A Mechanism for Controlled Breakage of Under-replicated Chromosomes during Mitosis

While DNA replication and mitosis occur in a sequential manner, precisely how cells maintain their temporal separation and order remains elusive. Here, we unveil a double-negative feedback loop between replication intermediates and an M-phase-specific structure-selective endonuclease, MUS81-SLX4, wh...

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Veröffentlicht in:Developmental cell Jg. 39; H. 6; S. 740 - 755
Hauptverfasser: Duda, Heike, Arter, Meret, Gloggnitzer, Jiradet, Teloni, Federico, Wild, Philipp, Blanco, Miguel G, Altmeyer, Matthias, Matos, Joao
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 19.12.2016
Schlagworte:
CDC2 Protein Kinase - metabolism
Cell Cycle Proteins - metabolism
Cell Death
Cell Survival
Chromosomes, Human - metabolism
DNA Damage
DNA Fragmentation
DNA Replication
DNA-Binding Proteins - metabolism
Endonucleases - metabolism
HeLa Cells
Humans
Mitosis
Models, Biological
Phosphorylation
Protein Binding
Recombinases - metabolism
S Phase
MUS81
DNA replication
WEE1
SLX4
EME2
cell cycle
PLK1
chromosome pulverization
CDK1
ISSN:1878-1551
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Zusammenfassung:While DNA replication and mitosis occur in a sequential manner, precisely how cells maintain their temporal separation and order remains elusive. Here, we unveil a double-negative feedback loop between replication intermediates and an M-phase-specific structure-selective endonuclease, MUS81-SLX4, which renders DNA replication and mitosis mutually exclusive. MUS81 nuclease is constitutively active throughout the cell cycle but requires association with SLX4 for efficient substrate targeting. To preclude toxic processing of replicating chromosomes, WEE1 kinase restrains CDK1 and PLK1-mediated MUS81-SLX4 assembly during S phase. Accordingly, WEE1 inhibition triggers widespread nucleolytic breakage of replication intermediates, halting DNA replication and leading to chromosome pulverization. Unexpectedly, premature entry into mitosis-licensed by unrestrained CDK1 activity during S phase-requires MUS81-SLX4, which inhibits DNA replication. This suggests that ongoing replication assists WEE1 in delaying entry into M phase and, indirectly, in preventing MUS81-SLX4 assembly. Conversely, MUS81-SLX4 activation during mitosis promotes targeted resolution of persistent replication intermediates, which safeguards chromosome segregation.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1878-1551
DOI:10.1016/j.devcel.2016.11.017