Confocal Raman microspectroscopy on excised human skin: uncertainties in depth profiling and mathematical correction applied to dermatological drug permeation

Confocal Raman microspectroscopy represents the advantage of giving structural and conformational information on samples without any destructive treatment. Recently, several studies were achieved to study the skin hydration, endogenous and exogenous molecules repartition in the skin using the confoc...

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Vydáno v:Journal of biophotonics Ročník 1; číslo 2; s. 140 - 153
Hlavní autoři: Tfayli, A., Piot, O., Manfait, M.
Médium: Journal Article
Jazyk:angličtina
Vydáno: Berlin WILEY-VCH Verlag 01.05.2008
WILEY‐VCH Verlag
Témata:
Anti-Infective Agents - administration & dosage
Anti-Infective Agents - pharmacokinetics
confocal Raman microspectroscopy
depth correction
Dermatologic Agents - pharmacokinetics
Diffusion
drug tracking
human skin
Humans
In Vitro Techniques
mathematical modeling
Metronidazole - administration & dosage
Metronidazole - pharmacokinetics
Models, Biological
Permeability
Skin - metabolism
Skin Absorption
Spectrum Analysis, Raman - methods
ISSN:1864-063X, 1864-0648, 1864-0648
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Shrnutí:Confocal Raman microspectroscopy represents the advantage of giving structural and conformational information on samples without any destructive treatment. Recently, several studies were achieved to study the skin hydration, endogenous and exogenous molecules repartition in the skin using the confocal feature of this technique. Meanwhile, when working through a material boundary with a different refractive index, the main limitation remains the spatial precision, especially the distortion in the depth and the depth resolution. Recently, several authors described mathematical models to correct the depth and the resolution values. In this study, we combined theoretical approaches, proposed by different authors with experimental measurements to try to find out the most appropriate approach for correction. We then applied the corrections on in‐depth profiles tracking the penetration of Metronidazole, a drug produced by Galderma for rosacea treatment, through excised human skin. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Bibliografie:istex:9A94ABF9BF1C6688CDCDE4D3BD40E0CB0C3E0381
ArticleID:JBIO200710004
ark:/67375/WNG-717Z21D1-0
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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ISSN:1864-063X
1864-0648
1864-0648
DOI:10.1002/jbio.200710004