A New Enterobacter cloacae Bacteriophage EC151 Encodes the Deazaguanine DNA Modification Pathway and Represents a New Genus within the Siphoviridae Family

A novel Enterobacter cloacae phage, EC151, was isolated and characterized. Electron microscopy revealed that EC151 has a siphovirus-like virion morphology. The EC151 nucleotide sequence shows limited similarity to other phage genomes deposited in the NCBI GenBank database. The size of the EC151 geno...

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Bibliographic Details
Published in:Viruses Vol. 13; no. 7; p. 1372
Main Authors: Morozova, Vera, Jdeed, Ghadeer, Kozlova, Yuliya, Babkin, Igor, Tikunov, Artem, Tikunova, Nina
Format: Journal Article
Language:English
Published: Basel MDPI AG 15.07.2021
MDPI
Subjects:
Bacteria
bacteriophages
Biosynthesis
deazaguanine modification pathway
Deoxyribonucleic acid
DNA
Electron microscopy
Enterobacter cloacae
Enterobacter phage
Enzymes
Escherichia
Genes
genetic databases
Genetic distance
Genomes
Genomics
Microscopy
New genera
new genus
Nonagvirus
Nosocomial infections
Nucleotide sequence
Open reading frames
Phages
Phylogeny
preQ0 transporter
Proteins
proteomics
Seuratvirus
Siphoviridae
Synteny
tRNA Ser
virion
Virions
United States > US
Russia
ISSN:1999-4915, 1999-4915
Online Access:Get full text
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Summary:A novel Enterobacter cloacae phage, EC151, was isolated and characterized. Electron microscopy revealed that EC151 has a siphovirus-like virion morphology. The EC151 nucleotide sequence shows limited similarity to other phage genomes deposited in the NCBI GenBank database. The size of the EC151 genome is 60,753 bp and contains 58 putative genes. Thirty-nine of them encode proteins of predicted function, 18 are defined as hypothetical proteins, and one ORF identifies as the tRNA-Ser-GCT-encoding gene. Six ORFs were predicted to be members of the deazaguanine DNA modification pathway, including the preQ0 transporter. Comparative proteomic phylogenetic analysis revealed that phage EC151 represents a distinct branch within a group of sequences containing clades formed by members of the Seuratvirus, Nonagvirus, and Vidquintavirus genera. In addition, the EC151 genome showed gene synteny typical of the Seuratvirus, Nonagvirus, and Nipunavirus phages. The average genetic distances of EC151/Seuratvirus, EC151/Nonagvirus, and EC151/Vidquintavirus are approximately equal to those between the Seuratvirus, Nonagvirus, and Vidquintavirus genera (~0.7 substitutions per site). Therefore, EC151 may represent a novel genus within the Siphoviridae family. The origin of the deazaguanine DNA modification pathway in the EC151 genome can be traced to Escherichia phages from the Seuratvirus genus.
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ISSN:1999-4915
1999-4915
DOI:10.3390/v13071372