The structural determinants of checkpoint activation

Here, we demonstrate that primed, single-stranded DNA (ssDNA) is sufficient for activation of the ATR-dependent checkpoint pathway in Xenopus egg extracts. Using this structure, we define the contribution of the 5'- and 3'-primer ends to Chk1 activation when replication is blocked and ongo...

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Vydané v:	Genes & development Ročník 21; číslo 8; s. 898
Hlavní autori:	MacDougall, Christina A, Byun, Tony S, Van, Christopher, Yee, Muh-ching, Cimprich, Karlene A
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States 15.04.2007
Predmet:	Animals Ataxia Telangiectasia Mutated Proteins Cell Cycle Cell Cycle Proteins - metabolism Checkpoint Kinase 1 DNA Damage DNA Replication DNA, Single-Stranded - metabolism Ovum - chemistry Phosphorylation Protein Kinases - metabolism Protein-Serine-Threonine Kinases - metabolism S Phase Xenopus laevis Xenopus Proteins - metabolism
ISSN:	0890-9369
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Shrnutí:	Here, we demonstrate that primed, single-stranded DNA (ssDNA) is sufficient for activation of the ATR-dependent checkpoint pathway in Xenopus egg extracts. Using this structure, we define the contribution of the 5'- and 3'-primer ends to Chk1 activation when replication is blocked and ongoing. In addition, we show that although ssDNA is not sufficient for checkpoint activation, the amount of ssDNA adjacent to the primer influences the level of Chk1 phosphorylation. These observations define the minimal DNA requirements for checkpoint activation and suggest that primed ssDNA represents a common checkpoint activating-structure formed following many types of damage.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0890-9369
DOI:	10.1101/gad.1522607