Flow Cytometric Analysis of the Oxidative Status in Human Peripheral Blood Mononuclear Cells of Workers Exposed to Welding Fumes

Flow cytometry is a simple analytical technique that identifies, counts, and characterizes cells. The oxidative status of cells is influenced by many exogenous sources, such as occupational exposure to welding fumes. This study evaluated flow cytometry as a method to determine the oxidative status o...

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Bibliographic Details
Published in:Journal of occupational and environmental hygiene Vol. 7; no. 6; pp. 367 - 374
Main Authors: du Plessis, Lissinda, Laubscher, Petrus, Jooste, Jacques, du Plessis, Johan, Franken, Anja, van Aarde, Nico, Eloff, Fritz
Format: Journal Article
Language:English
Published: England Taylor & Francis Group 01.06.2010
Taylor & Francis LLC
Subjects:
Adult
Air Pollutants, Occupational - adverse effects
Antioxidants
Cells
Cellular
Exposure
Flow Cytometry
Fumes
glutathione
Glutathione - blood
Human exposure
Humans
Leukocytes, Mononuclear - metabolism
Lipid Peroxidation
Lipids
Male
Mathematical analysis
Measurement
Microscopy, Confocal
Middle Aged
Occupational
Occupational exposure
Occupational safety
Oxidative stress
Oxidative Stress - physiology
Pilot Projects
reactive oxygen species
Reactive Oxygen Species - blood
Stress ratio
Studies
Welding
welding fume
Welding fumes
Young Adult
ISSN:1545-9624, 1545-9632, 1545-9632
Online Access:Get full text
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Summary:Flow cytometry is a simple analytical technique that identifies, counts, and characterizes cells. The oxidative status of cells is influenced by many exogenous sources, such as occupational exposure to welding fumes. This study evaluated flow cytometry as a method to determine the oxidative status of male welders (n = 15) occupationally exposed to welding fumes. Flow cytometric analysis of reactive oxygen species (ROS) was carried out in peripheral blood mononuclear cells (PBMC) by using the probe 2, 7-dichlorodihydrofluorescein diacetate (DCFH-DA). Lipid peroxidation was measured by the decrease of fluor-DHPE fluorescence and intracellular glutathione (GSH) levels by using mercury orange. All of the parameters were also observed under a confocal microscope. The oxidative stress ratio was calculated from the oxidative damage and the antioxidant capacity to give an accurate account of the cellular oxidative status. ROS and lipid peroxidation levels were elevated by ∼ 87% and ∼ 96%, respectively, and GSH levels lowered ∼ 96% in PBMC of workers exposed to welding fumes compared with non-exposed controls. The oxidative stress ratio was significantly higher (p < 0.001) in the exposed group. Flow cytometry can be useful for the measurement of cellular oxidative stress in somatic cells of workers exposed to welding fumes and other occupational settings. Calculating an oxidative stress index may be useful in predicting disease outcomes and whether preventative control measures are efficient.
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ISSN:1545-9624
1545-9632
1545-9632
DOI:10.1080/15459621003724108