Solution NMR backbone assignments of disordered Olduvai protein domain CON1 employing Hα-detected experiments

Olduvai protein domains, encoded by the NBPF gene family, are responsible for the largest increase in copy number of any protein-coding region in the human genome. This has spawned various genetics studies which have linked these domains to human brain development and divergence from our primate anc...

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Vydáno v:	Biomolecular NMR assignments Ročník 16; číslo 1; s. 113 - 119
Hlavní autoři:	Paukovich, Natasia, Henen, Morkos A., Hussain, Alya, Issaian, Aaron, Sikela, James M., Hansen, Kirk C., Vögeli, Beat
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Dordrecht Springer Netherlands 01.04.2022 Springer Nature B.V
Témata:	Animals Autism Autism Spectrum Disorder - genetics Biochemistry Biological and Medical Physics Biophysics Cognitive ability Copy number Experiments Genome, Human Genomes Humans Mental disorders Neural coding NMR Nuclear magnetic resonance Nuclear Magnetic Resonance, Biomolecular Physics Physics and Astronomy Polymer Sciences Protein Domains - genetics Proteins Schizophrenia DUF1220 Autism IDP Olduvai domain Backbone chemical shift assignment
ISSN:	1874-2718, 1874-270X, 1874-270X
On-line přístup:	Získat plný text
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Shrnutí:	Olduvai protein domains, encoded by the NBPF gene family, are responsible for the largest increase in copy number of any protein-coding region in the human genome. This has spawned various genetics studies which have linked these domains to human brain development and divergence from our primate ancestors, as well as currently relevant cognitive diseases such as schizophrenia and autism spectrum disorder (ASD). There are six separate Olduvai domains which together form the majority of the various protein products of the NBPF genes. The six domains include three conserved domains (CON1-3), and three human-lineage-specific domains (HLS1-3) which occur in triplet. Here, we present the solution nuclear magnetic resonance backbone assignments for the CON1 domain, which has been linked to the severity of ASD. The data confirm that CON1 is an intrinsically disordered protein (IDP). Additionally, we use innovative H α -detected experiments which allow us to not only assign the H α atoms and N atoms of proline residues, but also to assign residues where H N -experiments suffered from peak overlap or broadening.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	1874-2718 1874-270X 1874-270X
DOI:	10.1007/s12104-022-10068-5