Patients with Irritable Bowel Syndrome Exhibit Aberrant Expression of Endogenous Retroviruses and SETDB1

Irritable bowel syndrome (IBS) is a common disease, whose etiopathogenesis is poorly understood. Human endogenous retroviruses (HERVs) originate from ancient infections of germinal cells and represent 8% of our DNA. Most HERVs have become defective due to the accumulated mutations; some can, however...

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Vydané v:Cells (Basel, Switzerland) Ročník 14; číslo 3; s. 196
Hlavní autori: Tovo, Pier-Angelo, Ribaldone, Davide Giuseppe, Caviglia, Gian Paolo, Calvi, Cristina, Montanari, Paola, Tizzani, Marco, Pitoni, Demis, Frara, Simone, Tribocco, Elisa, Gambarino, Stefano, Guariglia, Marta, Galliano, Ilaria, Bergallo, Massimiliano
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Switzerland MDPI AG 29.01.2025
MDPI
Predmet:
Abdomen
Adult
Antigens
Blood & organ donations
Case-Control Studies
Celiac disease
DNA methylation
Endogenous Retroviruses - genetics
Endogenous Retroviruses - metabolism
Enzymes
Epigenetics
Female
Gastrointestinal diseases
Gene regulation
Genes
Histone-Lysine N-Methyltransferase - genetics
Histone-Lysine N-Methyltransferase - metabolism
human endogenous retroviruses
Humans
Immune system
Inflammation
Inflammatory bowel disease
Inflammatory diseases
Intestine
Irritable bowel syndrome
Irritable Bowel Syndrome - genetics
Irritable Bowel Syndrome - virology
Male
Medical innovations
Microbiota
Middle Aged
Motility
pathogenesis
Peripheral blood
Proteins
SETDB1
Syncytin
TRIM28
Tripartite Motif-Containing Protein 28 - genetics
Tripartite Motif-Containing Protein 28 - metabolism
United States > US
pathogenesis
SETDB1
TRIM28
human endogenous retroviruses
irritable bowel syndrome
ISSN:2073-4409, 2073-4409
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Shrnutí:Irritable bowel syndrome (IBS) is a common disease, whose etiopathogenesis is poorly understood. Human endogenous retroviruses (HERVs) originate from ancient infections of germinal cells and represent 8% of our DNA. Most HERVs have become defective due to the accumulated mutations; some can, however, still be activated, and their altered expressions have been associated with a number of chronic inflammatory and immune-mediated disorders, including gastrointestinal diseases. Retroviral transcription is modulated by TRIM28 and SETDB1, which also participate in the regulation of epigenetic mechanisms and in shaping the immune system. Expressions of HERVs and TRIM28/SETDB1 have not been investigated in patients affected by IBS. Using a PCR real-time Taqman amplification assay, we explored the RNA levels of HERV-H-pol, HERV-K-pol, and HERV-W-pol; syncytin 1 (SYN1), SYN2, and HERV-W-env; and TRIM28 and SETDB1 in the peripheral blood of 37 IBS patients and healthy controls (HCs) of similar age. The transcript levels were higher in IBS patients than in HCs for all HERVs except for HERV-W-pol, with significant p-values for HERV-H-pol, HERV-K-pol, and SYN1 and borderline p-values for SYN2 and HERV-W-env. The RNA levels of SETDB1 were significantly enhanced in IBS patients, while those of TRIM28 were in the normal range. Patients with severe disease had significant upregulation of SETDB1 compared to those with mild or moderate symptoms. These findings suggest that overexpression of HERVs and SETDB1 may contribute to the development of IBS and open the way to innovative therapeutic strategies.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
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These authors contributed equally to this work.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells14030196