Characterization of bluetongue virus ribonucleic acid

An improved purification procedure yielded bluetongue virus free from any single-stranded ribonucleic acid (RNA) component. Double-stranded RNA obtained from purified virus or isolated from infected cells was fractionated into 5 components by means of sucrose gradient sedimentation analysis, and int...

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Published in:Journal of virology Vol. 5; no. 1; p. 1
Main Authors: Verwoerd, D W, Louw, H, Oellermann, R A
Format: Journal Article
Language:English
Published: United States 01.01.1970
Subjects:
Acrylates
Animals
Bluetongue - etiology
Carbon Isotopes
Cell Line
Centrifugation, Density Gradient
Chromatography
Cricetinae
Electrophoresis
Formaldehyde
Gels
Hot Temperature
Indicators and Reagents
Kidney
L Cells (Cell Line)
Molecular Weight
Nucleic Acid Denaturation
Phenols
Phosphorus Isotopes
Reoviridae - analysis
RNA, Viral - analysis
RNA, Viral - isolation & purification
Sheep
Spectrophotometry
Sucrose
Tritium
Uridine
Viruses, Unclassified - isolation & purification
ISSN:0022-538X
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Abstract An improved purification procedure yielded bluetongue virus free from any single-stranded ribonucleic acid (RNA) component. Double-stranded RNA obtained from purified virus or isolated from infected cells was fractionated into 5 components by means of sucrose gradient sedimentation analysis, and into 10 components by electrophoresis on polyacrylamide gels. The size of these components vary from 0.5 x 10(6) to 2.8 x 10(6) daltons, with a total molecular weight estimate of about 1.5 x 10(7) for the viral nucleic acid. The denaturation of the genome and separation of the resulting fragments are also discussed.
AbstractList An improved purification procedure yielded bluetongue virus free from any single-stranded ribonucleic acid (RNA) component. Double-stranded RNA obtained from purified virus or isolated from infected cells was fractionated into 5 components by means of sucrose gradient sedimentation analysis, and into 10 components by electrophoresis on polyacrylamide gels. The size of these components vary from 0.5 x 10(6) to 2.8 x 10(6) daltons, with a total molecular weight estimate of about 1.5 x 10(7) for the viral nucleic acid. The denaturation of the genome and separation of the resulting fragments are also discussed.
An improved purification procedure yielded bluetongue virus free from any single-stranded ribonucleic acid (RNA) component. Double-stranded RNA obtained from purified virus or isolated from infected cells was fractionated into 5 components by means of sucrose gradient sedimentation analysis, and into 10 components by electrophoresis on polyacrylamide gels. The size of these components vary from 0.5 x 10(6) to 2.8 x 10(6) daltons, with a total molecular weight estimate of about 1.5 x 10(7) for the viral nucleic acid. The denaturation of the genome and separation of the resulting fragments are also discussed.An improved purification procedure yielded bluetongue virus free from any single-stranded ribonucleic acid (RNA) component. Double-stranded RNA obtained from purified virus or isolated from infected cells was fractionated into 5 components by means of sucrose gradient sedimentation analysis, and into 10 components by electrophoresis on polyacrylamide gels. The size of these components vary from 0.5 x 10(6) to 2.8 x 10(6) daltons, with a total molecular weight estimate of about 1.5 x 10(7) for the viral nucleic acid. The denaturation of the genome and separation of the resulting fragments are also discussed.
Author Verwoerd, D W
Louw, H
Oellermann, R A
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References 4306588 - Virology. 1969 Jun;38(2):203-12
6034985 - Anal Biochem. 1967 Jul;20(1):114-49
5339944 - Biochem J. 1967 Jan;102(1):251-7
5621469 - J Virol. 1967 Aug;1(4):665-77
5760535 - J Mol Biol. 1968 Aug 28;36(1):107-23
6055334 - J Mol Biol. 1967 Oct 14;29(1):1-17
14498283 - Biochem Biophys Res Commun. 1962 Jun 4;7:486-90
4289760 - Biochem Biophys Res Commun. 1966 May 25;23(4):557-60
5723704 - J Virol. 1968 Oct;2(10):986-91
14290352 - J Mol Biol. 1965 Feb;11:373-90
5242127 - Proc Natl Acad Sci U S A. 1968 Jan;59(1):246-53
5760451 - J Mol Biol. 1968 May 28;34(1):137-47
4966975 - Onderstepoort J Vet Res. 1967 Dec;34(2):317-32
References_xml – reference: 4306588 - Virology. 1969 Jun;38(2):203-12
– reference: 14498283 - Biochem Biophys Res Commun. 1962 Jun 4;7:486-90
– reference: 6055334 - J Mol Biol. 1967 Oct 14;29(1):1-17
– reference: 5760451 - J Mol Biol. 1968 May 28;34(1):137-47
– reference: 5723704 - J Virol. 1968 Oct;2(10):986-91
– reference: 6034985 - Anal Biochem. 1967 Jul;20(1):114-49
– reference: 5339944 - Biochem J. 1967 Jan;102(1):251-7
– reference: 5760535 - J Mol Biol. 1968 Aug 28;36(1):107-23
– reference: 4966975 - Onderstepoort J Vet Res. 1967 Dec;34(2):317-32
– reference: 14290352 - J Mol Biol. 1965 Feb;11:373-90
– reference: 5621469 - J Virol. 1967 Aug;1(4):665-77
– reference: 4289760 - Biochem Biophys Res Commun. 1966 May 25;23(4):557-60
– reference: 5242127 - Proc Natl Acad Sci U S A. 1968 Jan;59(1):246-53
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Snippet An improved purification procedure yielded bluetongue virus free from any single-stranded ribonucleic acid (RNA) component. Double-stranded RNA obtained from...
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SubjectTerms Acrylates
Animals
Bluetongue - etiology
Carbon Isotopes
Cell Line
Centrifugation, Density Gradient
Chromatography
Cricetinae
Electrophoresis
Formaldehyde
Gels
Hot Temperature
Indicators and Reagents
Kidney
L Cells (Cell Line)
Molecular Weight
Nucleic Acid Denaturation
Phenols
Phosphorus Isotopes
Reoviridae - analysis
RNA, Viral - analysis
RNA, Viral - isolation & purification
Sheep
Spectrophotometry
Sucrose
Tritium
Uridine
Viruses, Unclassified - isolation & purification
Title Characterization of bluetongue virus ribonucleic acid
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