TRIM8-associated non-coding RNA panel as a biomarker for Lupus nephritis activity

Background Lupus nephritis (LN) represents a major complication in systemic lupus erythematosus (SLE). The objective of this study was to evaluate the TRIM8 gene and its associated non-coding RNAs ( lnc-SSBP2-1:1 and hsa-miR-126-5p ) as potential non-invasive biomarkers for LN activity. Methods Bioi...

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Vydané v:Journal of translational medicine Ročník 23; číslo 1; s. 1229 - 10
Hlavní autori: Elgawad, Mostafa Abdelnasier Abd, Shinnawy, Howayda Abdelhamid El, Eissa, Sanaa, Ali, Nouran Abdelfattah Sayed, Behairy, Maha Abdelmoneim, Kamel, Cherry Reda, Kamel, Marwa Mostafa
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: London BioMed Central 05.11.2025
BioMed Central Ltd
BMC
Predmet:
Adult
Analysis
B cells
Biological markers
Biomarkers
Biomarkers - blood
Biomarkers - metabolism
Biomedical and Life Sciences
Biomedicine
Care and treatment
Carrier Proteins - genetics
Carrier Proteins - metabolism
Case-Control Studies
Complications and side effects
Development and progression
Diagnosis
Ethylenediaminetetraacetic acid
Female
hsa-miR-126-5p
Humans
Immune response
Immunobiology and immunotherapy
lnc-SSBP2-1:1
Lupus
Lupus nephritis
Lupus Nephritis - blood
Lupus Nephritis - genetics
Male
Medicine/Public Health
MicroRNAs - genetics
MicroRNAs - metabolism
Middle Aged
Nephritis
Nerve Tissue Proteins
non-coding RNA network
Polymerase chain reaction
Risk factors
RNA
RNA, Long Noncoding - genetics
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA, Untranslated - genetics
RNA, Untranslated - metabolism
Systemic lupus erythematosus
Testing
TRIM8
Tripartite Motif Proteins
Egypt
Biomarkers
Disease activity
Lupus nephritis
non-coding RNA network
TRIM8
hsa-miR-126-5p
lnc-SSBP2-1:1
ISSN:1479-5876, 1479-5876
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Shrnutí:Background Lupus nephritis (LN) represents a major complication in systemic lupus erythematosus (SLE). The objective of this study was to evaluate the TRIM8 gene and its associated non-coding RNAs ( lnc-SSBP2-1:1 and hsa-miR-126-5p ) as potential non-invasive biomarkers for LN activity. Methods Bioinformatics analyses were initially employed to identify candidate mRNA and associated non-coding RNAs (ncRNAs) implicated in LN. Expression profiles of TRIM8lnc-SSBP2-1:1 and hsa-miR-126-5p were validated in blood samples from 40 active LN, 30 inactive LN patients, and 20 healthy individuals via real-time PCR. Results TRIM8 mRNA and lnc-SSBP2-1:1 lncRNA levels were notably upregulated in active LN ( p  < 0.001), while hsa-miR-126-5p was reduced ( p  < 0.001). SLEDAI-2K scores correlated positively with TRIM8 mRNA and lnc-SSBP2-1:1 , and negatively with hsa-miR-126-5p . Conclusions This study highlights TRIM8 -associated ncRNA regulatory network as promising biomarkers in LN activωity, with potential clinical impact. Graphical abstract Highlights TRIM8 and lnc-SSBP2-1:1 are upregulated, hsa-miR-126-5p is downregulated in active LN Biomarkers strongly correlated with SLEDAI-2K disease activity scores High diagnostic accuracy (AUCs > 0.93) in distinguishing active vs inactive LN Potential non-invasive tool to complement current LN monitoring
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1479-5876
1479-5876
DOI:10.1186/s12967-025-07137-3