Novel secretory protein Ss-Caf1 of the plant-pathogenic fungus Sclerotinia sclerotiorum is required for host penetration and normal sclerotial development

To decipher the mechanism of pathogenicity in Sclerotinia sclerotiorum, a pathogenicity-defective mutant, Sunf-MT6, was isolated from a T-DNA insertional library. Sunf-MT6 could not form compound appressorium and failed to induce lesions on leaves of rapeseed though it could produce more oxalic acid...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Molecular plant-microbe interactions Jg. 27; H. 1; S. 40 - 55
Hauptverfasser:	Xiao, Xueqiong, Xie, Jiatao, Cheng, Jiasen, Li, Guoqing, Yi, Xianhong, Jiang, Daohong, Fu, Yanping
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States The American Phytopathological Society 01.01.2014
Schlagworte:	Amino Acid Motifs Arabidopsis - microbiology Arabidopsis - physiology Ascomycota - genetics Ascomycota - pathogenicity Ascomycota - physiology Ascomycota - ultrastructure Cell Death Daucus carota - microbiology Daucus carota - physiology Fruiting Bodies, Fungal Fungal Proteins - genetics Fungal Proteins - metabolism Gene Library Gene Silencing Host-Pathogen Interactions Models, Molecular Mutagenesis, Insertional Nicotiana - microbiology Nicotiana - physiology Oxalic Acid - metabolism Phenotype Phylogeny Plant Diseases - microbiology Plant Leaves - microbiology Plant Leaves - physiology Plant Roots - microbiology Plant Roots - physiology Seedlings - microbiology Seedlings - physiology Stress, Physiological
ISSN:	0894-0282, 1943-7706
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Abstract	To decipher the mechanism of pathogenicity in Sclerotinia sclerotiorum, a pathogenicity-defective mutant, Sunf-MT6, was isolated from a T-DNA insertional library. Sunf-MT6 could not form compound appressorium and failed to induce lesions on leaves of rapeseed though it could produce more oxalic acid than the wild-type strain. However, it could enter into host tissues via wounds and cause typical necrotic lesions. Furthermore, Sunf-MT6 produced fewer but larger sclerotia than the wild-type strain Sunf-M. A gene, named Ss-caf1, was disrupted by T-DNA insertion in Sunf-MT6. Gene complementation and knockdown experiments confirmed that the disruption of Ss-caf1 was responsible for the phenotypic changes of Sunf-MT6. Ss-caf1 encodes a secretory protein with a putative Ca(2+)-binding EF-hand motif. High expression levels of Ss-caf1 were observed at an early stage of compound appressorium formation and in immature sclerotia. Expression of Ss-caf1 without signal peptides in Nicotiana benthamiana via Tobacco rattle virus-based vectors elicited cell death. These results suggest that Ss-caf1 plays an important role in compound appressorium formation and sclerotial development of S. sclerotiorum. In addition, Ss-Caf1 has the potential to interact with certain host proteins or unknown substances in host cells, resulting in subsequent host cell death.
AbstractList	To decipher the mechanism of pathogenicity in Sclerotinia sclerotiorum, a pathogenicity-defective mutant, Sunf-MT6, was isolated from a T-DNA insertional library. Sunf-MT6 could not form compound appressorium and failed to induce lesions on leaves of rapeseed though it could produce more oxalic acid than the wild-type strain. However, it could enter into host tissues via wounds and cause typical necrotic lesions. Furthermore, Sunf-MT6 produced fewer but larger sclerotia than the wild-type strain Sunf-M. A gene, named Ss-caf1, was disrupted by T-DNA insertion in Sunf-MT6. Gene complementation and knockdown experiments confirmed that the disruption of Ss-caf1 was responsible for the phenotypic changes of Sunf-MT6. Ss-caf1 encodes a secretory protein with a putative Ca(2+)-binding EF-hand motif. High expression levels of Ss-caf1 were observed at an early stage of compound appressorium formation and in immature sclerotia. Expression of Ss-caf1 without signal peptides in Nicotiana benthamiana via Tobacco rattle virus-based vectors elicited cell death. These results suggest that Ss-caf1 plays an important role in compound appressorium formation and sclerotial development of S. sclerotiorum. In addition, Ss-Caf1 has the potential to interact with certain host proteins or unknown substances in host cells, resulting in subsequent host cell death. To decipher the mechanism of pathogenicity in Sclerotinia sclerotiorum, a pathogenicity-defective mutant, Sunf-MT6, was isolated from a T-DNA insertional library. Sunf-MT6 could not form compound appressorium and failed to induce lesions on leaves of rapeseed though it could produce more oxalic acid than the wild-type strain. However, it could enter into host tissues via wounds and cause typical necrotic lesions. Furthermore, Sunf-MT6 produced fewer but larger sclerotia than the wild-type strain Sunf-M. A gene, named Ss-caf1, was disrupted by T-DNA insertion in Sunf-MT6. Gene complementation and knockdown experiments confirmed that the disruption of Ss-caf1 was responsible for the phenotypic changes of Sunf-MT6. Ss-caf1 encodes a secretory protein with a putative Ca2+-binding EF-hand motif. High expression levels of Ss-caf1 were observed at an early stage of compound appressorium formation and in immature sclerotia. Expression of Ss-caf1 without signal peptides in Nicotiana benthamiana via Tobacco rattle virus-based vectors elicited cell death. These results suggest that Ss-caf1 plays an important role in compound appressorium formation and sclerotial development of S. sclerotiorum. In addition, Ss-Caf1 has the potential to interact with certain host proteins or unknown substances in host cells, resulting in subsequent host cell death. To decipher the mechanism of pathogenicity in Sclerotinia sclerotiorum, a pathogenicity-defective mutant, Sunf-MT6, was isolated from a T-DNA insertional library. Sunf-MT6 could not form compound appressorium and failed to induce lesions on leaves of rapeseed though it could produce more oxalic acid than the wild-type strain. However, it could enter into host tissues via wounds and cause typical necrotic lesions. Furthermore, Sunf-MT6 produced fewer but larger sclerotia than the wild-type strain Sunf-M. A gene, named Ss-caf1, was disrupted by T-DNA insertion in Sunf-MT6. Gene complementation and knockdown experiments confirmed that the disruption of Ss-caf1 was responsible for the phenotypic changes of Sunf-MT6. Ss-caf1 encodes a secretory protein with a putative Ca(2+)-binding EF-hand motif. High expression levels of Ss-caf1 were observed at an early stage of compound appressorium formation and in immature sclerotia. Expression of Ss-caf1 without signal peptides in Nicotiana benthamiana via Tobacco rattle virus-based vectors elicited cell death. These results suggest that Ss-caf1 plays an important role in compound appressorium formation and sclerotial development of S. sclerotiorum. In addition, Ss-Caf1 has the potential to interact with certain host proteins or unknown substances in host cells, resulting in subsequent host cell death.To decipher the mechanism of pathogenicity in Sclerotinia sclerotiorum, a pathogenicity-defective mutant, Sunf-MT6, was isolated from a T-DNA insertional library. Sunf-MT6 could not form compound appressorium and failed to induce lesions on leaves of rapeseed though it could produce more oxalic acid than the wild-type strain. However, it could enter into host tissues via wounds and cause typical necrotic lesions. Furthermore, Sunf-MT6 produced fewer but larger sclerotia than the wild-type strain Sunf-M. A gene, named Ss-caf1, was disrupted by T-DNA insertion in Sunf-MT6. Gene complementation and knockdown experiments confirmed that the disruption of Ss-caf1 was responsible for the phenotypic changes of Sunf-MT6. Ss-caf1 encodes a secretory protein with a putative Ca(2+)-binding EF-hand motif. High expression levels of Ss-caf1 were observed at an early stage of compound appressorium formation and in immature sclerotia. Expression of Ss-caf1 without signal peptides in Nicotiana benthamiana via Tobacco rattle virus-based vectors elicited cell death. These results suggest that Ss-caf1 plays an important role in compound appressorium formation and sclerotial development of S. sclerotiorum. In addition, Ss-Caf1 has the potential to interact with certain host proteins or unknown substances in host cells, resulting in subsequent host cell death.
Author	Fu, Yanping Yi, Xianhong Cheng, Jiasen Xie, Jiatao Jiang, Daohong Xiao, Xueqiong Li, Guoqing
Author_xml	– sequence: 1 givenname: Xueqiong surname: Xiao fullname: Xiao, Xueqiong – sequence: 2 givenname: Jiatao surname: Xie fullname: Xie, Jiatao – sequence: 3 givenname: Jiasen surname: Cheng fullname: Cheng, Jiasen – sequence: 4 givenname: Guoqing surname: Li fullname: Li, Guoqing – sequence: 5 givenname: Xianhong surname: Yi fullname: Yi, Xianhong – sequence: 6 givenname: Daohong surname: Jiang fullname: Jiang, Daohong – sequence: 7 givenname: Yanping surname: Fu fullname: Fu, Yanping
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/24299212$$D View this record in MEDLINE/PubMed
BookMark	eNo9UU1v1DAQtVAR3RZ-ABfkIxdTO_5IfEQraFdqAbXlHDn2ZNdVYqe2g9S_wq_FotteZp5G7715mjlDJyEGQOgjo18Y1eLi5tfNjlBJGCeUCUlu36AN04KTtqXqBG1opwWhTdecorOcHyhlWkn5Dp02otG6Yc0G_f0R_8CEM9gEJaYnvKRYwAd8l8nWjAzHEZcD4GUyoZDFlEPcQ_AWj2vYrxnf2QmqwgdvcD7imNYZ-4wTPK4-gcNjTPgQc8ELBCjJVErAJjgcYprN9Cqs0EGNE5cZQnmP3o5myvDh2M_R7-_f7rdX5Prn5W779ZpY0apCBsEpA9YYAbJRHdWDk4rBOAorgPPRDdwYaoEbqzmjSusRKGNO1apNZ_k52j37umge-iX52aSnPhrf_x_EtO9NKr5m7AcQajCiruyoAGcHVyO0yrAOpKSyq16fn73qGR9XyKWffbYw1etBXHPPhJKsVVKJSv10pK7DDO518ctv-D-RoJbf
CitedBy_id	crossref_primary_10_1111_mpp_13333 crossref_primary_10_3390_cells12071063 crossref_primary_10_3390_ijms21020603 crossref_primary_10_1007_s41348_021_00509_z crossref_primary_10_1016_j_bcab_2022_102302 crossref_primary_10_3389_fmicb_2018_02658 crossref_primary_10_1016_j_molp_2020_08_016 crossref_primary_10_3389_fmicb_2019_02228 crossref_primary_10_3389_fmicb_2016_01787 crossref_primary_10_1016_j_pmpp_2019_02_002 crossref_primary_10_1111_mpp_12801 crossref_primary_10_1111_mpp_12922 crossref_primary_10_1007_s10327_017_0755_2 crossref_primary_10_1016_j_funbio_2021_09_004 crossref_primary_10_3389_fmicb_2018_03031 crossref_primary_10_3390_jof9050520 crossref_primary_10_1038_s41467_024_53725_0 crossref_primary_10_1111_mpp_12913 crossref_primary_10_1371_journal_ppat_1005435 crossref_primary_10_1002_ps_7812 crossref_primary_10_3389_fmicb_2022_816091 crossref_primary_10_1139_cjm_2016_0091 crossref_primary_10_3389_fpls_2022_1008665 crossref_primary_10_1007_s40858_018_0259_4 crossref_primary_10_1111_mpp_13221 crossref_primary_10_1111_mpp_12453 crossref_primary_10_1111_mpp_13464 crossref_primary_10_1111_mpp_12459 crossref_primary_10_3389_fmicb_2016_01798 crossref_primary_10_1016_j_hpj_2022_05_001 crossref_primary_10_1111_mpp_13423 crossref_primary_10_1016_j_biocontrol_2015_04_013 crossref_primary_10_3389_fpls_2023_1139009 crossref_primary_10_3390_ijms26010201 crossref_primary_10_3389_fpls_2016_00422 crossref_primary_10_3390_ijms19102946 crossref_primary_10_3389_fpls_2024_1360024 crossref_primary_10_1016_j_funbio_2018_12_003 crossref_primary_10_1016_j_jprot_2016_03_006 crossref_primary_10_1371_journal_pone_0229844 crossref_primary_10_1186_s12864_020_6581_5 crossref_primary_10_1094_PHYTO_08_15_0181_R crossref_primary_10_3389_fpls_2016_00964 crossref_primary_10_1111_jam_14973 crossref_primary_10_1093_plphys_kiab274 crossref_primary_10_3390_jof8050526 crossref_primary_10_1094_PDIS_11_15_1349_RE crossref_primary_10_3389_fmicb_2018_01828 crossref_primary_10_1016_j_ibiod_2023_105648 crossref_primary_10_1094_PHYTO_06_18_0204_R crossref_primary_10_3389_fpls_2023_1096831 crossref_primary_10_1007_s00284_017_1309_8 crossref_primary_10_1007_s11557_021_01737_1 crossref_primary_10_3390_pathogens9010027 crossref_primary_10_1016_j_micpath_2023_106276 crossref_primary_10_3390_v10050254 crossref_primary_10_1128_spectrum_02612_23 crossref_primary_10_1128_microbiolspec_FUNK_0023_2016 crossref_primary_10_3389_fmicb_2016_00083 crossref_primary_10_3389_fmicb_2021_693334 crossref_primary_10_1094_PHYTO_06_18_0197_RVW crossref_primary_10_1007_s10658_025_03131_z crossref_primary_10_1016_j_pmpp_2015_04_005 crossref_primary_10_1146_annurev_phyto_080417_050052 crossref_primary_10_3389_fmicb_2024_1424130 crossref_primary_10_3390_ijms23116066 crossref_primary_10_1038_srep15565 crossref_primary_10_3389_fpls_2020_01021 crossref_primary_10_1016_j_ncrops_2024_100046 crossref_primary_10_1186_s12864_021_07655_6 crossref_primary_10_1094_PDIS_03_16_0403_RE crossref_primary_10_1186_s12864_017_3642_5 crossref_primary_10_1111_nph_14842 crossref_primary_10_1080_15427528_2022_2120145 crossref_primary_10_1007_s11033_024_09314_x crossref_primary_10_1016_j_cj_2024_01_016
ContentType	Journal Article
DBID	CGR CUY CVF ECM EIF NPM 7X8 DOA
DOI	10.1094/MPMI-05-13-0145-R
DatabaseName	Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic DOAJ Directory of Open Access Journals
DatabaseTitle	MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic
DatabaseTitleList	MEDLINE MEDLINE - Academic
Database_xml	– sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Agriculture Biology
EISSN	1943-7706
EndPage	55
ExternalDocumentID	oai_doaj_org_article_be46ba4b43804edcbd47676a18e55058 24299212
Genre	Research Support, Non-U.S. Gov't Journal Article
GroupedDBID	--- 123 29M 2WC 53G 7X2 7X7 88E 8AO 8CJ 8FE 8FH 8FI 8FJ 8FW 8R4 8R5 AAHBH AAYJJ ABDNZ ABRJW ABUWG ACGFO ACPRK ACYGS ADBBV AENEX AEUYN AFKRA AFRAH ALIPV ALMA_UNASSIGNED_HOLDINGS ATCPS BAWUL BBNVY BENPR BES BHPHI BPHCQ BVXVI C1A CCPQU CGR CS3 CUY CVF D1J DIK DU5 E3Z EBS ECM EIF EJD F5P FRP FYUFA GROUPED_DOAJ HCIFZ HMCUK HYO LK8 M0K M1P M7P MVM NPM OK1 P2P PHGZT PQQKQ PROAC PSQYO Q2X RPS S0X TR2 UKHRP YCJ ~KM 7X8
ID	FETCH-LOGICAL-c476t-b4301e12a4e526809bd561eff4c4e33fdb3aa0ce3ac9310699fe011d6e019a8c3
IEDL.DBID	DOA
ISICitedReferencesCount	86
ISICitedReferencesURI	http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000328661800004&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN	0894-0282
IngestDate	Fri Oct 03 12:44:58 EDT 2025 Thu Sep 04 17:10:41 EDT 2025 Thu Apr 03 07:04:44 EDT 2025
IsDoiOpenAccess	true
IsOpenAccess	true
IsPeerReviewed	true
IsScholarly	true
Issue	1
Language	English
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c476t-b4301e12a4e526809bd561eff4c4e33fdb3aa0ce3ac9310699fe011d6e019a8c3
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
OpenAccessLink	https://doaj.org/article/be46ba4b43804edcbd47676a18e55058
PMID	24299212
PQID	1465176564
PQPubID	23479
PageCount	16
ParticipantIDs	doaj_primary_oai_doaj_org_article_be46ba4b43804edcbd47676a18e55058 proquest_miscellaneous_1465176564 pubmed_primary_24299212
PublicationCentury	2000
PublicationDate	2014-Jan 20140101 2014-01-01
PublicationDateYYYYMMDD	2014-01-01
PublicationDate_xml	– month: 01 year: 2014 text: 2014-Jan
PublicationDecade	2010
PublicationPlace	United States
PublicationPlace_xml	– name: United States
PublicationTitle	Molecular plant-microbe interactions
PublicationTitleAlternate	Mol Plant Microbe Interact
PublicationYear	2014
Publisher	The American Phytopathological Society
Publisher_xml	– name: The American Phytopathological Society
SSID	ssj0019655
Score	2.4046626
Snippet	To decipher the mechanism of pathogenicity in Sclerotinia sclerotiorum, a pathogenicity-defective mutant, Sunf-MT6, was isolated from a T-DNA insertional...
SourceID	doaj proquest pubmed
SourceType	Open Website Aggregation Database Index Database
StartPage	40
SubjectTerms	Amino Acid Motifs Arabidopsis - microbiology Arabidopsis - physiology Ascomycota - genetics Ascomycota - pathogenicity Ascomycota - physiology Ascomycota - ultrastructure Cell Death Daucus carota - microbiology Daucus carota - physiology Fruiting Bodies, Fungal Fungal Proteins - genetics Fungal Proteins - metabolism Gene Library Gene Silencing Host-Pathogen Interactions Models, Molecular Mutagenesis, Insertional Nicotiana - microbiology Nicotiana - physiology Oxalic Acid - metabolism Phenotype Phylogeny Plant Diseases - microbiology Plant Leaves - microbiology Plant Leaves - physiology Plant Roots - microbiology Plant Roots - physiology Seedlings - microbiology Seedlings - physiology Stress, Physiological
Title	Novel secretory protein Ss-Caf1 of the plant-pathogenic fungus Sclerotinia sclerotiorum is required for host penetration and normal sclerotial development
URI	https://www.ncbi.nlm.nih.gov/pubmed/24299212 https://www.proquest.com/docview/1465176564 https://doaj.org/article/be46ba4b43804edcbd47676a18e55058
Volume	27
WOSCitedRecordID	wos000328661800004&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1La9wwEBZtaKE5lDR9ZNsmqNCriL3W6nFMQ5cEusZk27I3I0ujYgh2WXsD_Sv9tZ2xvSGX0ksvQhhsiRlJ841m_A1jHw2adQ1xLigGJGSKurALk4qgnYpoH40fsgm_f9F5bjYbWzwo9UU5YSM98Ci48wqkqpysiBldQvBVkFpp5VIDBK6H33wTbffO1BQ_sGqod5oYIr5Fr2Ifz7TyfFWsrin8S1UNUrkQNxNb_98h5mBqlkfs-YQR-cU4txfsETTH7PDix3biyYBj9nSsIfnrJfudt3dwy9cE_yhgzgtiXqgbvu7EpYspbyNHkMepOlEvCgR8La6Z2vMlbvNdx9c4BL5RN7Xj3dQnggZ-3fEboDxhCByRLb9qu54XeDRORLvcNYHnBHlv7z-C3QdZSK_Yt-Xnr5dXYiq4IDwKtRco5CSFdO4kEAtMYquA8ApilF5ClsVQZc4lHjLnLcJCZW0EPB-CwtY647PX7KBpGzhhHJKFr9AAow8cpHHOeuUg0_MsSgkqwIx9IqGXP0dOjZJYrocHqPty0n35L93P2Ie9ykrcFRTqcA20u44cmkWqEavKGXsz6vJ-qDmZYLTYb__HFN6xZwih5Hgp854d9NsdnLIn_q6vu-0Ze6w35mxYmtjmxeoPRZvpIw
linkProvider	Directory of Open Access Journals
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Novel+Secretory+Protein+Ss-Caf1+of+the+Plant-Pathogenic+Fungus+Sclerotinia+sclerotiorum+Is+Required+for+Host+Penetration+and+Normal+Sclerotial+Development&rft.jtitle=Molecular+plant-microbe+interactions&rft.au=Xueqiong+Xiao&rft.au=Jiatao+Xie&rft.au=Jiasen+Cheng&rft.au=Guoqing+Li&rft.date=2014-01-01&rft.pub=The+American+Phytopathological+Society&rft.issn=0894-0282&rft.eissn=1943-7706&rft.volume=27&rft.issue=1&rft.spage=40&rft.epage=55&rft_id=info:doi/10.1094%2FMPMI-05-13-0145-R&rft.externalDBID=DOA&rft.externalDocID=oai_doaj_org_article_be46ba4b43804edcbd47676a18e55058
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0894-0282&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0894-0282&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0894-0282&client=summon