Profiling of lipid species by normal-phase liquid chromatography, nanoelectrospray ionization, and ion trap–orbitrap mass spectrometry

Detailed analysis of lipid species can be challenging due to their structural diversity and wide concentration range in cells, tissues, and biofluids. To address these analytical challenges, we devised a reproducible, sensitive, and integrated lipidomics workflow based on normal-phase liquid chromat...

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Bibliographic Details
Published in:	Analytical biochemistry Vol. 443; no. 1; pp. 88 - 96
Main Authors:	Sokol, Elena, Almeida, Reinaldo, Hannibal-Bach, Hans Kristian, Kotowska, Dorota, Vogt, Johannes, Baumgart, Jan, Kristiansen, Karsten, Nitsch, Robert, Knudsen, Jens, Ejsing, Christer S.
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01.12.2013
Subjects:	3T3-L1 Cells - chemistry adipocytes Animals ceramides Ceramides - classification Ceramides - isolation & purification cerebellum Cerebellum - chemistry Chromatography, Liquid fatty acids Hydrophobic and Hydrophilic Interactions ionization Lipidomics liquid chromatography Mice Mice, Inbred C57BL Nanoelectrospray ionization Normal-phase LC Orbitrap mass spectrometry spectrometers Spectrometry, Mass, Electrospray Ionization surveys Tandem Mass Spectrometry tissues triacylglycerols Triglycerides - classification Triglycerides - isolation & purification Lipidomics Normal-phase LC Orbitrap mass spectrometry Nanoelectrospray ionization
ISSN:	0003-2697, 1096-0309, 1096-0309
Online Access:	Get full text
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