qDRIP: a method to quantitatively assess RNA–DNA hybrid formation genome-wide

Abstract R-loops are dynamic, co-transcriptional nucleic acid structures that facilitate physiological processes but can also cause DNA damage in certain contexts. Perturbations of transcription or R-loop resolution are expected to change their genomic distribution. Next-generation sequencing approa...

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Bibliographic Details
Published in:	Nucleic acids research Vol. 48; no. 14; p. e84
Main Authors:	Crossley, Magdalena P, Bocek, Michael J, Hamperl, Stephan, Swigut, Tomek, Cimprich, Karlene A
Format:	Journal Article
Language:	English
Published:	England Oxford University Press 20.08.2020
Subjects:	Animals Cell Line DNA - metabolism Drosophila - cytology Gene Library Genome Half-Life HeLa Cells Humans Immunoprecipitation - methods Methods Online Nucleic Acid Hybridization Polymerase Chain Reaction R-Loop Structures Ribonuclease H RNA - metabolism Sonication Transcription, Genetic
ISSN:	0305-1048, 1362-4962, 1362-4962
Online Access:	Get full text
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