Restriction fragment length polymorphism-based genotyping of Toxoplasma gondii from autopsy-proven cases of acquired immunodeficiency syndrome-associated cerebral toxoplasmosis

Context: Published data on genetic characterization of Toxoplasma gondii (T.gondii) from clinical cases of toxoplasmosis from India is lacking. Aims: The present study was aimed at identifying genetic types of T. gondii in fatal cases of cerebral toxoplasmosis (CT) associated with HIV, from India. S...

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Vydané v:Annals of the Indian Academy of Neurology Ročník 21; číslo 4; s. 250 - 255
Hlavní autori: Vijaykumar, B, Kant, R, Rajendran, C, Lekshmi, Swathi, Keerthana, Sundar, Mahadevan, Anita, Shankar, S, Jayshree, R
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: India Wolters Kluwer India Pvt. Ltd 01.10.2018
Medknow Publications & Media Pvt. Ltd
Medknow Publications & Media Pvt Ltd
Wolters Kluwer Medknow Publications
Predmet:
Acquired immune deficiency syndrome
AIDS
Antigens
Autopsy
Brain research
Cerebral toxoplasmosis
Deoxyribonucleic acid
DNA
Gene amplification
Gene loci
Gene polymorphism
Genetic diversity
Genomes
Genotype & phenotype
Genotyping
HIV
HIV/Acquired Immunodeficiency Syndrome patients
Human immunodeficiency virus
Immunodeficiency
Infections
Medical research
multilocus polymerase chain reaction-restriction fragment length polymorphism genotyping
Original
Parasites
Polymerase chain reaction
Polymorphism
Population genetics
Protozoa
Recombinants
Restriction fragment length polymorphism
Rodents
Studies
Toxoplasma gondii
Toxoplasmosis
United States > US
North America
Europe
China
India
Toxoplasma gondii
Cerebral toxoplasmosis
HIV/Acquired Immunodeficiency Syndrome patients
multilocus polymerase chain reaction-restriction fragment length polymorphism genotyping
ISSN:0972-2327, 1998-3549
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Shrnutí:Context: Published data on genetic characterization of Toxoplasma gondii (T.gondii) from clinical cases of toxoplasmosis from India is lacking. Aims: The present study was aimed at identifying genetic types of T. gondii in fatal cases of cerebral toxoplasmosis (CT) associated with HIV, from India. Settings and Design: Archived tissues of CT were obtained postmortem from 25 acquired immunodeficiency syndrome patients between 2000 and 2014. Subjects and Methods: Direct amplification of eight different loci, namely, SAG1, 5'-3'SAG2, Alt. SAG2, SAG3, BTUB, GRA6, C22-8, and L358 followed by restriction fragment length polymorphism was used to genotype the parasite. Results: The canonical Types I, II, or III were not found in our study. More than 96% of the cases harbored atypical genotypes-likely recombinants of the canonical types; one case closely corresponded to Type II genotype. Conclusions: Thus, a majority of T. gondii causing CT in South India belonged to a noncanonical lineage. These nonarchetypal genotypes differed from the conventional Types I, II, and III and caused devastating severity in patients with CT in the background of HIV. These results are a step further to deciphering the population genetics of this important zoonotic parasitic infection in Indian patients, information that has thus far been lacking.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0972-2327
1998-3549
DOI:10.4103/aian.AIAN_358_17