An AIE‐Based Probe for Rapid and Ultrasensitive Imaging of Plasma Membranes in Biosystems

The abnormality of the plasma membrane (PM) is an important biomarker for cell status and many diseases. Hence, visualizing the PM, especially in complex systems, is an emerging field in the life sciences, especially in low‐resource settings. Herein, we developed a water‐soluble PM‐specific probe ut...

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Veröffentlicht in:Angewandte Chemie International Edition Jg. 59; H. 25; S. 9962 - 9966
Hauptverfasser: Shi, Lei, Liu, Yan‐Hong, Li, Kun, Sharma, Amit, Yu, Kang‐Kang, Ji, Myung Sun, Li, Ling‐Ling, Zhou, Qian, Zhang, Hong, Kim, Jong Seung, Yu, Xiao‐Qi
Format: Journal Article
Sprache:Englisch
Veröffentlicht: WEINHEIM Wiley 15.06.2020
Wiley Subscription Services, Inc
Ausgabe:International ed. in English
Schlagworte:
aggregation-induced emission
Animals
Biocompatibility
Biocompatible Materials
Biomarkers
Cell Membrane - ultrastructure
Chemistry
Chemistry, Multidisciplinary
Complex systems
Electrostatic properties
Epidermis - ultrastructure
Erythrocytes
Erythrocytes - ultrastructure
Eye - ultrastructure
Fluorescence
Fluorescent Dyes - chemistry
fluorescent probes
Humans
Hydrophobicity
Medical imaging
Membranes
Neuroimaging
neurons
Neurons - ultrastructure
Optical Imaging
Physical Sciences
Plasma membranes
Science & Technology
Spectrometry, Fluorescence
structural biology
Zebrafish
plasma membranes
TRACKING
aggregation-induced emission
structural biology
GENERATION
neurons
fluorescent probes
ISSN:1433-7851, 1521-3773, 1521-3773
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Zusammenfassung:The abnormality of the plasma membrane (PM) is an important biomarker for cell status and many diseases. Hence, visualizing the PM, especially in complex systems, is an emerging field in the life sciences, especially in low‐resource settings. Herein, we developed a water‐soluble PM‐specific probe utilizing electrostatic and hydrophobic interaction strategies with aggregation‐induced emission as the signal output. The probe could image the PM with many advanced features (wash‐free, ultrafast staining process, excellent PM specificity, and good biocompatibility), which were demonstrated by the PM imaging of neurons. The probe allowed for the first time the imaging of erythrocytes in the complex brain environment through a fluorescence‐based method. Moreover, the PM of the epidermal and partial view of the eyeball structure of live zebrafish are also revealed. Insane in the membrane: A plasma membrane (PM)‐specific probe with aggregation‐induced emission characteristics for wash‐free PM imaging is presented. This is the first time that erythrocytes have been visualized in the brain through a fluorescence‐based method. Moreover, a partial view of the eyeball structure of live zebrafish was obtained through the in situ labelling of the epidermal PM.
Bibliographie:ObjectType-Article-1
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ISSN:1433-7851
1521-3773
1521-3773
DOI:10.1002/anie.201909498