Diversity of toxin genotypes and antimicrobial susceptibility of Clostridium perfringens isolates from feces of infants

This study investigated the most important epidemiological characteristics of strains colonizing the intestine of Jordanian infants. A total of 302 fecal samples were collected from Jordanian infants aged ≤ 1 year from patients hospitalized in the neonatal intensive care unit and from the outpatient...

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Vydané v:Germs (Bucureşti) Ročník 9; číslo 1; s. 28 - 34
Hlavní autori: AL Radaideh, Alaa J, Badran, Eman F, Shehabi, Asem A
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Romania Asociatia pentru Cresterea Vizibilitatii Cercetarii Stiintifice (ACVCS) 01.03.2019
European HIV/AIDS and Infectious Diseases Academy
Asociația pentru Creşterea Vizibilității Cercetării Ştiințifice (ACVCS)
Predmet:
Antibacterial agents
Antimicrobial agents
Antiprotozoan agents
Birth weight
Deoxyribonucleic acid
Diarrhea
DNA
Drug resistance
Epidemiology
Erythromycin
Feces
Genes
Genetic aspects
Genetic research
Genotypes
Identification
Infants
Intensive care
Levofloxacin
Metronidazole
Neonatal intensive care
Newborn infants
Original
Plasmids
Studies
Toxins
Vancomycin
United States > US
toxins genotypes
antimicrobial resistance
infants feces
Clostridium perfringens
ISSN:2248-2997, 2248-2997
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Shrnutí:This study investigated the most important epidemiological characteristics of strains colonizing the intestine of Jordanian infants. A total of 302 fecal samples were collected from Jordanian infants aged ≤ 1 year from patients hospitalized in the neonatal intensive care unit and from the outpatient department. Samples were cultured for detection of and evaluation of their antimicrobial resistance; identification of their potential toxins genes was performed using PCR. Overall the colonization rate was 27.2% (82/302). Infants aged ≤ 6 months showed significantly higher (p<0.004) colonization than older infants. The occurrence rates of isolates carrying potential specific toxin genes were as follows: alpha toxin 95.1% (78/82), beta-2 toxin 69.5% (57/82), beta toxin 14.6% (12/82), and only 2.4% (2/82) epsilon toxin. No isolate carried the iota toxin. Genotype A was the most prevalent among isolates (78.1%). The antimicrobial resistance rates of isolates were 20% to metronidazole and erythromycin, 16.7% to levofloxacin, and 6.7% to vancomycin. This study demonstrates that the majority of isolates from feces of Jordanian infants were classified as type A, few isolates were classified as type C and type D, and all were negative for potential enterotoxin genes causing diarrhea.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:2248-2997
2248-2997
DOI:10.18683/germs.2019.1154