Cell-cycle control of cell polarity in yeast

In many cells, morphogenetic events are coordinated with the cell cycle by cyclin-dependent kinases (CDKs). For example, many mammalian cells display extended morphologies during interphase but round up into more spherical shapes during mitosis (high CDK activity) and constrict a furrow during cytok...

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Veröffentlicht in:The Journal of cell biology Jg. 218; H. 1; S. 171
Hauptverfasser: Moran, Kyle D, Kang, Hui, Araujo, Ana V, Zyla, Trevin R, Saito, Koji, Tsygankov, Denis, Lew, Daniel J
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 07.01.2019
Schlagworte:
Actin Cytoskeleton - metabolism
Actin Cytoskeleton - ultrastructure
cdc42 GTP-Binding Protein, Saccharomyces cerevisiae - genetics
cdc42 GTP-Binding Protein, Saccharomyces cerevisiae - metabolism
Cell Polarity - genetics
Cytokinesis - genetics
Feedback, Physiological
G1 Phase Cell Cycle Checkpoints - genetics
Gene Expression Regulation, Fungal
MAP Kinase Kinase Kinases - genetics
MAP Kinase Kinase Kinases - metabolism
Mitosis - genetics
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae - ultrastructure
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Signal Transduction
Time Factors
ISSN:1540-8140, 1540-8140
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Zusammenfassung:In many cells, morphogenetic events are coordinated with the cell cycle by cyclin-dependent kinases (CDKs). For example, many mammalian cells display extended morphologies during interphase but round up into more spherical shapes during mitosis (high CDK activity) and constrict a furrow during cytokinesis (low CDK activity). In the budding yeast , bud formation reproducibly initiates near the G1/S transition and requires activation of CDKs at a point called "start" in G1. Previous work suggested that CDKs acted by controlling the ability of cells to polarize Cdc42, a conserved Rho-family GTPase that regulates cell polarity and the actin cytoskeleton in many systems. However, we report that yeast daughter cells can polarize Cdc42 before CDK activation at start. This polarization operates via a positive feedback loop mediated by the Cdc42 effector Ste20. We further identify a major and novel locus of CDK action downstream of Cdc42 polarization, affecting the ability of several other Cdc42 effectors to localize to the polarity site.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1540-8140
1540-8140
DOI:10.1083/jcb.201806196