Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil

We identified a novel plant rhabdovirus infecting native joá ( Solanum aculeatissimum ) plants in Brazil. Infected plants showed yellow blotches on the leaves, and typical enveloped bacilliform rhabdovirus particles associated with the nucleus were seen in thin sections by electron microscopy. The v...

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Vydáno v:	Archives of virology Ročník 166; číslo 6; s. 1615 - 1622
Hlavní autoři:	Dietzgen, Ralf G., Bejerman, Nicolas E., Mei, Yongyu, Jee, Charmaine Lim Jing, Chabi-Jesus, Camila, Freitas-Astúa, Juliana, Veras, Solange M., Kitajima, Elliot W.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Vienna Springer Vienna 01.06.2021 Springer Nature B.V
Témata:	Biomedical and Life Sciences Biomedicine Blotch Brazil Disease transmission Dwarf disease Electron microscopy Flowers & plants Gene mapping genes Genomes Infectious Diseases L gene L protein Medical Microbiology New species Nucleotide sequence Nucleotides Original Article Phylogeny Polymerase chain reaction Potato yellow dwarf virus Rhabdoviridae RNA Solanum aculeatissimum tomatoes Virology Viruses Brazil
ISSN:	0304-8608, 1432-8798, 1432-8798
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Shrnutí:	We identified a novel plant rhabdovirus infecting native joá ( Solanum aculeatissimum ) plants in Brazil. Infected plants showed yellow blotches on the leaves, and typical enveloped bacilliform rhabdovirus particles associated with the nucleus were seen in thin sections by electron microscopy. The virus could be graft-transmitted to healthy joá and tomato plants but was not mechanically transmissible. RT-PCR using degenerate plant rhabdovirus L gene primers yielded an amplicon from extracted total RNA, the sequence of which was similar to those of alphanucleorhabdoviruses. Based on close sequence matches, especially with the type member potato yellow dwarf virus (PYDV), we adopted a degenerate-primer-walking strategy towards both genome ends. The complete genome of joá yellow blotch-associated virus (JYBaV) is comprised of 12,965 nucleotides, is less than 75% identical to that of its closest relative PYDV, and clusters with PYDV and other alphanucleorhabdoviruses in L protein phylogenetic trees, suggesting that it should be taxonomically classified in a new species in the genus Alphanucleorhabdovirus , family Rhabdoviridae . The genome organization of JYBaV is typical of the ‘PYDV-like’ subgroup of alphanucleorhabdoviruses, with seven genes ( N-X-P-Y-M-G-L ) separated by conserved intergenic regions and flanked by partly complementary 3' leader and 5' trailer regions.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	0304-8608 1432-8798 1432-8798
DOI:	10.1007/s00705-021-05040-y