real-time PCR food adulteration food fraud cytochrome b
ISSN:	2304-8158, 2304-8158
Online-Zugang:	Volltext
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