A Simultaneous Extraction/Derivatization Strategy for Quantitation of Vitamin D in Dried Blood Spots Using LC–MS/MS: Application to Biomarker Study in Subjects Tested for SARS-CoV-2
Vitamin D plays a critical role in bone development and maintenance, and in other physiological functions. The quantitation of endogenous levels of individual vitamin D and its metabolites is crucial for assessing several disease state conditions. With cases of severe acute respiratory syndrome coro...
Gespeichert in:
| Veröffentlicht in: | International journal of molecular sciences Jg. 24; H. 6; S. 5489 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Journal Article |
| Sprache: | Englisch |
| Veröffentlicht: |
Switzerland
MDPI AG
13.03.2023
MDPI |
| Schlagworte: | |
| ISSN: | 1422-0067, 1661-6596, 1422-0067 |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| Abstract | Vitamin D plays a critical role in bone development and maintenance, and in other physiological functions. The quantitation of endogenous levels of individual vitamin D and its metabolites is crucial for assessing several disease state conditions. With cases of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) leading to the coronavirus disease 2019 (COVID-19) pandemic, there are several studies that have associated lower levels of serum vitamin D with severity of infection in COVID-19 patients. In this context, we have developed and validated a robust LC–MS/MS method for simultaneous quantitation of vitamin D and its metabolites in human dried blood spot (DBS) obtained from participants tested for COVID-19. The chromatographic separation for vitamin D and metabolites was performed using an ACE Excel C18 PFP column protected with a C18 guard column (Phenomenex, Torrance, CA, USA). The mobile phase consisted of formic acid in water (0.1% v/v) as mobile phase A and formic acid in methanol (0.1% v/v) as mobile phase B, operated at a flow rate of 0.5 mL/min. Analysis was performed utilizing the LC–MS/MS technique. The method was sensitive with a limit of quantification of 0.78 ng/mL for all analytes, and had a large dynamic range (200 ng/mL) with a total run time of 11 min. The inter- and intraday accuracy and precision values met the acceptance criteria per the US Food and Drug Administration guidelines. Blood concentrations of 25(OH)D3, vitamin D3, 25(OH)D2, and vitamin D2 over a range of 2–195.6, 0.5–121.5, 0.6–54.9, and 0.5–23.9 ng/mL, respectively, were quantified in 909 DBS samples. In summary, our developed LC−MS/MS method may be used for quantification of vitamin D and its metabolites in DBS, and may be applied to investigations of the emerging role of these compounds in various physiological processes. |
|---|---|
| AbstractList | Vitamin D plays a critical role in bone development and maintenance, and in other physiological functions. The quantitation of endogenous levels of individual vitamin D and its metabolites is crucial for assessing several disease state conditions. With cases of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) leading to the coronavirus disease 2019 (COVID-19) pandemic, there are several studies that have associated lower levels of serum vitamin D with severity of infection in COVID-19 patients. In this context, we have developed and validated a robust LC-MS/MS method for simultaneous quantitation of vitamin D and its metabolites in human dried blood spot (DBS) obtained from participants tested for COVID-19. The chromatographic separation for vitamin D and metabolites was performed using an ACE Excel C18 PFP column protected with a C18 guard column (Phenomenex, Torrance, CA, USA). The mobile phase consisted of formic acid in water (0.1% v/v) as mobile phase A and formic acid in methanol (0.1% v/v) as mobile phase B, operated at a flow rate of 0.5 mL/min. Analysis was performed utilizing the LC-MS/MS technique. The method was sensitive with a limit of quantification of 0.78 ng/mL for all analytes, and had a large dynamic range (200 ng/mL) with a total run time of 11 min. The inter- and intraday accuracy and precision values met the acceptance criteria per the US Food and Drug Administration guidelines. Blood concentrations of 25(OH)D3, vitamin D3, 25(OH)D2, and vitamin D2 over a range of 2-195.6, 0.5-121.5, 0.6-54.9, and 0.5-23.9 ng/mL, respectively, were quantified in 909 DBS samples. In summary, our developed LC-MS/MS method may be used for quantification of vitamin D and its metabolites in DBS, and may be applied to investigations of the emerging role of these compounds in various physiological processes.Vitamin D plays a critical role in bone development and maintenance, and in other physiological functions. The quantitation of endogenous levels of individual vitamin D and its metabolites is crucial for assessing several disease state conditions. With cases of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) leading to the coronavirus disease 2019 (COVID-19) pandemic, there are several studies that have associated lower levels of serum vitamin D with severity of infection in COVID-19 patients. In this context, we have developed and validated a robust LC-MS/MS method for simultaneous quantitation of vitamin D and its metabolites in human dried blood spot (DBS) obtained from participants tested for COVID-19. The chromatographic separation for vitamin D and metabolites was performed using an ACE Excel C18 PFP column protected with a C18 guard column (Phenomenex, Torrance, CA, USA). The mobile phase consisted of formic acid in water (0.1% v/v) as mobile phase A and formic acid in methanol (0.1% v/v) as mobile phase B, operated at a flow rate of 0.5 mL/min. Analysis was performed utilizing the LC-MS/MS technique. The method was sensitive with a limit of quantification of 0.78 ng/mL for all analytes, and had a large dynamic range (200 ng/mL) with a total run time of 11 min. The inter- and intraday accuracy and precision values met the acceptance criteria per the US Food and Drug Administration guidelines. Blood concentrations of 25(OH)D3, vitamin D3, 25(OH)D2, and vitamin D2 over a range of 2-195.6, 0.5-121.5, 0.6-54.9, and 0.5-23.9 ng/mL, respectively, were quantified in 909 DBS samples. In summary, our developed LC-MS/MS method may be used for quantification of vitamin D and its metabolites in DBS, and may be applied to investigations of the emerging role of these compounds in various physiological processes. Vitamin D plays a critical role in bone development and maintenance, and in other physiological functions. The quantitation of endogenous levels of individual vitamin D and its metabolites is crucial for assessing several disease state conditions. With cases of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) leading to the coronavirus disease 2019 (COVID-19) pandemic, there are several studies that have associated lower levels of serum vitamin D with severity of infection in COVID-19 patients. In this context, we have developed and validated a robust LC-MS/MS method for simultaneous quantitation of vitamin D and its metabolites in human dried blood spot (DBS) obtained from participants tested for COVID-19. The chromatographic separation for vitamin D and metabolites was performed using an ACE Excel C18 PFP column protected with a C18 guard column (Phenomenex, Torrance, CA, USA). The mobile phase consisted of formic acid in water (0.1% v/v) as mobile phase A and formic acid in methanol (0.1% v/v) as mobile phase B, operated at a flow rate of 0.5 mL/min. Analysis was performed utilizing the LC-MS/MS technique. The method was sensitive with a limit of quantification of 0.78 ng/mL for all analytes, and had a large dynamic range (200 ng/mL) with a total run time of 11 min. The inter- and intraday accuracy and precision values met the acceptance criteria per the US Food and Drug Administration guidelines. Blood concentrations of 25(OH)D3, vitamin D3, 25(OH)D2, and vitamin D2 over a range of 2-195.6, 0.5-121.5, 0.6-54.9, and 0.5-23.9 ng/mL, respectively, were quantified in 909 DBS samples. In summary, our developed LC−MS/MS method may be used for quantification of vitamin D and its metabolites in DBS, and may be applied to investigations of the emerging role of these compounds in various physiological processes. Vitamin D plays a critical role in bone development and maintenance, and in other physiological functions. The quantitation of endogenous levels of individual vitamin D and its metabolites is crucial for assessing several disease state conditions. With cases of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) leading to the coronavirus disease 2019 (COVID-19) pandemic, there are several studies that have associated lower levels of serum vitamin D with severity of infection in COVID-19 patients. In this context, we have developed and validated a robust LC-MS/MS method for simultaneous quantitation of vitamin D and its metabolites in human dried blood spot (DBS) obtained from participants tested for COVID-19. The chromatographic separation for vitamin D and metabolites was performed using an ACE Excel C18 PFP column protected with a C18 guard column (Phenomenex, Torrance, CA, USA). The mobile phase consisted of formic acid in water (0.1% / ) as mobile phase A and formic acid in methanol (0.1% / ) as mobile phase B, operated at a flow rate of 0.5 mL/min. Analysis was performed utilizing the LC-MS/MS technique. The method was sensitive with a limit of quantification of 0.78 ng/mL for all analytes, and had a large dynamic range (200 ng/mL) with a total run time of 11 min. The inter- and intraday accuracy and precision values met the acceptance criteria per the US Food and Drug Administration guidelines. Blood concentrations of 25(OH)D3, vitamin D3, 25(OH)D2, and vitamin D2 over a range of 2-195.6, 0.5-121.5, 0.6-54.9, and 0.5-23.9 ng/mL, respectively, were quantified in 909 DBS samples. In summary, our developed LC-MS/MS method may be used for quantification of vitamin D and its metabolites in DBS, and may be applied to investigations of the emerging role of these compounds in various physiological processes. |
| Audience | Academic |
| Author | Chhonker, Yashpal S. Ahmed, Nusrat Johnston, Christine M. Murry, Daryl J. Barnabas, Ruanne V. |
| AuthorAffiliation | 5 Fred and Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198, USA 3 Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA 02114, USA 2 Department of Medicine, University of Washington, Seattle, WA 98101, USA 4 Department of Medicine, Harvard Medical School, Boston, MA 02115, USA 1 Clinical Pharmacology Laboratory, Department of Pharmacy Practice and Science, University of Nebraska Medical Center, Omaha, NE 68198, USA |
| AuthorAffiliation_xml | – name: 3 Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA 02114, USA – name: 4 Department of Medicine, Harvard Medical School, Boston, MA 02115, USA – name: 1 Clinical Pharmacology Laboratory, Department of Pharmacy Practice and Science, University of Nebraska Medical Center, Omaha, NE 68198, USA – name: 5 Fred and Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198, USA – name: 2 Department of Medicine, University of Washington, Seattle, WA 98101, USA |
| Author_xml | – sequence: 1 givenname: Yashpal S. orcidid: 0000-0001-6455-5388 surname: Chhonker fullname: Chhonker, Yashpal S. – sequence: 2 givenname: Nusrat surname: Ahmed fullname: Ahmed, Nusrat – sequence: 3 givenname: Christine M. surname: Johnston fullname: Johnston, Christine M. – sequence: 4 givenname: Ruanne V. surname: Barnabas fullname: Barnabas, Ruanne V. – sequence: 5 givenname: Daryl J. orcidid: 0000-0002-4169-5027 surname: Murry fullname: Murry, Daryl J. |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/36982565$$D View this record in MEDLINE/PubMed |
| BookMark | eNptks1u1DAUhS1URNuBHWtkiQ0L0nH8l4QNmk7LjzQVgrTdWh7HGTwk9hA7Vacr3oGH4X14EhxmKNOqipQ4ud899j05h2DPOqsBeJ6iI0IKNDbL1mOKOKN58QgcpBTjBCGe7e2s98Gh90uEMMGseAL2CS9yzDg7AL8msDRt3wRptes9PL0OnVTBODs-0Z25ksHcyOEVlrEQ9GINa9fBz720wYRNxdXwMq5bY-EJHG6d0RU8bpyrYLlywcMLb-wCzqa_f_w8K8dn5Rs4Wa0aozb9wcFj41rZfdNd3Kav1oNK2c-XWsXmc-1D1Bu2LSdfymTqLhP8FDyuZeP1s-1zBC7enZ5PPySzT-8_TiezRNEchUSzCnGii4IRxXNZ8Sy6JJlmKM-qjGJFWEVqRvk81Wqe8VrpNLpGuKaSakrJCLzd6K76easrpW20oRGrzsTzroWTRtytWPNVLNyVSBFidHB8BF5tFTr3vY-ziNZ4pZtm47jAWYEZwnnGIvryHrp0fWfjfAOVcsY5If-phWy0MLZ2wy8bRMUkY2mB_3IjcPQAFa9Kt0bFCNUmfr_T8GJ30tsR_2UlAq83gOqc952ub5EUiSGKYjeKEcf3cLUNTDyIaR5u-gMX9uLX |
| CitedBy_id | crossref_primary_10_1016_j_aca_2023_341964 crossref_primary_10_3390_ijms241210316 crossref_primary_10_1016_j_aca_2025_344223 crossref_primary_10_3390_separations11070197 crossref_primary_10_1016_j_saa_2025_126936 |
| Cites_doi | 10.1007/s00216-019-02183-5 10.7326/M20-6519 10.1016/j.jpba.2020.113529 10.14735/amko201599 10.1007/s11914-012-0098-z 10.1007/s00011-014-0755-z 10.1016/j.jsbmb.2012.09.019 10.1016/j.ejmech.2022.114175 10.1016/j.jchromb.2020.122394 10.1016/j.chroma.2019.460418 10.33549/physiolres.934492 10.1002/rmv.2032 10.1016/j.jchromb.2014.05.006 10.1016/j.bonr.2018.05.003 10.1002/bmc.4102 10.1016/S1674-8301(11)60048-1 10.1016/j.chemphyslip.2020.104932 10.3389/fendo.2019.00910 10.1016/j.eclinm.2021.100773 10.1016/j.jsbmb.2020.105719 10.3390/ph15030305 10.1016/j.jcot.2019.07.004 10.3390/nu12113361 10.1016/j.jchromb.2015.04.011 10.1016/j.coph.2015.04.001 10.1515/cclm-2019-0397 10.3390/nu13113932 10.1016/j.lfs.2020.117808 10.2174/18715281113129990046 10.1016/j.cca.2018.02.024 10.1101/2021.09.22.21263977 |
| ContentType | Journal Article |
| Copyright | COPYRIGHT 2023 MDPI AG 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2023 by the authors. 2023 |
| Copyright_xml | – notice: COPYRIGHT 2023 MDPI AG – notice: 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: 2023 by the authors. 2023 |
| DBID | AAYXX CITATION CGR CUY CVF ECM EIF NPM 3V. 7X7 7XB 88E 8FI 8FJ 8FK 8G5 ABUWG AFKRA AZQEC BENPR CCPQU COVID DWQXO FYUFA GHDGH GNUQQ GUQSH K9. M0S M1P M2O MBDVC PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQQKQ PQUKI PRINS Q9U 7X8 5PM |
| DOI | 10.3390/ijms24065489 |
| DatabaseName | CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed ProQuest Central (Corporate) Health & Medical Collection (ProQuest) ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) ProQuest Hospital Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) Research Library (Alumni) ProQuest Central (Alumni) ProQuest Central UK/Ireland ProQuest Central Essentials ProQuest Central Database Suite (ProQuest) ProQuest One Community College Coronavirus Research Database ProQuest Central Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student Research Library Prep (ProQuest) ProQuest Health & Medical Complete (Alumni) ProQuest Health & Medical Collection Medical Database Research Library (ProQuest) Research Library (Corporate) ProQuest Central Premium ProQuest One Academic Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic (retired) ProQuest One Academic UKI Edition ProQuest Central China ProQuest Central Basic MEDLINE - Academic PubMed Central (Full Participant titles) |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Publicly Available Content Database Research Library Prep ProQuest Central Student ProQuest One Academic Middle East (New) ProQuest Central Essentials ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest One Health & Nursing Research Library (Alumni Edition) ProQuest Central China ProQuest Central ProQuest Health & Medical Research Collection Health Research Premium Collection Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Health & Medical Research Collection ProQuest Research Library ProQuest Central (New) ProQuest Medical Library (Alumni) ProQuest Central Basic ProQuest One Academic Eastern Edition Coronavirus Research Database ProQuest Hospital Collection Health Research Premium Collection (Alumni) ProQuest Hospital Collection (Alumni) ProQuest Health & Medical Complete ProQuest Medical Library ProQuest One Academic UKI Edition ProQuest One Academic ProQuest One Academic (New) ProQuest Central (Alumni) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic MEDLINE Publicly Available Content Database CrossRef |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: PIMPY name: Publicly Available Content Database url: http://search.proquest.com/publiccontent sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Biology |
| EISSN | 1422-0067 |
| ExternalDocumentID | PMC10054002 A751926566 36982565 10_3390_ijms24065489 |
| Genre | Journal Article |
| GeographicLocations | Massachusetts |
| GeographicLocations_xml | – name: Massachusetts |
| GrantInformation_xml | – fundername: NCI NIH HHS grantid: P30 CA036727 – fundername: Bill and Melinda Gates Foundation grantid: INV-017062 and INV-01620 – fundername: National Cancer Institute grantid: P30 CA036727 – fundername: Bill and Melinda Gates Foundation grantid: INV-017062; INV-01620 |
| GroupedDBID | --- 29J 2WC 53G 5GY 5VS 7X7 88E 8FE 8FG 8FH 8FI 8FJ 8G5 A8Z AADQD AAFWJ AAHBH AAYXX ABDBF ABUWG ACGFO ACIHN ACIWK ACPRK ACUHS ADBBV AEAQA AENEX AFFHD AFKRA AFZYC ALMA_UNASSIGNED_HOLDINGS AOIJS AZQEC BAWUL BCNDV BENPR BPHCQ BVXVI CCPQU CITATION CS3 D1I DIK DU5 DWQXO E3Z EBD EBS EJD ESX F5P FRP FYUFA GNUQQ GUQSH GX1 HH5 HMCUK HYE IAO IHR ITC KQ8 LK8 M1P M2O M48 MODMG O5R O5S OK1 OVT P2P PHGZM PHGZT PIMPY PJZUB PPXIY PQQKQ PROAC PSQYO RNS RPM TR2 TUS UKHRP ~8M 3V. ABJCF ALIPV BBNVY BHPHI CGR CUY CVF ECM EIF GROUPED_DOAJ HCIFZ KB. M7P M~E NPM PDBOC 7XB 8FK COVID ESTFP K9. MBDVC PKEHL PQEST PQUKI PRINS Q9U 7X8 PUEGO 5PM |
| ID | FETCH-LOGICAL-c480t-e5d063e9953c68ad67489a5e5087d742c35d3f546b1ecb76fce114236e4a4e443 |
| IEDL.DBID | 7X7 |
| ISICitedReferencesCount | 6 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000956178200001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 1422-0067 1661-6596 |
| IngestDate | Tue Nov 04 02:07:21 EST 2025 Thu Oct 02 08:22:57 EDT 2025 Tue Oct 07 07:41:38 EDT 2025 Sat Nov 29 13:06:16 EST 2025 Sat Nov 29 10:08:58 EST 2025 Wed Feb 19 02:24:23 EST 2025 Tue Nov 18 19:44:47 EST 2025 Sat Nov 29 07:15:06 EST 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 6 |
| Keywords | biomarker LC–MS/MS SARS-CoV-2(+) vitamin D |
| Language | English |
| License | Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c480t-e5d063e9953c68ad67489a5e5087d742c35d3f546b1ecb76fce114236e4a4e443 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 These authors contributed equally to this work. |
| ORCID | 0000-0001-6455-5388 0000-0002-4169-5027 |
| OpenAccessLink | https://www.proquest.com/docview/2791656633?pq-origsite=%requestingapplication% |
| PMID | 36982565 |
| PQID | 2791656633 |
| PQPubID | 2032341 |
| ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_10054002 proquest_miscellaneous_2792502875 proquest_journals_2791656633 gale_infotracmisc_A751926566 gale_infotracacademiconefile_A751926566 pubmed_primary_36982565 crossref_primary_10_3390_ijms24065489 crossref_citationtrail_10_3390_ijms24065489 |
| PublicationCentury | 2000 |
| PublicationDate | 20230313 |
| PublicationDateYYYYMMDD | 2023-03-13 |
| PublicationDate_xml | – month: 3 year: 2023 text: 20230313 day: 13 |
| PublicationDecade | 2020 |
| PublicationPlace | Switzerland |
| PublicationPlace_xml | – name: Switzerland – name: Basel |
| PublicationTitle | International journal of molecular sciences |
| PublicationTitleAlternate | Int J Mol Sci |
| PublicationYear | 2023 |
| Publisher | MDPI AG MDPI |
| Publisher_xml | – name: MDPI AG – name: MDPI |
| References | Lee (ref_34) 2016; 46 Glenville (ref_22) 2016; 164 Teegarden (ref_32) 2015; 991 Alghareeb (ref_17) 2021; 52 ref_36 Galior (ref_28) 2018; 8 Pisoschi (ref_13) 2022; 232 Suda (ref_4) 2015; 22 Ertugrul (ref_20) 2020; 230 ref_19 ref_18 Stanley (ref_33) 2014; 961 Lieben (ref_5) 2013; 136 ref_16 Xu (ref_27) 2019; 1608 Jensen (ref_21) 2018; 481 Pawar (ref_26) 2020; 10 Bouillon (ref_30) 2020; 10 Paces (ref_11) 2020; 69 Shokri (ref_10) 2019; 29 Luca (ref_15) 2021; 13 Young (ref_6) 2018; 13 Zakaria (ref_24) 2019; 58 Inserra (ref_14) 2020; 254 Sieglinde (ref_23) 2020; 1158 Rola (ref_31) 2020; 190 Meg (ref_8) 2014; 63 Charoenngam (ref_2) 2019; 10 Barnabas (ref_37) 2021; 174 Zhang (ref_25) 2019; 411 Ahadi (ref_29) 2011; 25 Johnston (ref_38) 2021; 33 (ref_7) 2015; 28 Gunville (ref_9) 2013; 12 Nair (ref_1) 2012; 3 Thakare (ref_35) 2018; 32 Bikle (ref_3) 2012; 10 Gomez (ref_12) 2020; 202 |
| References_xml | – volume: 10 start-page: 1 year: 2020 ident: ref_26 article-title: A LC–MS method for 25-hydroxy-vitamin D3 measurements from dried blood spots for an epidemiological survey in India publication-title: Sci. Rep. – volume: 411 start-page: 8073 year: 2019 ident: ref_25 article-title: Improving long-term stability of retinol in dried blood spots and quantification of its levels via a novel LC-MS/MS method publication-title: Anal. Bioanal. Chem. doi: 10.1007/s00216-019-02183-5 – volume: 13 start-page: 43 year: 2018 ident: ref_6 article-title: Influence of Vitamin D on Cancer Risk and Treatment: Why the Variability? publication-title: Trends Cancer Res. – volume: 174 start-page: 344 year: 2021 ident: ref_37 article-title: Hydroxychloroquine as Postexposure Prophylaxis to Prevent Severe Acute Respiratory Syndrome Coronavirus 2 Infection: A Randomized Trial publication-title: Ann. Intern. Med. doi: 10.7326/M20-6519 – volume: 190 start-page: 113529 year: 2020 ident: ref_31 article-title: Improved sample preparation method for fast LC-MS/MS analysis of vitamin D metabolites in serum publication-title: J. Pharm. Biomed. Anal. doi: 10.1016/j.jpba.2020.113529 – volume: 28 start-page: 99 year: 2015 ident: ref_7 article-title: Vitamin D During Cancer Treatment publication-title: Klin. Onkol. doi: 10.14735/amko201599 – volume: 10 start-page: 151 year: 2012 ident: ref_3 article-title: Vitamin D and Bone publication-title: Curr. Osteoporos. Rep. doi: 10.1007/s11914-012-0098-z – volume: 63 start-page: 803 year: 2014 ident: ref_8 article-title: Inflammation and vitamin D: The infection connection publication-title: Inflamm. Res. doi: 10.1007/s00011-014-0755-z – volume: 136 start-page: 102 year: 2013 ident: ref_5 article-title: The delicate balance between vitamin D, calcium and bone homeostasis: Lessons learned from intestinal- and osteocyte-specific VDR null mice publication-title: J. Steroid Biochem. Mol. Biol. doi: 10.1016/j.jsbmb.2012.09.019 – volume: 232 start-page: 114175 year: 2022 ident: ref_13 article-title: Antioxidant, anti-inflammatory and immunomodulatory roles of vitamins in COVID-19 therapy publication-title: Eur. J. Med. Chem. doi: 10.1016/j.ejmech.2022.114175 – volume: 1158 start-page: 122394 year: 2020 ident: ref_23 article-title: Simultaneous Determination of 24,25- and 25,26-Dihydroxyvitamin D3 in Serum Samples with Liquid-Chromatography Mass Spectrometry—a Useful Tool for the Assessment of Vitamin D Metabolism publication-title: J. Chromatogr. B doi: 10.1016/j.jchromb.2020.122394 – volume: 1608 start-page: 460418 year: 2019 ident: ref_27 article-title: Effects of chromatographic conditions and mass spectrometric parameters on the ionization and fragmentation of triterpene saponins of Ilex asprella in liquid chromatography–mass spectrometry analysis publication-title: J. Chromatogr. A doi: 10.1016/j.chroma.2019.460418 – volume: 69 start-page: 379 year: 2020 ident: ref_11 article-title: COVID-19 and the immune system publication-title: Physiol. Res. doi: 10.33549/physiolres.934492 – volume: 29 start-page: e2032 year: 2019 ident: ref_10 article-title: The interplay between vitamin D and viral infections publication-title: Rev. Med. Virol. doi: 10.1002/rmv.2032 – volume: 961 start-page: 62 year: 2014 ident: ref_33 article-title: Development and validation of an LC–MS/MS based method for quantification of 25 hydroxyvitamin D2 and 25 hydroxyvitamin D3 in human serum and plasma publication-title: J. Chromatogr. B Anal. Technol. Biomed. Life Sci. doi: 10.1016/j.jchromb.2014.05.006 – volume: 8 start-page: 268 year: 2018 ident: ref_28 article-title: 10 years of 25-hydroxyvitamin-D testing by LC-MS/MS-trends in vitamin-D deficiency and sufficiency publication-title: Bone Rep. doi: 10.1016/j.bonr.2018.05.003 – volume: 32 start-page: e4102 year: 2018 ident: ref_35 article-title: Simultaneous Lc-Ms/Ms Analysis of Eicosanoids and Related Metabolites in Human Serum, Sputum and Balf publication-title: Biomed. Chromatogr doi: 10.1002/bmc.4102 – volume: 25 start-page: 362 year: 2011 ident: ref_29 article-title: Comparison of liquid-liquid extraction-thin layer chromatography with solid-phase extraction-high-performance thin layer chromatography in detection of urinary morphine publication-title: J. Biomed. Res. doi: 10.1016/S1674-8301(11)60048-1 – volume: 46 start-page: 645 year: 2016 ident: ref_34 article-title: A Rapid and Simple Liquid-Chromatography-Tandem Mass Spectrometry Method for Measuring 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 in Human Serum: Comparison with Two Automated Immunoassays publication-title: Ann. Clin. Lab. Sci. – volume: 230 start-page: 104932 year: 2020 ident: ref_20 article-title: Development and optimization of simultaneous determination of fat soluble vitamins by liquid chromatography tandem mass spectrometry publication-title: Chem. Phys. Lipids doi: 10.1016/j.chemphyslip.2020.104932 – volume: 10 start-page: 910 year: 2020 ident: ref_30 article-title: Vitamin D Binding Protein: A Historic Overview publication-title: Front. Endocrinol. doi: 10.3389/fendo.2019.00910 – volume: 33 start-page: 100773 year: 2021 ident: ref_38 article-title: Hydroxychloroquine with or without Azithromycin for Treatment of Early SARS-CoV-2 Infection among High-Risk Outpatient Adults: A Randomized Clinical Trial publication-title: EClinicalMedicine doi: 10.1016/j.eclinm.2021.100773 – volume: 202 start-page: 105719 year: 2020 ident: ref_12 article-title: Vitamin D Receptor Stimulation to Reduce Acute Respiratory Distress Syndrome (Ards) in Patients with Coronavirus SARS-CoV-2 Infections: Revised Ms Sbmb 2020_166 publication-title: J. Steroid Biochem. Mol. Biol. doi: 10.1016/j.jsbmb.2020.105719 – ident: ref_19 doi: 10.3390/ph15030305 – volume: 3 start-page: 118 year: 2012 ident: ref_1 article-title: Vitamin D: The Sunshine Vitamin publication-title: J. Pharm. Pharm. – volume: 52 start-page: 240 year: 2021 ident: ref_17 article-title: In COVID-19 patients, low 25-hydroxyvitamin D level in serum is associated with longer viral clearance time and higher risk of intensive care unit admission publication-title: Nutr. Food Sci. – volume: 10 start-page: 1082 year: 2019 ident: ref_2 article-title: Vitamin D for skeletal and non-skeletal health: What we should know publication-title: J. Clin. Orthop. Trauma doi: 10.1016/j.jcot.2019.07.004 – ident: ref_16 doi: 10.3390/nu12113361 – volume: 991 start-page: 118 year: 2015 ident: ref_32 article-title: Chromatographic separation of PTAD-derivatized 25-hydroxyvitamin D3 and its C-3 epimer from human serum and murine skin publication-title: J. Chromatogr. B doi: 10.1016/j.jchromb.2015.04.011 – volume: 22 start-page: 87 year: 2015 ident: ref_4 article-title: Physiological functions of vitamin D: What we have learned from global and conditional VDR knockout mouse studies publication-title: Curr. Opin. Pharmacol. doi: 10.1016/j.coph.2015.04.001 – volume: 58 start-page: 817 year: 2019 ident: ref_24 article-title: Candidate reference method for determination of vitamin D from dried blood spot samples publication-title: Clin. Chem. Lab. Med. doi: 10.1515/cclm-2019-0397 – volume: 13 start-page: 3932 year: 2021 ident: ref_15 article-title: Vitamin D Serum Levels in Subjects Tested for SARS-CoV-2: What Are the Differences among Acute, Healed, and Negative COVID-19 Patients? A Multicenter Real-Practice Study publication-title: Nutrients doi: 10.3390/nu13113932 – volume: 254 start-page: 117808 year: 2020 ident: ref_14 article-title: Lungs as target of COVID-19 infection: Protective common molecular mechanisms of vitamin D and melatonin as a new potential synergistic treatment publication-title: Life Sci. doi: 10.1016/j.lfs.2020.117808 – volume: 12 start-page: 239 year: 2013 ident: ref_9 article-title: The Role of Vitamin D in Prevention and Treatment of Infection publication-title: Inflamm. Allergy-Drug Targets doi: 10.2174/18715281113129990046 – volume: 481 start-page: 61 year: 2018 ident: ref_21 article-title: Quantitation of 25-hydroxyvitamin D in dried blood spots by 2D LC-MS/MS without derivatization and correlation with serum in adult and pediatric studies publication-title: Clin. Chim. Acta doi: 10.1016/j.cca.2018.02.024 – ident: ref_18 doi: 10.1101/2021.09.22.21263977 – ident: ref_36 – volume: 164 start-page: 10 year: 2016 ident: ref_22 article-title: Vitamin D Metabolite Profiling Using Liquid Chromatography–Tandem Mass Spectrometry (Lc–Ms/Ms) publication-title: J. Steroid Biochem. Mol. Biol. |
| SSID | ssj0023259 |
| Score | 2.4023569 |
| Snippet | Vitamin D plays a critical role in bone development and maintenance, and in other physiological functions. The quantitation of endogenous levels of individual... |
| SourceID | pubmedcentral proquest gale pubmed crossref |
| SourceType | Open Access Repository Aggregation Database Index Database Enrichment Source |
| StartPage | 5489 |
| SubjectTerms | Alfacalcidol Biomarkers Blood Calcifediol Chromatography Chromatography, Liquid - methods Coronaviruses COVID-19 Diagnosis Disease Formic acid Health aspects Humans Infections Ions Medical research Medicine, Experimental Metabolites Organic acids Pandemics Physiological aspects Physiology Plasma Reproducibility of Results Retention SARS-CoV-2 Severe acute respiratory syndrome Severe acute respiratory syndrome coronavirus 2 Tandem Mass Spectrometry - methods Vitamin D Vitamins |
| Title | A Simultaneous Extraction/Derivatization Strategy for Quantitation of Vitamin D in Dried Blood Spots Using LC–MS/MS: Application to Biomarker Study in Subjects Tested for SARS-CoV-2 |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/36982565 https://www.proquest.com/docview/2791656633 https://www.proquest.com/docview/2792502875 https://pubmed.ncbi.nlm.nih.gov/PMC10054002 |
| Volume | 24 |
| WOSCitedRecordID | wos000956178200001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVPQU databaseName: Health & Medical Collection customDbUrl: eissn: 1422-0067 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0023259 issn: 1422-0067 databaseCode: 7X7 dateStart: 20000301 isFulltext: true titleUrlDefault: https://search.proquest.com/healthcomplete providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Central customDbUrl: eissn: 1422-0067 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0023259 issn: 1422-0067 databaseCode: BENPR dateStart: 20000301 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: Publicly Available Content Database customDbUrl: eissn: 1422-0067 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0023259 issn: 1422-0067 databaseCode: PIMPY dateStart: 20000301 isFulltext: true titleUrlDefault: http://search.proquest.com/publiccontent providerName: ProQuest – providerCode: PRVPQU databaseName: Research Library customDbUrl: eissn: 1422-0067 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0023259 issn: 1422-0067 databaseCode: M2O dateStart: 20000301 isFulltext: true titleUrlDefault: https://search.proquest.com/pqrl providerName: ProQuest |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3LjtMwFLVgBiQ2vB-FYWQkEAsUNYmdh9mgTqcjkGgpk2HUWUVO7IgimpQmRXTHP_Ax_A9fwr1O-ggSbNhEjXzbuvXxfeX4XkKehiLjqcczy5ZKYuomtKSrbUsoVzq-0JkSyjSbCEajcDIR4ybhVja0yrVONIpaFSnmyLtuILBQjM_Yq_kXC7tG4dPVpoXGZbKPbbMR58FkG3Ax1zRLc8AGWb4n_Jr4ziDM704_zUo0ZuCwi5ZJ-lMx71imNmtyxwyd3PjfH3CTXG8cUNqrEXOLXNL5bXK1bkm5ukN-9mg0RZahzHWxLOngW7Wozz50jwGsmMCtD27Spq7tioLbS98vZV415b5pkdFzeD2b5vSY4gXCcUWPkCJPo3lRldQQFejb_q_vP4ZRdxi9pL3tg3RaFRTmM0Pi0IIi0XGFnwIqDnNGJT0zKVrztVHvNLL6xbnl3iUfTgZn_ddW09zBSnloV5b2FHhHWgiPpX4oFTY9EdLT4DAGCuL1lHmKZR73E0enSeBnqcZjv8zXXHLNObtH9vIi1w8ITR3wIpMw4Yq7PAl0IrnCyCuxRag8ITvkxXp947T5K7ABx-cYIiBEQ7yLhg55tpGe1xU__iL3HKESoyLAlZDNeQaYE5bUinsBOMcurniHHLQkYQOn7eE1YOJGgZTxFi0d8mQzjO9EUpxBAMqAAwshr9ch92tsbmbMfAGxvw8jYQu1GwEsK94eyacfTXlxx7jxtvvw3_N6RK65sMWQj-ewA7JXLZb6MbmSfq2m5eLQbERzDQ_J_tFgND6Fu6H7Du7Gb4bji99nS0QF |
| linkProvider | ProQuest |
| linkToHtml | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Lb9NAEF6VFAQX3o9AgUWi4oCs2N71Y5EQCkmrRm2iQEJVTmbtXYsgYofYAXLjP_A_uPJ_-CXM2E4aI8GtBy5WpJ3Yq_W8_c0MIY99EfPI4bFhSiUxdeMb0tamIZQtLVfoWAlVDJvwBgP_5EQMt8iPVS0MwipXOrFQ1CqNMEfesj2BjWJcxl7MPhk4NQq_rq5GaJRscaiXXyBky573uvB-d217f2_cOTCqqQJGxH0zN7SjwCxrIRwWub5UOG1DSEeDp-IpCBQj5igWO9wNLR2FnhtHGutNmau55JpzBvc9R7Y5MnuDbA97_eHbdYjH7GI8mwVWz3Ad4ZZQe8aE2Zp8mGZoPiFEEDUj-Kcp2LCFdZzmhuHbv_K_HdlVcrlysWm7lIlrZEsn18mFcujm8gb52aajCeIoZaLTRUb3vubzsrqj1QVxxBR1WZpKq869SwqOPX21kEleNTSnaUyP4fd0ktAuxcscPHn6EosA6GiW5hktoBj0qPPr2_f-qNUfPaPtU6gAzVMK-5kiNGpOEcq5xLuAEsesWEbHRRK6eOyo_XpkdNJjw75J3pzJqd0ijSRN9B1CIwv85NAPueI2Dz0dSq4wtgxN4StHyCZ5uuKnIKqOAkeMfAwgxkPuCza5r0l219SzsqfJX-ieIGsGqOrwTciqYgP2hE3DgrYH7r-NHNYkOzVKUFFRfXnFoEGlIrPglDub5NF6Gf-JsL-CA5AGXHQI6p0muV3KwnrHzBU--Ouw4tekZE2AjdPrK8nkfdFA3SoCFdO---99PSQXD8b9o-CoNzi8Ry7ZIN6IPrTYDmnk84W-T85Hn_NJNn9QqQFK3p21GP0GcQKcLw |
| linkToPdf | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Lb9NAEF6VFBAX3o9AgUWi4oCs2Ov1Y5EQCkkjojZRIG1VTmbtXYsgYofYAXLjP_BjkPg5_BJmbCeNkeDWAxcr0k7s1Xre_maGkMe-iHnk8NgwpZKYuvENybRpCMWk5QodK6GKYRPecOifnIjRFvm5qoVBWOVKJxaKWqUR5shbzBPYKMa17VZcwSJG3d6L2ScDJ0jhl9bVOI2SRfb18guEb9nzfhfe9S5jvb3DziujmjBgRNw3c0M7Cky0FsKxI9eXCidvCOlo8Fo8BUFjZDvKjh3uhpaOQs-NI421p7arueSacxvue45sg0vOWYNsj_qD0dt1uGezYlSbBRbQcB3hlrB72xZma_JhmqEphXBB1Azin2Zhwy7WMZsbRrB35X8-vqvkcuV603YpK9fIlk6ukwvlMM7lDfKjTccTxFfKRKeLjO59zedl1UerC2KKqeuyZJVWHX2XFBx--nohk7xqdE7TmB7D7-kkoV2Klzl4-PQlFgfQ8SzNM1pANOhB59e374NxazB-RtunEAKapxT2M0XI1JwixHOJdwHljtmyjB4WyeniseP2m7HRSY8NdpMcncmp3SKNJE30HUIjC_zn0A-54oyHng4lVxhzhqbwlSNkkzxd8VYQVUeBo0c-BhD7IScGm5zYJLtr6lnZ6-QvdE-QTQNUgfgmZFXJAXvCZmJB24OwgCG3NclOjRJUV1RfXjFrUKnOLDjl1CZ5tF7GfyIcsOAApAHXHYJ9p0lul3Kx3rHtCh_8eFjxaxKzJsCG6vWVZPK-aKxuFQGMye7-e18PyUWQneCgP9y_Ry4xkHQEJVr2Dmnk84W-T85Hn_NJNn9QaQRK3p21FP0GypWk7w |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+Simultaneous+Extraction%2FDerivatization+Strategy+for+Quantitation+of+Vitamin+D+in+Dried+Blood+Spots+Using+LC%E2%80%93MS%2FMS%3A+Application+to+Biomarker+Study+in+Subjects+Tested+for+SARS-CoV-2&rft.jtitle=International+journal+of+molecular+sciences&rft.au=Chhonker%2C+Yashpal+S&rft.au=Ahmed%2C+Nusrat&rft.au=Johnston%2C+Christine+M&rft.au=Barnabas%2C+Ruanne+V&rft.date=2023-03-13&rft.pub=MDPI+AG&rft.issn=1422-0067&rft.volume=24&rft.issue=6&rft_id=info:doi/10.3390%2Fijms24065489&rft.externalDocID=A751926566 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1422-0067&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1422-0067&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1422-0067&client=summon |