The development of a novel diagnostic PCR for Madurella mycetomatis using a comparative genome approach

Eumycetoma is a neglected tropical disease most commonly caused by the fungus Madurella mycetomatis. Identification of eumycetoma causative agents can only be reliably performed by molecular identification, most commonly by species-specific PCR. The current M. mycetomatis specific PCR primers were r...

Full description

Saved in:
Bibliographic Details
Published in:PLoS neglected tropical diseases Vol. 14; no. 12; p. e0008897
Main Authors: Lim, Wilson, Siddig, Emmanuel, Eadie, Kimberly, Nyuykonge, Bertrand, Ahmed, Sarah, Fahal, Ahmed, Verbon, Annelies, Smit, Sandra, van de Sande, Wendy WJ
Format: Journal Article
Language:English
Published: United States Public Library of Science 01.12.2020
Public Library of Science (PLoS)
Subjects:
Biology and Life Sciences
Cross Reactions
Fungal Proteins
Humans
Madurella
Medicine and Health Sciences
Mycetoma - diagnosis
Mycetoma - microbiology
Neglected Diseases - diagnosis
Neglected Diseases - microbiology
Polymerase Chain Reaction - methods
Research and Analysis Methods
Species Specificity
ISSN:1935-2735, 1935-2727, 1935-2735
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Eumycetoma is a neglected tropical disease most commonly caused by the fungus Madurella mycetomatis. Identification of eumycetoma causative agents can only be reliably performed by molecular identification, most commonly by species-specific PCR. The current M. mycetomatis specific PCR primers were recently discovered to cross-react with Madurella pseudomycetomatis. Here, we used a comparative genome approach to develop a new M. mycetomatis specific PCR for species identification. Predicted-protein coding sequences unique to M. mycetomatis were first identified in BLASTCLUST based on E-value, size and presence of orthologues. Primers were then developed for 16 unique sequences and evaluated against 60 M. mycetomatis isolates and other eumycetoma causing agents including the Madurella sibling species. Out of the 16, only one was found to be specific to M. mycetomatis. We have discovered a predicted-protein coding sequence unique to M. mycetomatis and have developed a new species-specific PCR to be used as a novel diagnostic marker for M. mycetomatis.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Current address: Faculty of medical laboratory sciences, University of Khartoum, Sudan
Current address: Center of Expertise in Mycology of Radboud University Medical Center / Canisius Wilhelmina Hospital, Nijmegen, The Netherlands
The authors have declared that no competing interests exist.
ISSN:1935-2735
1935-2727
1935-2735
DOI:10.1371/journal.pntd.0008897