In Silico Evaluation of Putative S100B Interacting Proteins in Healthy and IBD Gut Microbiota

The crosstalk between human gut microbiota and intestinal wall is essential for the organ’s homeostasis and immune tolerance. The gut microbiota plays a role in healthy and pathological conditions mediated by inflammatory processes or by the gut-brain axes, both involving a possible role for S100B p...

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Veröffentlicht in:Cells (Basel, Switzerland) Jg. 9; H. 7; S. 1697
Hauptverfasser: Orsini, Massimiliano, Di Liddo, Rosa, Valeriani, Federica, Mancin, Marzia, D’Incà, Renata, Castagnetti, Andrea, Aceti, Antonio, Parnigotto, Pier Paolo, Romano Spica, Vincenzo, Michetti, Fabrizio
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Switzerland MDPI AG 15.07.2020
MDPI
Schlagworte:
Bacteria
Bioinformatics
Biopsy
Computer Simulation
Digestive system
Gastrointestinal Microbiome
Gastrointestinal tract
Gene Ontology
gut chronic inflammation
Health
Homeostasis
Humans
Immunological tolerance
Inflammatory bowel disease
Inflammatory bowel diseases
Inflammatory Bowel Diseases - microbiology
Intestinal microflora
Intestine
Investigations
microbiome
Microbiota
Mucosa
Ontology
Phylogeny
Pipelines
Protein Domains
Proteins
Proteomes
Proteomics
S100 Calcium Binding Protein beta Subunit - chemistry
S100 Calcium Binding Protein beta Subunit - metabolism
S100B
S100b protein
Taxonomy
Ulcerative colitis
gut chronic inflammation
S100B
microbiome
bioinformatics
ISSN:2073-4409, 2073-4409
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Zusammenfassung:The crosstalk between human gut microbiota and intestinal wall is essential for the organ’s homeostasis and immune tolerance. The gut microbiota plays a role in healthy and pathological conditions mediated by inflammatory processes or by the gut-brain axes, both involving a possible role for S100B protein as a diffusible cytokine present not only in intestinal mucosa but also in faeces. In order to identify target proteins for a putative interaction between S100B and the microbiota proteome, we developed a bioinformatics workflow by integrating the interaction features of known domains with the proteomics data derived from metataxonomic studies of the gut microbiota from healthy and inflammatory bowel disease (IBD) subjects. On the basis of the microbiota composition, proteins putatively interacting with S100B domains were in fact found, both in healthy subjects and IBD patients, in a reduced number in the latter samples, also exhibiting differences in interacting domains occurrence between the two groups. In addition, differences between ulcerative colitis and Crohn disease samples were observed. These results offer the conceptual framework for where to investigate the role of S100B as a candidate signalling molecule in the microbiota/gut communication machinery, on the basis of interactions differently conditioned by healthy or pathological microbiota.
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These authors contributed equally to this work.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells9071697