Staphylococci in high resolution: Capturing diversity within the human nasal microbiota

Staphylococci include both nasal commensals and opportunistic pathogens, globally responsible for a large proportion of infection-related deaths, especially in S. aureus carriers. To understand staphylococcal temporal dynamics within the nasal microbiota, we employed Staphylococcus-targeted sequenci...

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Published in:	Cell reports (Cambridge) Vol. 44; no. 6; p. 115854
Main Authors:	Ingham, Anna Cäcilia, Ng, Duncan Y.K., Iversen, Søren, Liu, Cindy M., Dinh, Khoa Manh, Holtfreter, Silva, Edslev, Sofie Marie, Johannesen, Thor Bech, Rendboe, Amalie Katrine, Christiansen, Mette Theilgaard, Ng, Kim Lee, Skov, Robert, Samietz, Stefanie, Radke, Dörte, Weiss, Stefan, Völker, Uwe, Bröker, Barbara M., Erikstrup, Lise Tornvig, Erikstrup, Christian, Price, Lance B., Andersen, Paal Skytt, Stegger, Marc
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 24.06.2025 Elsevier
Subjects:	Adult antagonist carriage community state type CP: Microbiology Denmark Female Germany Humans Male microbial abundance microbiome Microbiota Middle Aged nasal microbiota Nose - microbiology Staphylococcal Infections - microbiology staphylococci Staphylococcus - classification Staphylococcus - genetics Staphylococcus - isolation & purification Staphylococcus aureus Staphylococcus aureus - genetics Staphylococcus aureus - isolation & purification Staphylococcus epidermidis Staphylococcus epidermidis - genetics Staphylococcus epidermidis - isolation & purification Denmark Germany Staphylococcus epidermidis carriage community state type nasal microbiota CP: Microbiology staphylococci microbial abundance microbiome antagonist Staphylococcus aureus
ISSN:	2211-1247, 2211-1247
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Summary:	Staphylococci include both nasal commensals and opportunistic pathogens, globally responsible for a large proportion of infection-related deaths, especially in S. aureus carriers. To understand staphylococcal temporal dynamics within the nasal microbiota, we employed Staphylococcus-targeted sequencing in two cohorts from Denmark and Germany. We identified two major staphylococcal community state types (sCSTs)—one dominated by S. aureus and one dominated by S. epidermidis—and eight subgroups defined by co-colonizing coagulase-negative staphylococci. The distribution of sCSTs was similar between the two cohorts. Predominance of either S. aureus or S. epidermidis was highly persistent over time, whereas co-colonizing staphylococcal species were transient with varying stability among the sCST subgroups. Detection of S. aureus by culture was positively associated with absolute abundance by qPCR. S. aureus domination was diminished when Dolosigranulum and Corynebacterium co-occurred. Our findings could inform efforts to reduce S. aureus nasal colonization and infection. [Display omitted] •The human nose harbors two major staphylococcal community state types (sCSTs)•S. aureus and S. epidermidis dominate the two sCSTs alongside various co-colonizers•Predominance of either S. aureus or S. epidermidis is highly persistent over time•sCSTs are comparable across two cohorts from Denmark and Germany Nasal staphylococci cluster into two stable community state types, dominated by either S. aureus or S. epidermidis, in a Danish and a German cohort. Each type included distinct subclusters defined by co-colonizing staphylococci. S. aureus was reduced when co-occurring with Dolosigranulum and Corynebacterium, highlighting microbial interactions relevant to infection prevention.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 AUTHOR CONTRIBUTIONS Conceptualization, A.C.I., S.M.E., M.S., K.M.D., L.T.E., C.E., B.M.B., U.V., S. H., D.Y.K.N., L.B.P., R.S., P.S.A., and S.I.; data curation, A.C.I., K.M.D., L.T. E., C.E., C.M.L., D.Y.K.N., T.B.J., S.H., S.W., D.R., and S.I.; formal analysis, A.C.I. and D.Y.K.N.; funding acquisition, M.S., K.M.D., L.T.E., C.E., L.B.P., B.M.B., and P.S.A.; investigation, M.S., K.M.D., L.T.E., C.E., S.H., D.R., S.S., S.W., M.T.C., A.K.R., and S.I.; methodology, M.S., A.C.I., D.Y.K.N., M.T.C., T.B.J., and S.I.; project administration, A.C.I., S.M.E., D.Y.K.N., P.S.A., and M.S.; resources, M.S., K.M.D., L.T.E., C.E., and P.S.A.; software, K.L.N.; supervision, A.C.I., M.S., D.Y.K.N., and P.S.A.; validation, A.C.I. and D.Y.K.N.; visualization, A.C.I. and D.Y.K.N.; writing – original draft, A.C.I. and D.Y.K.N.; writing – review & editing, A.C.I., S.M.E., M.S., K.M.D., L.T.E., C.E., L.B.P., C.M.L., B.M.B., U.V., S.H., D.Y.K.N., P.S.A., S.I., and R.S. All authors read and approved the final manuscript.
ISSN:	2211-1247 2211-1247
DOI:	10.1016/j.celrep.2025.115854