Measuring the sequence-affinity landscape of antibodies with massively parallel titration curves

Despite the central role that antibodies play in the adaptive immune system and in biotechnology, much remains unknown about the quantitative relationship between an antibody’s amino acid sequence and its antigen binding affinity. Here we describe a new experimental approach, called Tite-Seq, that i...

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Bibliographic Details
Published in:	eLife Vol. 5
Main Authors:	Adams, Rhys M, Mora, Thierry, Walczak, Aleksandra M, Kinney, Justin B
Format:	Journal Article
Language:	English
Published:	England eLife Sciences Publications Ltd 30.12.2016 eLife Sciences Publications, Ltd
Subjects:	Affinity Amino Acid Sequence Antibodies antibody Antibody Affinity Antibody Specificity Antigen-Antibody Complex - analysis Antigens Binding Sites, Antibody Biophysics and Structural Biology Biotechnology deep mutational scan dissociation constant Experiments Flow cytometry High-Throughput Screening Assays Humans Immune system Immunoglobulins Kinetics Ligands Models, Molecular Mutation Oligonucleotides - chemistry Oligonucleotides - genetics Oligonucleotides - metabolism Peptide Library Protein Binding Protein expression Proteins Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Single-Chain Antibodies - chemistry Single-Chain Antibodies - genetics Single-Chain Antibodies - immunology Structure-Activity Relationship Titration titration curve Yeast titration curve antibody deep mutational scan dissociation constant structural biology biophysics S. cerevisiae affinity
ISSN:	2050-084X, 2050-084X
Online Access:	Get full text
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