Assessing the prevalence of mycoplasma contamination in cell culture via a survey of NCBI's RNA-seq archive

Mycoplasmas are notorious contaminants of cell culture and can have profound effects on host cell biology by depriving cells of nutrients and inducing global changes in gene expression. Over the last two decades, sentinel testing has revealed wide-ranging contamination rates in mammalian culture. To...

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Vydáno v:	Nucleic acids research Ročník 43; číslo 5; s. 2535 - 2542
Hlavní autoři:	Olarerin-George, Anthony O., Hogenesch, John B.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	England Oxford University Press 11.03.2015
Témata:	Animals Cell Line Cell Line, Tumor Cells, Cultured - microbiology Computational Biology - methods Data Resources and Analyses Databases, Nucleic Acid Host-Pathogen Interactions - genetics Humans Mycoplasma Mycoplasma - genetics Mycoplasma - physiology Oligonucleotide Array Sequence Analysis - methods Oligonucleotide Array Sequence Analysis - statistics & numerical data Risk Assessment - methods Risk Assessment - statistics & numerical data RNA - genetics RNA, Bacterial - genetics Sequence Alignment - methods Transcriptome - genetics
ISSN:	0305-1048, 1362-4962, 1362-4962
On-line přístup:	Získat plný text
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