Development of 16S rRNA-based probes for the Coriobacterium group and the Atopobium cluster and their application for enumeration of Coriobacteriaceae in human feces from volunteers of different age groups

Two 16S rRNA-targeted probes were developed: one for the Coriobacterium group and the other for the Atopobium cluster (which comprises most of the Coriobacteriaceae species, including the Coriobacterium group). The new probes were based on sequences of three new Coriobacteriaceae strains isolated fr...

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Bibliographic Details
Published in:Applied and environmental microbiology Vol. 66; no. 10; p. 4523
Main Authors: Harmsen, H J, Wildeboer-Veloo, A C, Grijpstra, J, Knol, J, Degener, J E, Welling, G W
Format: Journal Article
Language:English
Published: United States 01.10.2000
Subjects:
Actinobacteria - classification
Actinobacteria - genetics
Actinobacteria - isolation & purification
Adult
Aged
Aged, 80 and over
Aging
Breast Feeding
Child
Child, Preschool
Feces - microbiology
Humans
In Situ Hybridization, Fluorescence
Infant
Infant Food
Infant, Newborn
Middle Aged
Milk, Human
Molecular Sequence Data
Phenotype
Phylogeny
RNA, Ribosomal, 16S - genetics
ISSN:0099-2240
Online Access:Get more information
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Summary:Two 16S rRNA-targeted probes were developed: one for the Coriobacterium group and the other for the Atopobium cluster (which comprises most of the Coriobacteriaceae species, including the Coriobacterium group). The new probes were based on sequences of three new Coriobacteriaceae strains isolated from human feces and clinical material and sequences from databases. Application of the probes to fecal samples showed that formula-fed infants had higher numbers of Coriobacterium group cells in their feces than breast-fed infants. In addition, based on the presented results, it is hypothesized that with the increasing age of a person, the diversity of Atopobium cluster species present in the feces increases.
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ISSN:0099-2240
DOI:10.1128/AEM.66.10.4523-4527.2000