Asymmetrically lesioned mesencephalon in healthy rodents: call for caution

Stereological counting of tyrosine-hydroxylase immunoreactive (TH-IR) neurons in the mesencephalon is a pivotal parameter in assessing the extent of lesioning in animal models of Parkinson's disease. We here show that the number of TH-IR neurons often appears abnormally decreased in healthy—com...

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Veröffentlicht in:Brain research Jg. 1022; H. 1; S. 251 - 253
Hauptverfasser: Pioli, Elsa Y., Dovero, Sandra, Bioulac, Bernard H., Gross, Christian E., Bezard, Erwan
Format: Journal Article
Sprache:Englisch
Veröffentlicht: London Elsevier B.V 01.10.2004
Amsterdam Elsevier
New York, NY
Schlagworte:
Animals
Biological and medical sciences
Cell Count - methods
Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases
Disease Models, Animal
Immunohistochemistry - methods
Male
Medical sciences
Mesencephalon - injuries
Mesencephalon - pathology
Mice
Mice, Inbred C57BL
Neurology
Neurons - metabolism
Neuroprotection
Parkinson Disease - metabolism
Parkinson Disease - pathology
Parkinson's disease
Probability
Rats
Rats, Wistar
Rodentia
Substantia nigra pars compacta
Tyrosine 3-Monooxygenase - metabolism
Tyrosine hydroxylase
J: Disorders of the nervous system
Neuroprotection
Tyrosine hydroxylase
Parkinson's disease
Substantia nigra pars compacta
Animal model
Rat
Central nervous system
Parkinson disease
Tyrosine 3-monooxygenase
Encephalon
Degenerative disease
Nervous system diseases
Enzyme
Rodentia
Midbrain
Cerebral disorder
Vertebrata
Mammalia
Locus niger
Asymmetry
Mouse
Central nervous system disease
Oxidoreductases
Lesion
Extrapyramidal syndrome
ISSN:0006-8993, 1872-6240
Online-Zugang:Volltext
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Beschreibung
Zusammenfassung:Stereological counting of tyrosine-hydroxylase immunoreactive (TH-IR) neurons in the mesencephalon is a pivotal parameter in assessing the extent of lesioning in animal models of Parkinson's disease. We here show that the number of TH-IR neurons often appears abnormally decreased in healthy—commercially available—mice and rats, although both the number of Nissl-stained cells and the striatal dopaminergic innervation are unaffected. This potential bias in assessing extent of neurotoxin-induced lesion and subsequent protection by pharmacological manipulation prompts us to call for caution in setting up experimental designs.
Bibliographie:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0006-8993
1872-6240
DOI:10.1016/j.brainres.2004.07.014