Targeted Brain Tumor Radiotherapy Using an Auger Emitter

Glioblastoma multiforme is a highly aggressive form of brain cancer whose location, tendency to infiltrate healthy surrounding tissue, and heterogeneity significantly limit survival, with scant progress having been made in recent decades. I-MAPi (Iodine-123 Meitner-Auger PARP1 inhibitor) is a precis...

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Veröffentlicht in:Clinical cancer research Jg. 26; H. 12; S. 2871
Hauptverfasser: Pirovano, Giacomo, Jannetti, Stephen A, Carter, Lukas M, Sadique, Ahmad, Kossatz, Susanne, Guru, Navjot, Demétrio De Souza França, Paula, Maeda, Masatomo, Zeglis, Brian M, Lewis, Jason S, Humm, John L, Reiner, Thomas
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Sprache:Englisch
Veröffentlicht: United States 15.06.2020
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ISSN:1557-3265, 1557-3265
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Abstract Glioblastoma multiforme is a highly aggressive form of brain cancer whose location, tendency to infiltrate healthy surrounding tissue, and heterogeneity significantly limit survival, with scant progress having been made in recent decades. I-MAPi (Iodine-123 Meitner-Auger PARP1 inhibitor) is a precise therapeutic tool composed of a PARP1 inhibitor radiolabeled with an Auger- and gamma-emitting iodine isotope. Here, the PARP inhibitor, which binds to the DNA repair enzyme PARP1, specifically targets cancer cells, sparing healthy tissue, and carries a radioactive payload within reach of the cancer cells' DNA. The high relative biological efficacy of Auger electrons within their short range of action is leveraged to inflict DNA damage and cell death with high precision. The gamma ray emission of I-MAPi allows for the imaging of tumor progression and therapy response, and for patient dosimetry calculation. Here we demonstrated the efficacy and specificity of this small-molecule radiotheranostic in a complex preclinical model. and studies demonstrate high tumor uptake and a prolonged survival in mice treated with I-MAPi when compared with vehicle controls. Different methods of drug delivery were investigated to develop this technology for clinical applications, including convection enhanced delivery and intrathecal injection. Taken together, these results represent the first full characterization of an Auger-emitting PARP inhibitor which demonstrate a survival benefit in mouse models of GBM and confirm the high potential of I-MAPi for clinical translation.
AbstractList Glioblastoma multiforme is a highly aggressive form of brain cancer whose location, tendency to infiltrate healthy surrounding tissue, and heterogeneity significantly limit survival, with scant progress having been made in recent decades. I-MAPi (Iodine-123 Meitner-Auger PARP1 inhibitor) is a precise therapeutic tool composed of a PARP1 inhibitor radiolabeled with an Auger- and gamma-emitting iodine isotope. Here, the PARP inhibitor, which binds to the DNA repair enzyme PARP1, specifically targets cancer cells, sparing healthy tissue, and carries a radioactive payload within reach of the cancer cells' DNA. The high relative biological efficacy of Auger electrons within their short range of action is leveraged to inflict DNA damage and cell death with high precision. The gamma ray emission of I-MAPi allows for the imaging of tumor progression and therapy response, and for patient dosimetry calculation. Here we demonstrated the efficacy and specificity of this small-molecule radiotheranostic in a complex preclinical model. and studies demonstrate high tumor uptake and a prolonged survival in mice treated with I-MAPi when compared with vehicle controls. Different methods of drug delivery were investigated to develop this technology for clinical applications, including convection enhanced delivery and intrathecal injection. Taken together, these results represent the first full characterization of an Auger-emitting PARP inhibitor which demonstrate a survival benefit in mouse models of GBM and confirm the high potential of I-MAPi for clinical translation.
Glioblastoma multiforme is a highly aggressive form of brain cancer whose location, tendency to infiltrate healthy surrounding tissue, and heterogeneity significantly limit survival, with scant progress having been made in recent decades.PURPOSEGlioblastoma multiforme is a highly aggressive form of brain cancer whose location, tendency to infiltrate healthy surrounding tissue, and heterogeneity significantly limit survival, with scant progress having been made in recent decades.123I-MAPi (Iodine-123 Meitner-Auger PARP1 inhibitor) is a precise therapeutic tool composed of a PARP1 inhibitor radiolabeled with an Auger- and gamma-emitting iodine isotope. Here, the PARP inhibitor, which binds to the DNA repair enzyme PARP1, specifically targets cancer cells, sparing healthy tissue, and carries a radioactive payload within reach of the cancer cells' DNA.EXPERIMENTAL DESIGN123I-MAPi (Iodine-123 Meitner-Auger PARP1 inhibitor) is a precise therapeutic tool composed of a PARP1 inhibitor radiolabeled with an Auger- and gamma-emitting iodine isotope. Here, the PARP inhibitor, which binds to the DNA repair enzyme PARP1, specifically targets cancer cells, sparing healthy tissue, and carries a radioactive payload within reach of the cancer cells' DNA.The high relative biological efficacy of Auger electrons within their short range of action is leveraged to inflict DNA damage and cell death with high precision. The gamma ray emission of 123I-MAPi allows for the imaging of tumor progression and therapy response, and for patient dosimetry calculation. Here we demonstrated the efficacy and specificity of this small-molecule radiotheranostic in a complex preclinical model. In vitro and in vivo studies demonstrate high tumor uptake and a prolonged survival in mice treated with 123I-MAPi when compared with vehicle controls. Different methods of drug delivery were investigated to develop this technology for clinical applications, including convection enhanced delivery and intrathecal injection.RESULTSThe high relative biological efficacy of Auger electrons within their short range of action is leveraged to inflict DNA damage and cell death with high precision. The gamma ray emission of 123I-MAPi allows for the imaging of tumor progression and therapy response, and for patient dosimetry calculation. Here we demonstrated the efficacy and specificity of this small-molecule radiotheranostic in a complex preclinical model. In vitro and in vivo studies demonstrate high tumor uptake and a prolonged survival in mice treated with 123I-MAPi when compared with vehicle controls. Different methods of drug delivery were investigated to develop this technology for clinical applications, including convection enhanced delivery and intrathecal injection.Taken together, these results represent the first full characterization of an Auger-emitting PARP inhibitor which demonstrate a survival benefit in mouse models of GBM and confirm the high potential of 123I-MAPi for clinical translation.CONCLUSIONSTaken together, these results represent the first full characterization of an Auger-emitting PARP inhibitor which demonstrate a survival benefit in mouse models of GBM and confirm the high potential of 123I-MAPi for clinical translation.
Author Demétrio De Souza França, Paula
Maeda, Masatomo
Pirovano, Giacomo
Jannetti, Stephen A
Carter, Lukas M
Guru, Navjot
Kossatz, Susanne
Humm, John L
Sadique, Ahmad
Reiner, Thomas
Lewis, Jason S
Zeglis, Brian M
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  orcidid: 0000-0001-8862-3613
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  fullname: Carter, Lukas M
  organization: Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York
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  surname: Sadique
  fullname: Sadique, Ahmad
  organization: Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York
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  organization: Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York
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  organization: Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York
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  orcidid: 0000-0003-4316-8834
  surname: Demétrio De Souza França
  fullname: Demétrio De Souza França, Paula
  organization: Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York
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  surname: Maeda
  fullname: Maeda, Masatomo
  organization: Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York
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  givenname: Brian M
  surname: Zeglis
  fullname: Zeglis, Brian M
  organization: PhD Program in Chemistry, The Graduate Center, The City University of New York (CUNY), New York, New York
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  givenname: Jason S
  orcidid: 0000-0001-7065-4534
  surname: Lewis
  fullname: Lewis, Jason S
  organization: Department of Pharmacology, Weill Cornell Medical College, New York, New York
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  givenname: John L
  surname: Humm
  fullname: Humm, John L
  organization: Department of Medical Physics, Memorial Sloan Kettering Cancer Center, New York, New York
– sequence: 12
  givenname: Thomas
  surname: Reiner
  fullname: Reiner, Thomas
  email: reinert@mskcc.org
  organization: Chemical Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York
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Snippet Glioblastoma multiforme is a highly aggressive form of brain cancer whose location, tendency to infiltrate healthy surrounding tissue, and heterogeneity...
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SubjectTerms Animals
Apoptosis
Brain Neoplasms - drug therapy
Brain Neoplasms - pathology
Brain Neoplasms - radiotherapy
Cell Proliferation
Female
Glioblastoma - drug therapy
Glioblastoma - pathology
Glioblastoma - radiotherapy
Humans
Iodine Radioisotopes - therapeutic use
Mice
Mice, Nude
Poly (ADP-Ribose) Polymerase-1 - antagonists & inhibitors
Poly(ADP-ribose) Polymerase Inhibitors - pharmacology
Radiotherapy - methods
Tumor Cells, Cultured
Xenograft Model Antitumor Assays
Title Targeted Brain Tumor Radiotherapy Using an Auger Emitter
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