Mobile Laboratory Reveals the Circulation of Dengue Virus Serotype I of Asian Origin in Medina Gounass (Guediawaye), Senegal

With the growing success of controlling malaria in Sub-Saharan Africa, the incidence of fever due to malaria is in decline, whereas the proportion of patients with non-malaria febrile illness (NMFI) is increasing. Clinical diagnosis of NMFI is hampered by unspecific symptoms, but early diagnosis is...

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Vydáno v:Diagnostics (Basel) Ročník 10; číslo 6; s. 408
Hlavní autoři: Dieng, Idrissa, Hedible, Boris Gildas, Diagne, Moussa Moïse, El Wahed, Ahmed Abd, Diagne, Cheikh Tidiane, Fall, Cheikh, Richard, Vicent, Vray, Muriel, Weidmann, Manfred, Faye, Ousmane, Sall, Amadou Alpha, Faye, Oumar
Médium: Journal Article
Jazyk:angličtina
Vydáno: Basel MDPI AG 16.06.2020
MDPI
Témata:
Antibodies
Antigens
Brief Report
Dengue fever
DENV
Diagnostic tests
fever
Laboratories
Malaria
mobile laboratory
Mortality
NMFI
RPA
Tropical diseases
Viruses
Senegal
Germany
Africa
ISSN:2075-4418, 2075-4418
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Shrnutí:With the growing success of controlling malaria in Sub-Saharan Africa, the incidence of fever due to malaria is in decline, whereas the proportion of patients with non-malaria febrile illness (NMFI) is increasing. Clinical diagnosis of NMFI is hampered by unspecific symptoms, but early diagnosis is a key factor for both better patient care and disease control. The aim of this study was to determine the arboviral aetiologies of NMFI in low resource settings, using a mobile laboratory based on recombinase polymerase amplification (RPA) assays. The panel of tests for this study was expanded to five arboviruses: dengue virus (DENV), zika virus (ZIKV), yellow fever virus (YFV), chikungunya virus (CHIKV), and rift valley fever virus (RVFV). One hundred and four children aged between one month and 115 months were enrolled and screened. Three of the 104 blood samples of children <10 years presented at an outpatient clinic tested positive for DENV. The results were confirmed by RT-PCR, partial sequencing, and non-structural protein 1 (NS1) antigen capture by ELISA (Biorad, France). Phylogenetic analysis of the derived DENV-1 sequences clustered them with sequences of DENV-1 isolated from Guangzhou, China, in 2014. In conclusion, this mobile setup proved reliable for the rapid identification of the causative agent of NMFI, with results consistent with those obtained in the reference laboratory’s settings.
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ISSN:2075-4418
2075-4418
DOI:10.3390/diagnostics10060408