Ultra-high-frequency radio-frequency acoustic molecular imaging with saline nanodroplets in living subjects
Molecular imaging is a crucial technique in clinical diagnostics but it relies on radioactive tracers or strong magnetic fields that are unsuitable for many patients, particularly infants and pregnant women. Ultra-high-frequency radio-frequency acoustic (UHF-RF-acoustic) imaging using non-ionizing R...
Gespeichert in:
| Veröffentlicht in: | Nature nanotechnology Jg. 16; H. 6; S. 717 - 724 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Journal Article |
| Sprache: | Englisch |
| Veröffentlicht: |
England
Nature Publishing Group
01.06.2021
|
| Schlagworte: | |
| ISSN: | 1748-3387, 1748-3395, 1748-3395 |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| Abstract | Molecular imaging is a crucial technique in clinical diagnostics but it relies on radioactive tracers or strong magnetic fields that are unsuitable for many patients, particularly infants and pregnant women. Ultra-high-frequency radio-frequency acoustic (UHF-RF-acoustic) imaging using non-ionizing RF pulses allows deep-tissue imaging with sub-millimetre spatial resolution. However, lack of biocompatible and targetable contrast agents has prevented the successful in vivo application of UHF-RF-acoustic imaging. Here we report our development of targetable nanodroplets for UHF-RF-acoustic molecular imaging of cancers. We synthesize all-liquid nanodroplets containing hypertonic saline that are stable for at least 2 weeks and can produce high-intensity UHF-RF-acoustic signals. Compared with concentration-matched iron oxide nanoparticles, our nanodroplets produce at least 1,600 times higher UHF-RF-acoustic signals at the same imaging depth. We demonstrate in vivo imaging using the targeted nanodroplets in a prostate cancer xenograft mouse model expressing gastrin release protein receptor (GRPR), and show that targeting specificity is increased by more than 2-fold compared with untargeted nanodroplets or prostate cancer cells not expressing this receptor. |
|---|---|
| AbstractList | Molecular imaging is a crucial technique in clinical diagnostics but it relies on radioactive tracers or strong magnetic fields that are unsuitable for many patients, particularly infants and pregnant women. Ultra-high-frequency radio-frequency acoustic (UHF-RF-acoustic) imaging using non-ionizing RF pulses allows deep-tissue imaging with sub-millimetre spatial resolution. However, lack of biocompatible and targetable contrast agents has prevented the successful in vivo application of UHF-RF-acoustic imaging. Here we report our development of targetable nanodroplets for UHF-RF-acoustic molecular imaging of cancers. We synthesize all-liquid nanodroplets containing hypertonic saline that are stable for at least 2 weeks and can produce high-intensity UHF-RF-acoustic signals. Compared with concentration-matched iron oxide nanoparticles, our nanodroplets produce at least 1,600 times higher UHF-RF-acoustic signals at the same imaging depth. We demonstrate in vivo imaging using the targeted nanodroplets in a prostate cancer xenograft mouse model expressing gastrin release protein receptor (GRPR), and show that targeting specificity is increased by more than 2-fold compared with untargeted nanodroplets or prostate cancer cells not expressing this receptor.Ultra-high-frequency radio-frequency acoustic molecular imaging is a safe molecular imaging diagnostic option because it does not require radioactive probes or high magnetic fields, but lack of biocompatible targeted contrast agents has so far limited its in vivo application. In this paper the authors present perfluorocarbon nanodroplets containing hypertonic saline solution for targeted molecular imaging of prostate cancer in animal models. Molecular imaging is a crucial technique in clinical diagnostics but it relies on radioactive tracers or strong magnetic fields that are unsuitable for many patients, particularly infants and pregnant women. Ultra-high-frequency radio-frequency acoustic (UHF-RF-acoustic) imaging using non-ionizing RF pulses allows deep-tissue imaging with sub-millimetre spatial resolution. However, lack of biocompatible and targetable contrast agents has prevented the successful in vivo application of UHF-RF-acoustic imaging. Here we report our development of targetable nanodroplets for UHF-RF-acoustic molecular imaging of cancers. We synthesize all-liquid nanodroplets containing hypertonic saline that are stable for at least 2 weeks and can produce high-intensity UHF-RF-acoustic signals. Compared with concentration-matched iron oxide nanoparticles, our nanodroplets produce at least 1,600 times higher UHF-RF-acoustic signals at the same imaging depth. We demonstrate in vivo imaging using the targeted nanodroplets in a prostate cancer xenograft mouse model expressing gastrin release protein receptor (GRPR), and show that targeting specificity is increased by more than 2-fold compared with untargeted nanodroplets or prostate cancer cells not expressing this receptor. Molecular imaging is a crucial technique in clinical diagnostics but it relies on radioactive tracers or strong magnetic fields that are unsuitable for many patients, particularly infants and pregnant women. Ultra-high-frequency radio-frequency acoustic (UHF-RF-acoustic) imaging using non-ionizing RF pulses allows deep-tissue imaging with sub-millimetre spatial resolution. However, lack of biocompatible and targetable contrast agents has prevented the successful in vivo application of UHF-RF-acoustic imaging. Here we report our development of targetable nanodroplets for UHF-RF-acoustic molecular imaging of cancers. We synthesize all-liquid nanodroplets containing hypertonic saline that are stable for at least 2 weeks and can produce high-intensity UHF-RF-acoustic signals. Compared with concentration-matched iron oxide nanoparticles, our nanodroplets produce at least 1,600 times higher UHF-RF-acoustic signals at the same imaging depth. We demonstrate in vivo imaging using the targeted nanodroplets in a prostate cancer xenograft mouse model expressing gastrin release protein receptor (GRPR), and show that targeting specificity is increased by more than 2-fold compared with untargeted nanodroplets or prostate cancer cells not expressing this receptor.Molecular imaging is a crucial technique in clinical diagnostics but it relies on radioactive tracers or strong magnetic fields that are unsuitable for many patients, particularly infants and pregnant women. Ultra-high-frequency radio-frequency acoustic (UHF-RF-acoustic) imaging using non-ionizing RF pulses allows deep-tissue imaging with sub-millimetre spatial resolution. However, lack of biocompatible and targetable contrast agents has prevented the successful in vivo application of UHF-RF-acoustic imaging. Here we report our development of targetable nanodroplets for UHF-RF-acoustic molecular imaging of cancers. We synthesize all-liquid nanodroplets containing hypertonic saline that are stable for at least 2 weeks and can produce high-intensity UHF-RF-acoustic signals. Compared with concentration-matched iron oxide nanoparticles, our nanodroplets produce at least 1,600 times higher UHF-RF-acoustic signals at the same imaging depth. We demonstrate in vivo imaging using the targeted nanodroplets in a prostate cancer xenograft mouse model expressing gastrin release protein receptor (GRPR), and show that targeting specificity is increased by more than 2-fold compared with untargeted nanodroplets or prostate cancer cells not expressing this receptor. |
| Author | Chen, Yun-Sheng Chen, Dong-Hua Dionne, Jennifer Zhao, Yang Stoyanova, Tanya Beinat, Corinne Hsu, En-Chi Achterberg, Friso Wang, Hanwei Zlitni, Aimen Gambhir, Sanjiv Sam |
| Author_xml | – sequence: 1 givenname: Yun-Sheng orcidid: 0000-0001-8823-970X surname: Chen fullname: Chen, Yun-Sheng email: yunsheng@illinois.edu, yunsheng@illinois.edu organization: Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA. yunsheng@illinois.edu – sequence: 2 givenname: Yang orcidid: 0000-0002-0154-3483 surname: Zhao fullname: Zhao, Yang organization: Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA – sequence: 3 givenname: Corinne orcidid: 0000-0003-1718-3272 surname: Beinat fullname: Beinat, Corinne organization: Department of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA, USA – sequence: 4 givenname: Aimen orcidid: 0000-0002-4790-1573 surname: Zlitni fullname: Zlitni, Aimen organization: Department of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA, USA – sequence: 5 givenname: En-Chi orcidid: 0000-0002-5671-6169 surname: Hsu fullname: Hsu, En-Chi organization: Department of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA, USA – sequence: 6 givenname: Dong-Hua orcidid: 0000-0002-5247-0106 surname: Chen fullname: Chen, Dong-Hua organization: Department of Structural Biology, Stanford University, Stanford, CA, USA – sequence: 7 givenname: Friso orcidid: 0000-0001-7007-859X surname: Achterberg fullname: Achterberg, Friso organization: Department of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA, USA – sequence: 8 givenname: Hanwei orcidid: 0000-0003-3762-3650 surname: Wang fullname: Wang, Hanwei organization: Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA – sequence: 9 givenname: Tanya orcidid: 0000-0003-0119-9747 surname: Stoyanova fullname: Stoyanova, Tanya organization: Canary Center at Stanford for Cancer Early Detection, Stanford University, Stanford, CA, USA – sequence: 10 givenname: Jennifer orcidid: 0000-0001-5287-4357 surname: Dionne fullname: Dionne, Jennifer organization: Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA – sequence: 11 givenname: Sanjiv Sam orcidid: 0000-0002-2711-7554 surname: Gambhir fullname: Gambhir, Sanjiv Sam organization: Department of Bioengineering, Stanford University, Stanford, CA, USA |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/33782588$$D View this record in MEDLINE/PubMed |
| BookMark | eNpdkE1LAzEQhoNU7If-AQ8S8OIlms9N9ijFLyh4seclm822qWlSk12l_94Vq4hzmXnhYZhnpmAUYrAAnBN8TTBTN5kTUQiEKUEYq6JE4ghMiOQKMVaK0e-s5BhMc95gLGhJ-QkYMyYVFUpNwOvSd0mjtVutUZvsW2-D2cOkGxf_ZG1inztn4DZ6a3qvE3RbvXJhBT9ct4ZZexcsDDrEJsWdt12GLkDv3r-Q3Ncba7p8Co5b7bM9O_QZWN7fvcwf0eL54Wl-u0CGS94hwhgTpqwZpVIZ3mJRKtkKxkuqpSSWSytMowvTCEkLy6Q1uC4JNpxqVjLMZuDqe-8uxUEgd9XWZWO918EOHhUVWBJeKF4M6OU_dBP7FIbrBorjoSQXA3VxoPp6a5tqlwb7tK9-3sg-AST7dyA |
| CitedBy_id | crossref_primary_10_1103_42ym_l7rc crossref_primary_10_1109_TMI_2024_3447125 crossref_primary_10_1007_s40242_021_1196_1 crossref_primary_10_1021_acs_langmuir_5c02011 crossref_primary_10_3389_fbioe_2023_1323316 crossref_primary_10_1016_j_rechem_2025_102690 crossref_primary_10_1038_s41467_023_41172_2 crossref_primary_10_1002_smtd_202401679 crossref_primary_10_1007_s00259_022_05726_8 crossref_primary_10_1038_s41551_022_00978_z crossref_primary_10_1002_adfm_202308437 crossref_primary_10_1002_smll_202406860 crossref_primary_10_1016_j_eng_2024_01_030 crossref_primary_10_1109_TMI_2023_3250647 crossref_primary_10_1038_s41566_024_01387_1 crossref_primary_10_1002_lpor_202400781 crossref_primary_10_1016_j_addr_2025_115551 crossref_primary_10_3390_pharmaceutics14112463 crossref_primary_10_1063_5_0216061 |
| ContentType | Journal Article |
| Copyright | The Author(s), under exclusive licence to Springer Nature Limited 2021. |
| Copyright_xml | – notice: The Author(s), under exclusive licence to Springer Nature Limited 2021. |
| DBID | CGR CUY CVF ECM EIF NPM 3V. 7QO 7U5 7X7 7XB 88E 8FD 8FE 8FG 8FH 8FI 8FJ 8FK ABJCF ABUWG AEUYN AFKRA ARAPS AZQEC BBNVY BENPR BGLVJ BHPHI CCPQU D1I DWQXO F28 FR3 FYUFA GHDGH GNUQQ HCIFZ K9. KB. L6V L7M LK8 M0S M1P M7P M7S P5Z P62 P64 PDBOC PHGZM PHGZT PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI PTHSS 7X8 |
| DOI | 10.1038/s41565-021-00869-5 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed ProQuest Central (Corporate) Biotechnology Research Abstracts Solid State and Superconductivity Abstracts Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection ProQuest Hospital Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Materials Science & Engineering ProQuest Central (Alumni) ProQuest One Sustainability ProQuest Central UK/Ireland Advanced Technologies & Computer Science Collection ProQuest Central Essentials Biological Science Collection ProQuest Central Technology Collection Natural Science Collection ProQuest One ProQuest Materials Science Collection ProQuest Central ANTE: Abstracts in New Technology & Engineering Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Materials Science Database ProQuest Engineering Collection Advanced Technologies Database with Aerospace Biological Sciences ProQuest Health & Medical Collection Medical Database Biological Science Database Engineering Database ProQuest advanced technologies & aerospace journals ProQuest Advanced Technologies & Aerospace Collection Biotechnology and BioEngineering Abstracts Materials Science Collection (ProQuest) ProQuest Central Premium ProQuest One Academic (New) ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic (retired) ProQuest One Academic UKI Edition Engineering Collection MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) ProQuest Central Student ProQuest Advanced Technologies & Aerospace Collection ProQuest Central Essentials SciTech Premium Collection ProQuest One Applied & Life Sciences ProQuest One Sustainability Health Research Premium Collection Natural Science Collection Health & Medical Research Collection Biological Science Collection ProQuest Central (New) ProQuest Medical Library (Alumni) Engineering Collection ANTE: Abstracts in New Technology & Engineering Advanced Technologies & Aerospace Collection Engineering Database ProQuest Biological Science Collection ProQuest One Academic Eastern Edition ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) Biological Science Database ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts ProQuest Health & Medical Complete ProQuest One Academic UKI Edition Solid State and Superconductivity Abstracts Engineering Research Database ProQuest One Academic ProQuest One Academic (New) Technology Collection Technology Research Database ProQuest One Academic Middle East (New) Materials Science Collection ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Central ProQuest Health & Medical Research Collection ProQuest Engineering Collection Biotechnology Research Abstracts Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Materials Science Database Advanced Technologies Database with Aerospace ProQuest Materials Science Collection ProQuest SciTech Collection Advanced Technologies & Aerospace Database ProQuest Medical Library Materials Science & Engineering Collection ProQuest Central (Alumni) MEDLINE - Academic |
| DatabaseTitleList | ProQuest Central Student MEDLINE MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: BENPR name: AUTh Library subscriptions: ProQuest Central url: https://www.proquest.com/central sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Engineering |
| EISSN | 1748-3395 |
| EndPage | 724 |
| ExternalDocumentID | 33782588 |
| Genre | Research Support, Non-U.S. Gov't Journal Article Research Support, N.I.H., Extramural |
| GrantInformation_xml | – fundername: NIGMS NIH HHS grantid: R21 GM139022 |
| GroupedDBID | --- -~X 0R~ 123 29M 39C 4.4 53G 5BI 5M7 5S5 6OB 70F 7X7 88E 8FE 8FG 8FH 8FI 8FJ 8R4 8R5 AARCD AAYZH ABAWZ ABDBF ABJCF ABJNI ABLJU ABUWG ACBWK ACGFS ACIWK ACPRK ACUHS ADBBV AENEX AEUYN AFANA AFBBN AFKRA AFLOW AFRAH AFSHS AFWHJ AGAYW AHBCP AHMBA AHOSX AHSBF AIBTJ ALFFA ALIPV ALMA_UNASSIGNED_HOLDINGS ALPWD ARAPS ARMCB ASPBG AVWKF AXYYD AZFZN BBNVY BENPR BGLVJ BHPHI BKKNO BPHCQ BVXVI CCPQU CGR CS3 CUY CVF D1I DB5 DU5 EBS ECM EE. EIF EJD EMOBN ESX EXGXG F5P FEDTE FQGFK FSGXE FYUFA HCIFZ HMCUK HVGLF HZ~ I-F KB. L6V LK8 M1P M7P M7S MM. NFIDA NNMJJ NPM O9- ODYON P2P P62 PDBOC PHGZT PQQKQ PROAC PSQYO PTHSS Q2X RNS RNT RNTTT SHXYY SIXXV SNYQT SOJ SV3 TAOOD TBHMF TDRGL TSG TUS UKHRP ~8M 3V. 7QO 7U5 7XB 8FD 8FK ABFSG ACSTC AEZWR AFHIU AGSTI AHWEU AIXLP ATHPR AZQEC DWQXO F28 FR3 GNUQQ K9. L7M P64 PHGZM PJZUB PKEHL PPXIY PQEST PQGLB PQUKI 7X8 PUEGO |
| ID | FETCH-LOGICAL-c474t-13335c9b32278c4f05987f53492a771e47e5cda6cd5726e37ec0b910c42a39303 |
| IEDL.DBID | M7S |
| ISICitedReferencesCount | 30 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000634625300001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 1748-3387 1748-3395 |
| IngestDate | Wed Oct 01 13:57:18 EDT 2025 Sat Nov 29 15:02:34 EST 2025 Thu Apr 03 07:00:57 EDT 2025 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 6 |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c474t-13335c9b32278c4f05987f53492a771e47e5cda6cd5726e37ec0b910c42a39303 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 |
| ORCID | 0000-0002-2711-7554 0000-0003-0119-9747 0000-0002-5671-6169 0000-0001-7007-859X 0000-0003-3762-3650 0000-0002-0154-3483 0000-0001-8823-970X 0000-0003-1718-3272 0000-0001-5287-4357 0000-0002-5247-0106 0000-0002-4790-1573 |
| OpenAccessLink | https://www.ncbi.nlm.nih.gov/pmc/articles/8454903 |
| PMID | 33782588 |
| PQID | 2540000745 |
| PQPubID | 546299 |
| PageCount | 8 |
| ParticipantIDs | proquest_miscellaneous_2507146846 proquest_journals_2540000745 pubmed_primary_33782588 |
| PublicationCentury | 2000 |
| PublicationDate | 2021-06-01 |
| PublicationDateYYYYMMDD | 2021-06-01 |
| PublicationDate_xml | – month: 06 year: 2021 text: 2021-06-01 day: 01 |
| PublicationDecade | 2020 |
| PublicationPlace | England |
| PublicationPlace_xml | – name: England – name: London |
| PublicationTitle | Nature nanotechnology |
| PublicationTitleAlternate | Nat Nanotechnol |
| PublicationYear | 2021 |
| Publisher | Nature Publishing Group |
| Publisher_xml | – name: Nature Publishing Group |
| References | 34903031 - J Urol. 2022 Mar;207(3):727-729. doi: 10.1097/JU.0000000000002363 |
| References_xml | – reference: 34903031 - J Urol. 2022 Mar;207(3):727-729. doi: 10.1097/JU.0000000000002363 |
| SSID | ssj0052924 |
| Score | 2.5039337 |
| Snippet | Molecular imaging is a crucial technique in clinical diagnostics but it relies on radioactive tracers or strong magnetic fields that are unsuitable for many... |
| SourceID | proquest pubmed |
| SourceType | Aggregation Database Index Database |
| StartPage | 717 |
| SubjectTerms | Acoustic imaging Acoustics Animal models Animals Biocompatibility Cell Line, Tumor Chemical synthesis Contrast agents Contrast media Contrast Media - chemistry Drug Stability Gastrin Humans Hydrocarbons, Fluorinated - chemistry Iron oxides Magnetic fields Male Medical imaging Mice Mice, Inbred NOD Molecular Imaging - instrumentation Molecular Imaging - methods Nanoparticles Nanostructures - chemistry Perfluorocarbons Phantoms, Imaging Prostate cancer Prostatic Neoplasms - diagnostic imaging Prostatic Neoplasms - metabolism Radio frequency Radio Waves Radioactive tracers Receptors Receptors, Bombesin - genetics Receptors, Bombesin - immunology Receptors, Bombesin - metabolism Saline Solution, Hypertonic - chemistry Saline solutions Spatial discrimination Spatial resolution Ultrahigh frequencies Xenograft Model Antitumor Assays Xenografts Xenotransplantation |
| Title | Ultra-high-frequency radio-frequency acoustic molecular imaging with saline nanodroplets in living subjects |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/33782588 https://www.proquest.com/docview/2540000745 https://www.proquest.com/docview/2507146846 |
| Volume | 16 |
| WOSCitedRecordID | wos000634625300001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3fT9swED7RwsN4AMZ-UGCVkfbqNYnjOHlCFLWaNK2KxpCqvVSO40gRkEJSkPjvuXNS4GW88HKSkyhKnPPlu_PdfQDfM2NEkvsx13mieRgZn2ubeLyIpB-hw5H5XuHIJtRsFs_nSdoF3JourXJtE52hzpeGYuQjdGTctlsoT2_vOLFG0e5qR6HRg03qkhC41L2LtSWWQdKS2qow5uiKqa5oxhPxqCHHhWqT0ZlGVJ9w-X-I6X410933PuQe7HQgk521WvERNmy1D9uvWg9-gqvL61WtOXUr5kXd5lM_slrn5fLVGM2lY_tiN2sWXVbeOF4jRgFc1mhCqazS1TKvKRV91bCyYtclhSlYc59RlKf5DJfTyd_zn7wjXuAmVCHR0wshTZIJqpM1YYEQLFaFpEaGWinfhspKk-vI5FIFkRXKGi9D3GHCQIsEf4pfoF8tK3sArAhMXshIWIMLX6Iv7hdeJhEGBXhIKjOA4_VMLrrV0yxepnEAJ8-nUe9pM0NXFl8dr6HSqwjh0wC-tl9rcds26FgIgbhHxvHh2zc_gg-BUwCKqRxDf1Xf22-wZR5WZVMPoafmysl4CJvjySz9g6Nf4x9DUrLUyQuUs_Q3ylT-ewJ2ddl2 |
| linkProvider | ProQuest |
| linkToHtml | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1NT9wwEB0BRQIO0AJtlwJ1JThaJLEdx4cKoVIEgq56AGlvwXEcKSpkIVmo-FP9jZ1JNsClvXHgmA9ZcjwZvzeemQewkzknTB4m3ObGchm7kFtvAl7EKoyRcGRhULRiE3o4TEYj83MG_vS1MJRW2fvE1lHnY0cx8j0kMu2xm1T7N7ecVKPodLWX0OjM4tQ__EbK1nw9OcT13Y2io-_n3475VFWAO6klaa8LoZzJBBWBOlkgvkh0oahLn9U69FJ75XIbu1zpKPZCexdkuKk6GVlh0OPjuLPwBv24phQyPXokeCoynYiulglH6qenRTqBSPYaIkpUC43kHVmE4erfkLbd2o5WXttHeQvLUxDNDjqrfwczvlqFpWetFdfg18XVpLacujHzou7yxR9YbfNy_Owat4NWzYxd9yrBrLxudZsYBahZYwmFs8pW47ymVPtJw8qKXZUUhmHNXUZRrGYdLl5ktu9hrhpX_iOwInJ5oWLhHTo2JWMTFkGmEOZFeEtpN4DNfuXSqXdo0qdlG8CXx8f4X9Nhja08Th3fodKyGOHhAD501pHedA1IUiEQ16kk2fj_4J9h4fj8x1l6djI8_QSLUWt8FD_ahLlJfee3YN7dT8qm3m7NmMHlS5vIX8gQLA4 |
| linkToPdf | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1LT9wwEB7BUqFygLa8ltLWSO3R2iSO4-SAqqp0VUS12gNIiEvqOLYUAVmaLFT71_rrmMmjcGlvHDgmsSw5Hs_MN56ZD-BjZoxIcj_mOk80DyPjc20Tj7tI-hECjsz3XEM2oSaT-Pw8mS7Bn74WhtIqe53YKOp8ZihGPkIg01y7hXLkurSI6dH4880vTgxSdNPa02m0InJiF78RvtWHx0e415-CYPzt9Ot33jEMcBOqkHjYhZAmyQQVhJrQoa8RKyepY59WyrehstLkOjK5VEFkhbLGy9DAmjDQIkHtj_Muw4pCjEnAbyoveisgg6Ql1FVhzBEGqq5gxxPxqCbQRHXRCOQRUSRc_tu9bczceOM5_6BXsN451-xLexpew5It38Dao5aLm3B5djWvNKcuzdxVbR75glU6L2aPntFMNCxn7LpnD2bFdcPnxChwzWpN3jkrdTnLK0rBn9esKNlVQeEZVt9mFN2qt-DsSVa7DYNyVtpdYC4wuZORsAYVnkT58J2XSXT_AnwllRnCfr-Laac16vRhC4dw8Pcznne6xNGlxaXjGCo5i9BtHMJOKynpTduYJBUC_T0Zx3v_n_wDrKJkpD-OJydv4WXQyCGFlfZhMK9u7Tt4Ye7mRV29bySawc-nlpB7bFM1AQ |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Ultra-high-frequency+radio-frequency+acoustic+molecular+imaging+with+saline+nanodroplets+in+living+subjects&rft.jtitle=Nature+nanotechnology&rft.au=Yun-Sheng%2C+Chen&rft.au=Zhao%2C+Yang&rft.au=Beinat+Corinne&rft.au=Zlitni+Aimen&rft.date=2021-06-01&rft.pub=Nature+Publishing+Group&rft.issn=1748-3387&rft.eissn=1748-3395&rft.volume=16&rft.issue=6&rft.spage=717&rft.epage=724&rft_id=info:doi/10.1038%2Fs41565-021-00869-5&rft.externalDBID=HAS_PDF_LINK |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1748-3387&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1748-3387&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1748-3387&client=summon |