Analysis and characterization of antiviral drug-resistant cytomegalovirus isolates from solid organ transplant recipients

The development of cytomegalovirus (CMV) disease and subsequent emergence of drug-resistant strains was examined in a large group of solid organ transplant recipients; drug-resistant CMV was detected in a total of 30 transplant recipients (20 lung, 5 kidney, 4 heart, and 1 liver). Drug resistance wa...

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Published in:The Journal of infectious diseases Vol. 186; no. 6; p. 760
Main Authors: Lurain, Nell S, Bhorade, Sangeeta M, Pursell, Kenneth J, Avery, Robin K, Yeldandi, Vijay V, Isada, Carlos M, Robert, Emmanuel S, Kohn, Debra J, Arens, Max Q, Garrity, Edward R, Taege, Alan J, Mullen, Martin G, Todd, Kathleen M, Bremer, James W, Yen-Lieberman, Belinda
Format: Journal Article
Language:English
Published: United States 15.09.2002
Subjects:
Antiviral Agents - pharmacology
Antiviral Agents - therapeutic use
Cohort Studies
Cytomegalovirus - drug effects
Cytomegalovirus - genetics
Cytomegalovirus - isolation & purification
Cytomegalovirus Infections - drug therapy
Cytomegalovirus Infections - virology
DNA, Viral - analysis
Drug Resistance, Viral - genetics
Genes, Viral - genetics
Genetic Variation
Humans
Mutation
Organ Transplantation
Phenotype
Polymerase Chain Reaction
ISSN:0022-1899
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Summary:The development of cytomegalovirus (CMV) disease and subsequent emergence of drug-resistant strains was examined in a large group of solid organ transplant recipients; drug-resistant CMV was detected in a total of 30 transplant recipients (20 lung, 5 kidney, 4 heart, and 1 liver). Drug resistance was confirmed both phenotypically and genotypically. The sequences of drug-resistant CMV strains from the same patient differed from drug-susceptible baseline sequences only at single sites previously confirmed to confer drug resistance. At least 1 isolate from each patient had a mutation in the UL97 phosphotransferase coding sequence. Mutations in the DNA polymerase gene were found in 6 of 38 sequenced strains. Lung transplant recipients had the highest incidence of drug-resistant virus: of the 30 patients, 28 were CMV-seronegative transplant recipients of CMV-seropositive organs, which strongly supports the premise that drug resistance is most prevalent in that transplant population.
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ISSN:0022-1899
DOI:10.1086/342844