X-CHIME enables combinatorial, inducible, lineage-specific and sequential knockout of genes in the immune system

Annotation of immunologic gene function in vivo typically requires the generation of knockout mice, which is time consuming and low throughput. We previously developed CHimeric IMmune Editing (CHIME), a CRISPR–Cas9 bone marrow delivery system for constitutive, ubiquitous deletion of single genes. He...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	Nature immunology Ročník 25; číslo 1; s. 178 - 188
Hlavní autoři:	LaFleur, Martin W., Lemmen, Ashlyn M., Streeter, Ivy S. L., Nguyen, Thao H., Milling, Lauren E., Derosia, Nicole M., Hoffman, Zachary M., Gillis, Jacob E., Tjokrosurjo, Qin, Markson, Samuel C., Huang, Amy Y., Anekal, Praju V., Montero Llopis, Paula, Haining, W. Nicholas, Doench, John G., Sharpe, Arlene H.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	New York Nature Publishing Group US 01.01.2024 Nature Publishing Group
Témata:	631/250/232/1997 631/250/2502/248 Animals Biomedical and Life Sciences Biomedicine Bone marrow CRISPR CRISPR-Cas Systems - genetics Enteritis Gene deletion Gene Editing Genes Hematopoietic stem cells Hypoplasia Immune System Immunology Infectious Diseases Lethality Mice Mice, Knockout Protein Tyrosine Phosphatase, Non-Receptor Type 2 - genetics PTPN2 protein technical-report
ISSN:	1529-2908, 1529-2916, 1529-2916
On-line přístup:	Získat plný text
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Buďte první, kdo okomentuje tento záznam!