High membrane permeability for melatonin
The pineal gland, an endocrine organ in the brain, synthesizes and secretes the circulating night hormone melatonin throughout the night. The literature states that this hormone is secreted by simple diffusion across the pinealocyte plasma membrane, but a direct quantitative measurement of membrane...
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| Vydáno v: | The Journal of general physiology Ročník 147; číslo 1; s. 63 |
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| Médium: | Journal Article |
| Jazyk: | angličtina |
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United States
01.01.2016
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| ISSN: | 1540-7748, 1540-7748 |
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| Abstract | The pineal gland, an endocrine organ in the brain, synthesizes and secretes the circulating night hormone melatonin throughout the night. The literature states that this hormone is secreted by simple diffusion across the pinealocyte plasma membrane, but a direct quantitative measurement of membrane permeability has not been made. Experiments were designed to compare the cell membrane permeability to three indoleamines: melatonin and its precursors N-acetylserotonin (NAS) and serotonin (5-HT). The three experimental approaches were (1) to measure the concentration of effluxing indoleamines amperometrically in the bath while cells were being dialyzed internally by a patch pipette, (2) to measure the rise of intracellular indoleamine fluorescence as the compound was perfused in the bath, and (3) to measure the rate of quenching of intracellular fura-2 dye fluorescence as indoleamines were perfused in the bath. These measures showed that permeabilities of melatonin and NAS are high (both are uncharged molecules), whereas that for 5-HT (mostly charged) is much lower. Comparisons were made with predictions of solubility-diffusion theory and compounds of known permeability, and a diffusion model was made to simulate all of the measurements. In short, extracellular melatonin equilibrates with the cytoplasm in 3.5 s, has a membrane permeability of ∼1.7 µm/s, and could not be retained in secretory vesicles. Thus, it and NAS will be "secreted" from pineal cells by membrane diffusion. Circumstances are suggested when 5-HT and possibly catecholamines may also appear in the extracellular space passively by membrane diffusion. |
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| AbstractList | The pineal gland, an endocrine organ in the brain, synthesizes and secretes the circulating night hormone melatonin throughout the night. The literature states that this hormone is secreted by simple diffusion across the pinealocyte plasma membrane, but a direct quantitative measurement of membrane permeability has not been made. Experiments were designed to compare the cell membrane permeability to three indoleamines: melatonin and its precursors N-acetylserotonin (NAS) and serotonin (5-HT). The three experimental approaches were (1) to measure the concentration of effluxing indoleamines amperometrically in the bath while cells were being dialyzed internally by a patch pipette, (2) to measure the rise of intracellular indoleamine fluorescence as the compound was perfused in the bath, and (3) to measure the rate of quenching of intracellular fura-2 dye fluorescence as indoleamines were perfused in the bath. These measures showed that permeabilities of melatonin and NAS are high (both are uncharged molecules), whereas that for 5-HT (mostly charged) is much lower. Comparisons were made with predictions of solubility-diffusion theory and compounds of known permeability, and a diffusion model was made to simulate all of the measurements. In short, extracellular melatonin equilibrates with the cytoplasm in 3.5 s, has a membrane permeability of ∼1.7 µm/s, and could not be retained in secretory vesicles. Thus, it and NAS will be "secreted" from pineal cells by membrane diffusion. Circumstances are suggested when 5-HT and possibly catecholamines may also appear in the extracellular space passively by membrane diffusion.The pineal gland, an endocrine organ in the brain, synthesizes and secretes the circulating night hormone melatonin throughout the night. The literature states that this hormone is secreted by simple diffusion across the pinealocyte plasma membrane, but a direct quantitative measurement of membrane permeability has not been made. Experiments were designed to compare the cell membrane permeability to three indoleamines: melatonin and its precursors N-acetylserotonin (NAS) and serotonin (5-HT). The three experimental approaches were (1) to measure the concentration of effluxing indoleamines amperometrically in the bath while cells were being dialyzed internally by a patch pipette, (2) to measure the rise of intracellular indoleamine fluorescence as the compound was perfused in the bath, and (3) to measure the rate of quenching of intracellular fura-2 dye fluorescence as indoleamines were perfused in the bath. These measures showed that permeabilities of melatonin and NAS are high (both are uncharged molecules), whereas that for 5-HT (mostly charged) is much lower. Comparisons were made with predictions of solubility-diffusion theory and compounds of known permeability, and a diffusion model was made to simulate all of the measurements. In short, extracellular melatonin equilibrates with the cytoplasm in 3.5 s, has a membrane permeability of ∼1.7 µm/s, and could not be retained in secretory vesicles. Thus, it and NAS will be "secreted" from pineal cells by membrane diffusion. Circumstances are suggested when 5-HT and possibly catecholamines may also appear in the extracellular space passively by membrane diffusion. The pineal gland, an endocrine organ in the brain, synthesizes and secretes the circulating night hormone melatonin throughout the night. The literature states that this hormone is secreted by simple diffusion across the pinealocyte plasma membrane, but a direct quantitative measurement of membrane permeability has not been made. Experiments were designed to compare the cell membrane permeability to three indoleamines: melatonin and its precursors N-acetylserotonin (NAS) and serotonin (5-HT). The three experimental approaches were (1) to measure the concentration of effluxing indoleamines amperometrically in the bath while cells were being dialyzed internally by a patch pipette, (2) to measure the rise of intracellular indoleamine fluorescence as the compound was perfused in the bath, and (3) to measure the rate of quenching of intracellular fura-2 dye fluorescence as indoleamines were perfused in the bath. These measures showed that permeabilities of melatonin and NAS are high (both are uncharged molecules), whereas that for 5-HT (mostly charged) is much lower. Comparisons were made with predictions of solubility-diffusion theory and compounds of known permeability, and a diffusion model was made to simulate all of the measurements. In short, extracellular melatonin equilibrates with the cytoplasm in 3.5 s, has a membrane permeability of ∼1.7 µm/s, and could not be retained in secretory vesicles. Thus, it and NAS will be "secreted" from pineal cells by membrane diffusion. Circumstances are suggested when 5-HT and possibly catecholamines may also appear in the extracellular space passively by membrane diffusion. |
| Author | Koh, Duk-Su Yu, Haijie Dickson, Eamonn J Jung, Seung-Ryoung Hille, Bertil |
| Author_xml | – sequence: 1 givenname: Haijie surname: Yu fullname: Yu, Haijie organization: Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195 – sequence: 2 givenname: Eamonn J surname: Dickson fullname: Dickson, Eamonn J organization: Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195 – sequence: 3 givenname: Seung-Ryoung surname: Jung fullname: Jung, Seung-Ryoung organization: Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195 – sequence: 4 givenname: Duk-Su surname: Koh fullname: Koh, Duk-Su organization: Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195 Department of Physics, POSTECH, Pohang, Kyungbuk 790-784, Republic of Korea – sequence: 5 givenname: Bertil surname: Hille fullname: Hille, Bertil email: hille@uw.edu organization: Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195 hille@uw.edu |
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| References | 25712017 - J Gen Physiol. 2015 Mar;145(3):225-51 7659162 - Nature. 1995 Sep 7;377(6544):62-5 1737762 - J Biol Chem. 1992 Feb 15;267(5):3066-73 18945893 - J Neurosci. 2008 Oct 22;28(43):10852-63 7381427 - J Gen Physiol. 1980 Apr;75(4):427-36 1993739 - J Cell Biol. 1991 Feb;112(4):719-25 19606092 - Clin Pharmacol Ther. 2009 Oct;86(4):378-82 300786 - J Gen Physiol. 1977 Apr;69(4):497-515 3735402 - J Membr Biol. 1986;90(3):207-17 9863668 - Br J Pharmacol. 1998 Nov;125(6):1361-7 16143961 - Cell Biochem Funct. 2006 Nov-Dec;24(6):499-505 9283707 - Br J Pharmacol. 1997 Aug;121(8):1707-15 16078 - J Gen Physiol. 1977 Apr;69(4):475-96 956767 - J Gen Physiol. 1976 Aug;68(2):127-35 24203711 - Nucleic Acids Res. 2014 Jan;42(Database issue):D1091-7 19171770 - J Gen Physiol. 2009 Feb;133(2):171-88 17005040 - J Circadian Rhythms. 2006 Sep 27;4:12 8750345 - J Pineal Res. 1995 Oct;19(3):123-6 16182338 - Bioorg Chem. 2005 Dec;33(6):415-25 9482792 - J Neurosci. 1998 Mar 15;18(6):2056-62 9037083 - Proc Natl Acad Sci U S A. 1997 Feb 18;94(4):1506-11 1936532 - Mol Cell Endocrinol. 1991 Aug;79(1-3):C153-8 12773631 - Pharmacol Rev. 2003 Jun;55(2):325-95 7807375 - J Pineal Res. 1994 May;16(4):198-201 25504572 - J Physiol. 2015 Feb 15;593(4):887-904 15180337 - Pharm Res. 2004 May;21(5):799-802 5673304 - J Gen Physiol. 1968 Sep;52(3):495-508 10379582 - J Neurosci Methods. 1999 Apr 1;88(1):83-91 2866468 - Neuroscience. 1985 Jul;15(3):891-902 23630337 - J Gen Physiol. 2013 May;141(5):521-35 |
| References_xml | – reference: 25712017 - J Gen Physiol. 2015 Mar;145(3):225-51 – reference: 16182338 - Bioorg Chem. 2005 Dec;33(6):415-25 – reference: 15180337 - Pharm Res. 2004 May;21(5):799-802 – reference: 9863668 - Br J Pharmacol. 1998 Nov;125(6):1361-7 – reference: 2866468 - Neuroscience. 1985 Jul;15(3):891-902 – reference: 1737762 - J Biol Chem. 1992 Feb 15;267(5):3066-73 – reference: 7659162 - Nature. 1995 Sep 7;377(6544):62-5 – reference: 19171770 - J Gen Physiol. 2009 Feb;133(2):171-88 – reference: 7381427 - J Gen Physiol. 1980 Apr;75(4):427-36 – reference: 23630337 - J Gen Physiol. 2013 May;141(5):521-35 – reference: 19606092 - Clin Pharmacol Ther. 2009 Oct;86(4):378-82 – reference: 9037083 - Proc Natl Acad Sci U S A. 1997 Feb 18;94(4):1506-11 – reference: 12773631 - Pharmacol Rev. 2003 Jun;55(2):325-95 – reference: 24203711 - Nucleic Acids Res. 2014 Jan;42(Database issue):D1091-7 – reference: 10379582 - J Neurosci Methods. 1999 Apr 1;88(1):83-91 – reference: 25504572 - J Physiol. 2015 Feb 15;593(4):887-904 – reference: 7807375 - J Pineal Res. 1994 May;16(4):198-201 – reference: 3735402 - J Membr Biol. 1986;90(3):207-17 – reference: 1993739 - J Cell Biol. 1991 Feb;112(4):719-25 – reference: 1936532 - Mol Cell Endocrinol. 1991 Aug;79(1-3):C153-8 – reference: 18945893 - J Neurosci. 2008 Oct 22;28(43):10852-63 – reference: 9283707 - Br J Pharmacol. 1997 Aug;121(8):1707-15 – reference: 300786 - J Gen Physiol. 1977 Apr;69(4):497-515 – reference: 17005040 - J Circadian Rhythms. 2006 Sep 27;4:12 – reference: 16143961 - Cell Biochem Funct. 2006 Nov-Dec;24(6):499-505 – reference: 9482792 - J Neurosci. 1998 Mar 15;18(6):2056-62 – reference: 5673304 - J Gen Physiol. 1968 Sep;52(3):495-508 – reference: 16078 - J Gen Physiol. 1977 Apr;69(4):475-96 – reference: 956767 - J Gen Physiol. 1976 Aug;68(2):127-35 – reference: 8750345 - J Pineal Res. 1995 Oct;19(3):123-6 |
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| SubjectTerms | Cell Line Cell Membrane Permeability - physiology Fluorescence Fura-2 - metabolism Humans Melatonin - metabolism Pineal Gland - metabolism Serotonin - analogs & derivatives Serotonin - metabolism |
| Title | High membrane permeability for melatonin |
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