Evaluation of Xpert MTB/RIF assay for rapid molecular diagnosis of tuberculosis in a two-year period in Croatia

Abstract Mycobacterium tuberculosis remains a major global health problem and is currently killing 1.5 million people every year. One of the most important steps in tuberculosis control is the rapid and accurate laboratory diagnosis. The Xpert MTB/RIF assay is a novel molecular, easy-to-use assay, w...

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Veröffentlicht in:International journal of mycobacteriology Jg. 2; H. 3; S. 179 - 182
Hauptverfasser: Zmak, Ljiljana, Jankovic, Mateja, Jankovic, Vera Katalinic
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Netherlands Wolters Kluwer Medknow Publications 01.09.2013
Schlagworte:
Infectious Disease
Medical Education
Molecular methods
Rapid diagnosis
Tuberculosis
Xpert MTB/RIF assay
Rapid diagnosis
Tuberculosis
Molecular methods
Xpert MTB/RIF assay
ISSN:2212-5531, 2212-554X
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Zusammenfassung:Abstract Mycobacterium tuberculosis remains a major global health problem and is currently killing 1.5 million people every year. One of the most important steps in tuberculosis control is the rapid and accurate laboratory diagnosis. The Xpert MTB/RIF assay is a novel molecular, easy-to-use assay, which can lead to tuberculosis identification in less than 2 h. In this study, the Xpert MTB/RIF assay performance for rapid diagnosis of tuberculosis was evaluated in comparison with conventional culture methods; 361 pulmonary and extrapulmonary patient samples were collected between October 2010 and October 2012 and were analyzed at the National Reference laboratory for Mycobacteria, Zagreb, Croatia. For pulmonary samples the sensitivity and specificity were 86% and 100%, while for extrapulmonary samples the sensitivity and specificity were 75% and 99%, respectively. It was concluded that Xpert MTB/RIF assay has high sensitivity and specificity for both pulmonary and extrapulmonary specimens.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:2212-5531
2212-554X
DOI:10.1016/j.ijmyco.2013.05.003