Solution NMR backbone assignment reveals interaction-free tumbling of human lineage-specific Olduvai protein domains

Olduvai protein domains, encoded primarily by NBPF genes, have been linked to both human brain evolution and cognitive diseases such as autism and schizophrenia. There are six primary domains that comprise the Olduvai family: three conserved domains (CON1-3) and three human lineage-specific domains...

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Bibliographic Details
Published in:Biomolecular NMR assignments Vol. 13; no. 2; pp. 339 - 343
Main Authors: Issaian, Aaron, Schmitt, Lauren, Born, Alexandra, Nichols, Parker J., Sikela, James, Hansen, Kirk, Vögeli, Beat, Henen, Morkos A.
Format: Journal Article
Language:English
Published: Dordrecht Springer Netherlands 01.10.2019
Springer Nature B.V
Subjects:
Autism
Biochemistry
Biological and Medical Physics
Biophysics
Cognitive ability
Evolutionary genetics
Humans
Intracellular Signaling Peptides and Proteins - chemistry
Mental disorders
NMR
Nuclear magnetic resonance
Nuclear Magnetic Resonance, Biomolecular
Physics
Physics and Astronomy
Polymer Sciences
Protein Domains
Schizophrenia
Solutions
Tumbling
DUF1220
Autism
Protein domains
Schizophrenia
Gene duplication
Brain evolution
Backbone chemical shift assignment
Olduvai
ISSN:1874-2718, 1874-270X, 1874-270X
Online Access:Get full text
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Summary:Olduvai protein domains, encoded primarily by NBPF genes, have been linked to both human brain evolution and cognitive diseases such as autism and schizophrenia. There are six primary domains that comprise the Olduvai family: three conserved domains (CON1-3) and three human lineage-specific domains (HLS1-3), which typically occur as a triplet (HLS1, HLS2 and HLS3). Herein, we present the solution NMR assignment of the backbone chemical shifts of the separate HLS1, 2 and 3 domains of NBPF15. Our data suggest that there is no change in the structure of the separate domains when compared to the full-length triplet (HLS1–HLS2–HLS3). We also demonstrate that there is no direct interaction between the three domains.
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ISSN:1874-2718
1874-270X
1874-270X
DOI:10.1007/s12104-019-09902-0