Classification and characterization of a laboratory chicken rotavirus strain carrying G7P[35] neutralization antigens on the genotype 4 backbone gene configuration

The laboratory rotavirus strain, BRS/115, has been used for more than two decades to monitor rotaviruses in specific pathogen free flocks of laying hens. However, the virus strain has not been characterized in detail. Therefore we aimed at the description of molecular features of BRS115 by using ran...

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Bibliographic Details
Published in:Biologicals Vol. 42; no. 6; pp. 299 - 304
Main Authors: Papp, Hajnalka, Marton, Szilvia, Farkas, Szilvia L., Jakab, Ferenc, Martella, Vito, Malik, Yashpal S., Palya, Vilmos, Bányai, Krisztián
Format: Journal Article
Language:English
Published: England Elsevier Ltd 01.11.2014
Subjects:
Animals
antigens
Chickens - virology
DNA, Complementary - metabolism
flocks
Gene Library
genes
genetic databases
Genome, Viral
Genotype
genotyping
guidelines
High-Throughput Nucleotide Sequencing
laying hens
neutralization
Phylogeny
pigeons
Reverse Transcriptase Polymerase Chain Reaction
RNA
RNA - genetics
Rotavirus
Rotavirus - genetics
Rotavirus A
Semiconductors
Sequence Analysis, DNA
specific pathogen-free animals
transcription (genetics)
viruses
Genotype
Rotavirus A
Phylogeny
ISSN:1045-1056, 1095-8320
Online Access:Get full text
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Summary:The laboratory rotavirus strain, BRS/115, has been used for more than two decades to monitor rotaviruses in specific pathogen free flocks of laying hens. However, the virus strain has not been characterized in detail. Therefore we aimed at the description of molecular features of BRS115 by using random primed reverse transcription-PCR of the genomic RNA followed by massive parallel sequencing using the semiconductor sequencing technology. Over 64,000 trimmed reads mapped to reference sequences obtained from GenBank. The strain classified into the species Rotavirus A and genotyped G7-P[35]-I4-R4-C4-M4-A16-N4-T4-E11-H4 according to guidelines of the Rotavirus Classification Working Group. Phylogenetic analysis identified shared features with chicken, turkey and pigeon origin rotaviruses. This study demonstrates the robustness of next generation sequencing in the characterization of reference virus materials used in specialized laboratories.
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ISSN:1045-1056
1095-8320
DOI:10.1016/j.biologicals.2014.08.004