Involvement of Wnt, Eda and Shh at defined stages of sweat gland development
To maintain body temperature, sweat glands develop from embryonic ectoderm by a poorly defined mechanism. We demonstrate a temporal cascade of regulation during mouse sweat gland formation. Sweat gland induction failed completely when canonical Wnt signaling was blocked in skin epithelium, and was a...
Uloženo v:
| Vydáno v: | Development (Cambridge) Ročník 141; číslo 19; s. 3752 |
|---|---|
| Hlavní autoři: | , , , , , , |
| Médium: | Journal Article |
| Jazyk: | angličtina |
| Vydáno: |
England
01.10.2014
|
| Témata: | |
| ISSN: | 1477-9129, 1477-9129 |
| On-line přístup: | Zjistit podrobnosti o přístupu |
| Tagy: |
Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
|
| Abstract | To maintain body temperature, sweat glands develop from embryonic ectoderm by a poorly defined mechanism. We demonstrate a temporal cascade of regulation during mouse sweat gland formation. Sweat gland induction failed completely when canonical Wnt signaling was blocked in skin epithelium, and was accompanied by sharp downregulation of downstream Wnt, Eda and Shh pathway genes. The Wnt antagonist Dkk4 appeared to inhibit this induction: Dkk4 was sharply downregulated in β-catenin-ablated mice, indicating that it is induced by Wnt/β-catenin; however, its overexpression repressed Wnt target genes and significantly reduced gland numbers. Eda signaling succeeded Wnt. Wnt signaling was still active and nascent sweat gland pre-germs were still seen in Eda-null mice, but the pre-germs failed to develop further and the downstream Shh pathway was not activated. When Wnt and Eda were intact but Shh was ablated, germ induction and subsequent duct formation occurred normally, but the final stage of secretory coil formation failed. Thus, sweat gland development shows a relay of regulatory steps initiated by Wnt/β-catenin - itself modulated by Dkk4 - with subsequent participation of Eda and Shh pathways. |
|---|---|
| AbstractList | To maintain body temperature, sweat glands develop from embryonic ectoderm by a poorly defined mechanism. We demonstrate a temporal cascade of regulation during mouse sweat gland formation. Sweat gland induction failed completely when canonical Wnt signaling was blocked in skin epithelium, and was accompanied by sharp downregulation of downstream Wnt, Eda and Shh pathway genes. The Wnt antagonist Dkk4 appeared to inhibit this induction: Dkk4 was sharply downregulated in β-catenin-ablated mice, indicating that it is induced by Wnt/β-catenin; however, its overexpression repressed Wnt target genes and significantly reduced gland numbers. Eda signaling succeeded Wnt. Wnt signaling was still active and nascent sweat gland pre-germs were still seen in Eda-null mice, but the pre-germs failed to develop further and the downstream Shh pathway was not activated. When Wnt and Eda were intact but Shh was ablated, germ induction and subsequent duct formation occurred normally, but the final stage of secretory coil formation failed. Thus, sweat gland development shows a relay of regulatory steps initiated by Wnt/β-catenin - itself modulated by Dkk4 - with subsequent participation of Eda and Shh pathways.To maintain body temperature, sweat glands develop from embryonic ectoderm by a poorly defined mechanism. We demonstrate a temporal cascade of regulation during mouse sweat gland formation. Sweat gland induction failed completely when canonical Wnt signaling was blocked in skin epithelium, and was accompanied by sharp downregulation of downstream Wnt, Eda and Shh pathway genes. The Wnt antagonist Dkk4 appeared to inhibit this induction: Dkk4 was sharply downregulated in β-catenin-ablated mice, indicating that it is induced by Wnt/β-catenin; however, its overexpression repressed Wnt target genes and significantly reduced gland numbers. Eda signaling succeeded Wnt. Wnt signaling was still active and nascent sweat gland pre-germs were still seen in Eda-null mice, but the pre-germs failed to develop further and the downstream Shh pathway was not activated. When Wnt and Eda were intact but Shh was ablated, germ induction and subsequent duct formation occurred normally, but the final stage of secretory coil formation failed. Thus, sweat gland development shows a relay of regulatory steps initiated by Wnt/β-catenin - itself modulated by Dkk4 - with subsequent participation of Eda and Shh pathways. To maintain body temperature, sweat glands develop from embryonic ectoderm by a poorly defined mechanism. We demonstrate a temporal cascade of regulation during mouse sweat gland formation. Sweat gland induction failed completely when canonical Wnt signaling was blocked in skin epithelium, and was accompanied by sharp downregulation of downstream Wnt, Eda and Shh pathway genes. The Wnt antagonist Dkk4 appeared to inhibit this induction: Dkk4 was sharply downregulated in β-catenin-ablated mice, indicating that it is induced by Wnt/β-catenin; however, its overexpression repressed Wnt target genes and significantly reduced gland numbers. Eda signaling succeeded Wnt. Wnt signaling was still active and nascent sweat gland pre-germs were still seen in Eda-null mice, but the pre-germs failed to develop further and the downstream Shh pathway was not activated. When Wnt and Eda were intact but Shh was ablated, germ induction and subsequent duct formation occurred normally, but the final stage of secretory coil formation failed. Thus, sweat gland development shows a relay of regulatory steps initiated by Wnt/β-catenin - itself modulated by Dkk4 - with subsequent participation of Eda and Shh pathways. |
| Author | Piao, Yulan Yin, Mingzhu Cui, Chang-Yi Sima, Jian Michel, Marc Schlessinger, David Childress, Victoria |
| Author_xml | – sequence: 1 givenname: Chang-Yi surname: Cui fullname: Cui, Chang-Yi email: cuic@mail.nih.gov organization: Laboratory of Genetics, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA cuic@mail.nih.gov – sequence: 2 givenname: Mingzhu surname: Yin fullname: Yin, Mingzhu organization: Laboratory of Genetics, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA – sequence: 3 givenname: Jian surname: Sima fullname: Sima, Jian organization: Laboratory of Genetics, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA – sequence: 4 givenname: Victoria surname: Childress fullname: Childress, Victoria organization: Laboratory of Genetics, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA – sequence: 5 givenname: Marc surname: Michel fullname: Michel, Marc organization: Laboratory of Genetics, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA – sequence: 6 givenname: Yulan surname: Piao fullname: Piao, Yulan organization: Laboratory of Genetics, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA – sequence: 7 givenname: David surname: Schlessinger fullname: Schlessinger, David organization: Laboratory of Genetics, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/25249463$$D View this record in MEDLINE/PubMed |
| BookMark | eNpNkF1LwzAYhYNM3Ife-AMkl17YmY8maS5lTB0MvHDgZUmbN1ulTWrTTfbv7XCCV-fl8HDew5mikQ8eELqlZE5Zyh4tHOaUaMbpBZrQVKlEU6ZH_-4xmsb4SQjhUqkrNGaCpTqVfILWK38I9QEa8D0ODn_4_gEvrcHGW_y-22HTYwuu8mBx7M0W4omK3zD42_oEDd-hDu0p4BpdOlNHuDnrDG2el5vFa7J-e1ktntZJORTqk0yWsnCSqKKgqTSCGqYtV0JxxzLHC8tKozLQttTSOJ6KgdNOUVtoTaxkM3T_G9t24WsPsc-bKpZQD3Ug7GNOhRSCUKnZgN6d0X3RgM3brmpMd8z_BmA_cJRdVQ |
| CitedBy_id | crossref_primary_10_3389_fgene_2022_1000744 crossref_primary_10_1016_j_scib_2024_11_003 crossref_primary_10_1186_s40779_022_00372_5 crossref_primary_10_1016_j_bbrc_2017_06_138 crossref_primary_10_1016_j_gene_2020_144338 crossref_primary_10_1073_pnas_1511680112 crossref_primary_10_1111_age_12896 crossref_primary_10_3390_biom13121702 crossref_primary_10_1080_23311932_2018_1552552 crossref_primary_10_1111_ahg_70014 crossref_primary_10_1111_exd_13062 crossref_primary_10_1007_s10735_021_10023_z crossref_primary_10_3390_ijms21249736 crossref_primary_10_1111_exd_12773 crossref_primary_10_1038_s41419_019_1485_5 crossref_primary_10_1016_j_gene_2020_144968 crossref_primary_10_3389_fgene_2018_00739 crossref_primary_10_1139_gen_2021_0030 crossref_primary_10_5713_ab_23_0525 crossref_primary_10_1371_journal_pone_0284047 crossref_primary_10_1016_j_jid_2017_03_003 crossref_primary_10_3390_ijms23168911 crossref_primary_10_1186_s12862_022_01984_0 crossref_primary_10_1038_s41419_019_1503_7 crossref_primary_10_1242_dev_143909 crossref_primary_10_1002_advs_202103079 crossref_primary_10_3389_fcell_2021_667765 crossref_primary_10_1111_brv_70067 crossref_primary_10_1126_science_aah6102 crossref_primary_10_1007_s10561_016_9548_7 crossref_primary_10_1111_jcmm_13311 crossref_primary_10_1242_dev_197657 crossref_primary_10_1097_CM9_0000000000002076 crossref_primary_10_1371_journal_pgen_1005676 crossref_primary_10_1016_j_biomaterials_2020_120201 crossref_primary_10_1111_asj_12851 crossref_primary_10_1038_ncomms15397 crossref_primary_10_1016_j_joca_2025_02_789 crossref_primary_10_1111_exd_14096 crossref_primary_10_1016_j_devcel_2023_11_015 crossref_primary_10_1093_burnst_tkaf016 crossref_primary_10_1371_journal_pgen_1010614 crossref_primary_10_1016_j_diff_2025_100838 crossref_primary_10_1111_exd_14415 crossref_primary_10_3389_fphys_2022_1033130 crossref_primary_10_1371_journal_pone_0216249 crossref_primary_10_3389_fnana_2024_1454095 crossref_primary_10_1016_j_livsci_2022_105028 crossref_primary_10_1002_dvg_23532 crossref_primary_10_3390_cimb46080487 crossref_primary_10_1016_j_jid_2023_02_007 |
| ContentType | Journal Article |
| Copyright | 2014. Published by The Company of Biologists Ltd. |
| Copyright_xml | – notice: 2014. Published by The Company of Biologists Ltd. |
| DBID | CGR CUY CVF ECM EIF NPM 7X8 |
| DOI | 10.1242/dev.109231 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | no_fulltext_linktorsrc |
| Discipline | Medicine Zoology Biology |
| EISSN | 1477-9129 |
| ExternalDocumentID | 25249463 |
| Genre | Journal Article Research Support, N.I.H., Intramural |
| GrantInformation_xml | – fundername: Intramural NIH HHS |
| GroupedDBID | --- -DZ -ET -~X .55 .GJ 0R~ 186 18M 2WC 34G 39C 3O- 4.4 53G 5GY 5RE 5VS 85S 9M8 AAFWJ ABJNI ABTAH ABZEH ACGFS ACPRK ACREN ADBBV ADFRT ADVGF AENEX AFFNX AGCDD AGGIJ AI. ALMA_UNASSIGNED_HOLDINGS AMTXH BAWUL BTFSW C1A CGR CS3 CUY CVF DIK DU5 E3Z EBS ECM EIF EJD F5P F9R GX1 H13 HZ~ H~9 INIJC KQ8 MVM NPM O9- OHT OK1 P2P R.V RCB RHF RHI SJN TR2 TWZ UPT UQL VH1 VXZ W8F WH7 WOQ X7M XJT XOL XSW ZCG ZGI ZXP ZY4 7X8 ADXHL AETEA |
| ID | FETCH-LOGICAL-c477t-86c6bf607bb146a51a29d37573f28f3bd2ca78e9dc96af345bb19f71db990d62 |
| IEDL.DBID | 7X8 |
| ISICitedReferencesCount | 60 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000343418800015&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 1477-9129 |
| IngestDate | Thu Sep 04 18:18:19 EDT 2025 Wed Feb 19 02:33:28 EST 2025 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 19 |
| Keywords | Hyperhidrosis Mouse Exocrine gland Hair follicle Heatstroke Ectodermal dysplasia Skin appendage |
| Language | English |
| License | 2014. Published by The Company of Biologists Ltd. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c477t-86c6bf607bb146a51a29d37573f28f3bd2ca78e9dc96af345bb19f71db990d62 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| OpenAccessLink | https://www.ncbi.nlm.nih.gov/pmc/articles/4197578 |
| PMID | 25249463 |
| PQID | 1565501692 |
| PQPubID | 23479 |
| ParticipantIDs | proquest_miscellaneous_1565501692 pubmed_primary_25249463 |
| PublicationCentury | 2000 |
| PublicationDate | 2014-Oct 20141001 |
| PublicationDateYYYYMMDD | 2014-10-01 |
| PublicationDate_xml | – month: 10 year: 2014 text: 2014-Oct |
| PublicationDecade | 2010 |
| PublicationPlace | England |
| PublicationPlace_xml | – name: England |
| PublicationTitle | Development (Cambridge) |
| PublicationTitleAlternate | Development |
| PublicationYear | 2014 |
| SSID | ssj0003677 |
| Score | 2.401161 |
| Snippet | To maintain body temperature, sweat glands develop from embryonic ectoderm by a poorly defined mechanism. We demonstrate a temporal cascade of regulation... |
| SourceID | proquest pubmed |
| SourceType | Aggregation Database Index Database |
| StartPage | 3752 |
| SubjectTerms | Animals beta Catenin - deficiency DNA Primers - genetics Ectodysplasins - metabolism Fluorescent Antibody Technique Galactosides Gene Expression Profiling Gene Expression Regulation, Developmental - genetics Gene Expression Regulation, Developmental - physiology Hedgehog Proteins - metabolism In Situ Hybridization Indoles Intercellular Signaling Peptides and Proteins - genetics Intercellular Signaling Peptides and Proteins - metabolism Mice Mice, Transgenic Real-Time Polymerase Chain Reaction Sweat Glands - embryology Sweat Glands - metabolism Wnt Proteins - metabolism Wnt Signaling Pathway - physiology |
| Title | Involvement of Wnt, Eda and Shh at defined stages of sweat gland development |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/25249463 https://www.proquest.com/docview/1565501692 |
| Volume | 141 |
| WOSCitedRecordID | wos000343418800015&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1LS8NAEF7UqnjxUV_1xQoeDW2SzW5yEpEWBVsKFixeymZ3x3pJqokV_72zm5RePAheclh2QxgmM9_sDN9HyFXMRYelKvIET7BA4Ux6lgLF8wEMB-gwCeDEJsRgEI_HybC-cCvqscpFTHSBWufK3pG3sc5AMO3zJLiZvXtWNcp2V2sJjVXSCBHKWK8W4yVbeMid8qLPbJsSE1tNT4pZqa3N3DIpBbW43K_Q0qWY3s5_P26XbNfgkt5W3rBHVkzWJBuV3OR3k2z260Y6Lr7kbnGfPD5kGKEca3hJc6DPWXlNu1pSmWn6NJ1SWVJtAE9pikjy1RR2V_GFMZw6_Q-ql3NHB2TU647u7r1aYsFTaJTSi7niKfCOSFMMmTLyZZDoUEQihCCGMNWBkiI2iVYJlxCyCPclIHydYhbTPDgka1memWNCOVjkhFtixfFNOhUasLRRKlGKMdAtcrkw3QQ92LYlZGbyz2KyNF6LHFX2n8wqqo1JEGF5yHh48ofTp2QL0QyrJu3OSAPw_zXnZF3Ny7fi48K5Bj4Hw_4PxFTCpQ |
| linkProvider | ProQuest |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Involvement+of+Wnt%2C+Eda+and+Shh+at+defined+stages+of+sweat+gland+development&rft.jtitle=Development+%28Cambridge%29&rft.au=Cui%2C+Chang-Yi&rft.au=Yin%2C+Mingzhu&rft.au=Sima%2C+Jian&rft.au=Childress%2C+Victoria&rft.date=2014-10-01&rft.eissn=1477-9129&rft.volume=141&rft.issue=19&rft.spage=3752&rft_id=info:doi/10.1242%2Fdev.109231&rft_id=info%3Apmid%2F25249463&rft_id=info%3Apmid%2F25249463&rft.externalDocID=25249463 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1477-9129&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1477-9129&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1477-9129&client=summon |