Defining the expression pattern of the LGI1 gene in BAC transgenic mice

The LGI1 gene has been implicated in the development of epilepsy and the invasion phenotype of glial cells. Controversy over the specific tissue expression pattern of this gene has stemmed from conflicting reports generated using immunohistochemistry and the polymerase chain reaction. LGI1 is one of...

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Vydáno v:Mammalian genome Ročník 18; číslo 5; s. 328 - 337
Hlavní autoři: Head, Karen, Gong, Shiaoching, Joseph, Sheldon, Wang, Cuidong, Burkhardt, Tania, Rossi, Michael R, LaDuca, Jeffrey, Matsui, Sei-Ichi, Vaughan, Mary, Hicks, David G, Heintz, Nathaniel, Cowell, John K
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States New York : Springer-Verlag 01.05.2007
Springer Nature B.V
Témata:
animal ovaries
Animals
antibodies
bacterial artificial chromosomes
brain
Cell Line
Cercopithecus aethiops
Chromosomes, Artificial, Bacterial - genetics
COS Cells
endometrium
epilepsy
ganglia
gene expression
gene expression regulation
Humans
immunohistochemistry
islets of Langerhans
Mice
Mice, Transgenic
neuroglia
neurons
phenotype
polymerase chain reaction
proteins
Proteins - genetics
renal tubules
reporter genes
sebaceous glands
testes
Transfection
transgenic animals
uncertainty
ISSN:0938-8990, 1432-1777
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Shrnutí:The LGI1 gene has been implicated in the development of epilepsy and the invasion phenotype of glial cells. Controversy over the specific tissue expression pattern of this gene has stemmed from conflicting reports generated using immunohistochemistry and the polymerase chain reaction. LGI1 is one of a four-member family of secreted proteins with high homology and here we demonstrate, using GFP-tagged constructs from the four LGI1family members, that commonly used antibodies against LGI1 cross-react with different family members. With the uncertainty surrounding the use of commercially available antibodies to truly establish the expression pattern of LGI1, we generated transgenic mice carrying the LGI1-containing BAC, RP23-127G7, which had been modified to express the GFP reporter gene under the control of the endogenous regulatory elements required for LGI1 expression. Three founder mice were generated, and immunohistochemistry was used to determine the tissue-specific pattern of expression. In the brain, distinct regions of glial and neuronal cell expression were identified, as well as the choriod plexus, which is largely pia-derived. In addition, strong expression levels were identified in glandular regions of the prostate, individual tubules in the kidney, sympathetic ganglia in the kidney, sebaceous glands in the skin, the islets of Langerhans, the endometrium, and the ovary and testes. All other major organs analyzed were negative. The pattern of reporter gene expression was identical in three individual founder mice, arguing against a position effect altering expression profile due to the integration site of the BAC.
Bibliografie:http://dx.doi.org/10.1007/s00335-007-9024-6
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ISSN:0938-8990
1432-1777
DOI:10.1007/s00335-007-9024-6