TRIM8-associated non-coding RNA panel as a biomarker for Lupus nephritis activity

Background Lupus nephritis (LN) represents a major complication in systemic lupus erythematosus (SLE). The objective of this study was to evaluate the TRIM8 gene and its associated non-coding RNAs ( lnc-SSBP2-1:1 and hsa-miR-126-5p ) as potential non-invasive biomarkers for LN activity. Methods Bioi...

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Vydané v:	Journal of translational medicine Ročník 23; číslo 1; s. 1229 - 10
Hlavní autori:	Elgawad, Mostafa Abdelnasier Abd, Shinnawy, Howayda Abdelhamid El, Eissa, Sanaa, Ali, Nouran Abdelfattah Sayed, Behairy, Maha Abdelmoneim, Kamel, Cherry Reda, Kamel, Marwa Mostafa
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	London BioMed Central 05.11.2025 BioMed Central Ltd BMC
Predmet:	Adult Analysis B cells Biological markers Biomarkers Biomarkers - blood Biomarkers - metabolism Biomedical and Life Sciences Biomedicine Care and treatment Carrier Proteins - genetics Carrier Proteins - metabolism Case-Control Studies Complications and side effects Development and progression Diagnosis Ethylenediaminetetraacetic acid Female hsa-miR-126-5p Humans Immune response Immunobiology and immunotherapy lnc-SSBP2-1:1 Lupus Lupus nephritis Lupus Nephritis - blood Lupus Nephritis - genetics Male Medicine/Public Health MicroRNAs - genetics MicroRNAs - metabolism Middle Aged Nephritis Nerve Tissue Proteins non-coding RNA network Polymerase chain reaction Risk factors RNA RNA, Long Noncoding - genetics RNA, Messenger - genetics RNA, Messenger - metabolism RNA, Untranslated - genetics RNA, Untranslated - metabolism Systemic lupus erythematosus Testing TRIM8 Tripartite Motif Proteins Egypt Biomarkers Disease activity Lupus nephritis non-coding RNA network TRIM8 hsa-miR-126-5p lnc-SSBP2-1:1
ISSN:	1479-5876, 1479-5876
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Popis
Shrnutí:	Background Lupus nephritis (LN) represents a major complication in systemic lupus erythematosus (SLE). The objective of this study was to evaluate the TRIM8 gene and its associated non-coding RNAs ( lnc-SSBP2-1:1 and hsa-miR-126-5p ) as potential non-invasive biomarkers for LN activity. Methods Bioinformatics analyses were initially employed to identify candidate mRNA and associated non-coding RNAs (ncRNAs) implicated in LN. Expression profiles of TRIM8lnc-SSBP2-1:1 and hsa-miR-126-5p were validated in blood samples from 40 active LN, 30 inactive LN patients, and 20 healthy individuals via real-time PCR. Results TRIM8 mRNA and lnc-SSBP2-1:1 lncRNA levels were notably upregulated in active LN ( p < 0.001), while hsa-miR-126-5p was reduced ( p < 0.001). SLEDAI-2K scores correlated positively with TRIM8 mRNA and lnc-SSBP2-1:1 , and negatively with hsa-miR-126-5p . Conclusions This study highlights TRIM8 -associated ncRNA regulatory network as promising biomarkers in LN activωity, with potential clinical impact. Graphical abstract Highlights TRIM8 and lnc-SSBP2-1:1 are upregulated, hsa-miR-126-5p is downregulated in active LN Biomarkers strongly correlated with SLEDAI-2K disease activity scores High diagnostic accuracy (AUCs > 0.93) in distinguishing active vs inactive LN Potential non-invasive tool to complement current LN monitoring
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1479-5876 1479-5876
DOI:	10.1186/s12967-025-07137-3