The MTT Assay: Utility, Limitations, Pitfalls, and Interpretation in Bulk and Single-Cell Analysis

The MTT assay for cellular metabolic activity is almost ubiquitous to studies of cell toxicity; however, it is commonly applied and interpreted erroneously. We investigated the applicability and limitations of the MTT assay in representing treatment toxicity, cell viability, and metabolic activity....

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Bibliographic Details
Published in:	International journal of molecular sciences Vol. 22; no. 23; p. 12827
Main Authors:	Ghasemi, Mahshid, Turnbull, Tyron, Sebastian, Sonia, Kempson, Ivan
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 26.11.2021 MDPI
Subjects:	Cell Count Cell Survival Culture Media - pharmacology Cytotoxicity Enzymes Gold - chemistry Humans Male Metabolism Metal Nanoparticles - administration & dosage Metal Nanoparticles - chemistry Polyethylene glycol Prostatic Neoplasms - drug therapy Prostatic Neoplasms - metabolism Prostatic Neoplasms - pathology Reagents Secretome Single-Cell Analysis - methods Tumor Cells, Cultured how to gold nanoparticles PC-3 cells interpret cell metabolism image cytometry MTT assay cytotoxicity viability assay
ISSN:	1422-0067, 1661-6596, 1422-0067
Online Access:	Get full text
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