Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method

This protocol is a practical guide to the N-methyl-D-glucamine (NMDG) protective recovery method of brain slice preparation. Numerous recent studies have validated the utility of this method for enhancing neuronal preservation and overall brain slice viability. The implementation of this technique b...

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Bibliographic Details
Published in:	Journal of visualized experiments no. 132
Main Authors:	Ting, Jonathan T., Lee, Brian R., Chong, Peter, Soler-Llavina, Gilberto, Cobbs, Charles, Koch, Christof, Zeng, Hongkui, Lein, Ed
Format:	Journal Article
Language:	English
Published:	United States MyJove Corporation 26.02.2018
Subjects:	Animals Biophysical Phenomena brain Brain - pathology Brain - surgery cerebrospinal fluid electrophysiology Female Glutamate Decarboxylase - genetics Glutamate Decarboxylase - metabolism juveniles Male Mice Mice, Transgenic neurons Neuroscience slicing transgenic animals viability young adults
ISSN:	1940-087X, 1940-087X
Online Access:	Get full text
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