Novel Antioxidant, Anti-α-Amylase, Anti-Inflammatory and Antinociceptive Water-Soluble Polysaccharides from the Aerial Part of Nitraria retusa

In this paper, water-soluble polysaccharides (named as NRLP) were extracted from Nitraria retusa leaves. The main structural features of NRLP were determined by High-pressure size exclusion chromatography, Fourier transform infrared and Gas Chromatography/Mass Spectrometry-Electronic Impact analysis...

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Bibliographic Details
Published in:Foods Vol. 9; no. 1; p. 28
Main Authors: Rjeibi, Ilhem, Hentati, Faiez, Feriani, Anouar, Hfaiedh, Najla, Delattre, Cédric, Michaud, Philippe, Pierre, Guillaume
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 26.12.2019
MDPI
Subjects:
aerial parts
Amylases
antioxidant activity
Antioxidants
Biochemistry
Biochemistry, Molecular Biology
biological activities
Chemical and Process Engineering
Chromatography
Chronic fatigue syndrome
dose response
Engineering Sciences
Ethanol
Feature extraction
Food science
Fourier analysis
Fourier transform infrared spectroscopy
Fourier transforms
Gas chromatography
gel chromatography
Impact analysis
Inflammation
Inflammatory diseases
Infrared analysis
Life Sciences
Mass spectrometry
Mass spectroscopy
mice
Molecular weight
Nitraria
Nitraria retusa
Oxidative stress
Pain perception
physicochemical characterization
Polydispersity
Polysaccharides
Saccharides
Scavenging
Size exclusion chromatography
Water chemistry
water solubility
α-Amylase
United States > US
Tunisia
Germany
polysaccharides
Nitraria retusa
physicochemical characterization
biological activities
mice
ISSN:2304-8158, 2304-8158
Online Access:Get full text
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Summary:In this paper, water-soluble polysaccharides (named as NRLP) were extracted from Nitraria retusa leaves. The main structural features of NRLP were determined by High-pressure size exclusion chromatography, Fourier transform infrared and Gas Chromatography/Mass Spectrometry-Electronic Impact analysis. The in vitro and in vivo biological potential of NRLP were evaluated by measuring its antioxidant (•OH and DPPH• scavenging, total antioxidant capacity), anti-α-amylase as well as anti-inflammatory and antinociceptive activities in a mice model. NRLP was composed of Rha (33.7%), Gal (18.1%), GalA (15.0%), Glc (13.3%), Ara (13.3%), Xyl (3.8%), and GlcA (2.8%) and showed a Molecular Weight (Mw) of 23.0 kDa and a polydispersity index (PDI) of 1.66. The investigations highlighted a significant antioxidant activity (IC50 = 2.4–2.6 mg/mL) and an inhibition activity against α-amylase (IC50 = 4.55 mg/mL) in a dose-dependent manner. Further, NRLP revealed interesting anti-edematous effects and antinociceptive activities (both > 70%). These results open up new pharmacological prospects for the water-soluble polysaccharides extracted from Nitraria retusa leaves.
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ISSN:2304-8158
2304-8158
DOI:10.3390/foods9010028