Profiling of lipid species by normal-phase liquid chromatography, nanoelectrospray ionization, and ion trap–orbitrap mass spectrometry

Detailed analysis of lipid species can be challenging due to their structural diversity and wide concentration range in cells, tissues, and biofluids. To address these analytical challenges, we devised a reproducible, sensitive, and integrated lipidomics workflow based on normal-phase liquid chromat...

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Veröffentlicht in:	Analytical biochemistry Jg. 443; H. 1; S. 88 - 96
Hauptverfasser:	Sokol, Elena, Almeida, Reinaldo, Hannibal-Bach, Hans Kristian, Kotowska, Dorota, Vogt, Johannes, Baumgart, Jan, Kristiansen, Karsten, Nitsch, Robert, Knudsen, Jens, Ejsing, Christer S.
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States Elsevier Inc 01.12.2013
Schlagworte:	3T3-L1 Cells - chemistry adipocytes Animals ceramides Ceramides - classification Ceramides - isolation & purification cerebellum Cerebellum - chemistry Chromatography, Liquid fatty acids Hydrophobic and Hydrophilic Interactions ionization Lipidomics liquid chromatography Mice Mice, Inbred C57BL Nanoelectrospray ionization Normal-phase LC Orbitrap mass spectrometry spectrometers Spectrometry, Mass, Electrospray Ionization surveys Tandem Mass Spectrometry tissues triacylglycerols Triglycerides - classification Triglycerides - isolation & purification Lipidomics Normal-phase LC Orbitrap mass spectrometry Nanoelectrospray ionization
ISSN:	0003-2697, 1096-0309, 1096-0309
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Abstract	Detailed analysis of lipid species can be challenging due to their structural diversity and wide concentration range in cells, tissues, and biofluids. To address these analytical challenges, we devised a reproducible, sensitive, and integrated lipidomics workflow based on normal-phase liquid chromatography–Fourier transform mass spectrometry (LC–FTMS) and LC–ITMS2 (ion trap tandem mass spectrometry) for profiling and structural analysis of lipid species. The workflow uses a normal-phase LC system for efficient separation of apolar and polar lipid species combined with sensitive and specific analysis powered by a chip-based nanoelectrospray ion source and a hybrid ion trap–orbitrap mass spectrometer. The workflow was executed using a primary LC–FTMS survey routine for identification and profiling of lipid species based on high-mass accuracy and retention time followed by a targeted LC–ITMS2 routine for characterizing the fatty acid moieties of identified lipid species. We benchmarked the performance of the workflow by characterizing the chromatographic properties of the LC–MS system for general lipid analysis. In addition, we demonstrate the efficacy of the workflow by reporting a study of low-abundant triacylglycerol and ceramide species in mouse brain cerebellum and 3T3-L1 adipocytes, respectively. The workflow described here is generic and can be extended for detailed lipid analysis of sample matrices having a wide range of lipid compositions.
AbstractList	Detailed analysis of lipid species can be challenging due to their structural diversity and wide concentration range in cells, tissues, and biofluids. To address these analytical challenges, we devised a reproducible, sensitive, and integrated lipidomics workflow based on normal-phase liquid chromatography-Fourier transform mass spectrometry (LC-FTMS) and LC-ITMS(2) (ion trap tandem mass spectrometry) for profiling and structural analysis of lipid species. The workflow uses a normal-phase LC system for efficient separation of apolar and polar lipid species combined with sensitive and specific analysis powered by a chip-based nanoelectrospray ion source and a hybrid ion trap-orbitrap mass spectrometer. The workflow was executed using a primary LC-FTMS survey routine for identification and profiling of lipid species based on high-mass accuracy and retention time followed by a targeted LC-ITMS(2) routine for characterizing the fatty acid moieties of identified lipid species. We benchmarked the performance of the workflow by characterizing the chromatographic properties of the LC-MS system for general lipid analysis. In addition, we demonstrate the efficacy of the workflow by reporting a study of low-abundant triacylglycerol and ceramide species in mouse brain cerebellum and 3T3-L1 adipocytes, respectively. The workflow described here is generic and can be extended for detailed lipid analysis of sample matrices having a wide range of lipid compositions. Detailed analysis of lipid species can be challenging due to their structural diversity and wide concentration range in cells, tissues, and biofluids. To address these analytical challenges, we devised a reproducible, sensitive, and integrated lipidomics workflow based on normal-phase liquid chromatography–Fourier transform mass spectrometry (LC–FTMS) and LC–ITMS² (ion trap tandem mass spectrometry) for profiling and structural analysis of lipid species. The workflow uses a normal-phase LC system for efficient separation of apolar and polar lipid species combined with sensitive and specific analysis powered by a chip-based nanoelectrospray ion source and a hybrid ion trap–orbitrap mass spectrometer. The workflow was executed using a primary LC–FTMS survey routine for identification and profiling of lipid species based on high-mass accuracy and retention time followed by a targeted LC–ITMS² routine for characterizing the fatty acid moieties of identified lipid species. We benchmarked the performance of the workflow by characterizing the chromatographic properties of the LC–MS system for general lipid analysis. In addition, we demonstrate the efficacy of the workflow by reporting a study of low-abundant triacylglycerol and ceramide species in mouse brain cerebellum and 3T3-L1 adipocytes, respectively. The workflow described here is generic and can be extended for detailed lipid analysis of sample matrices having a wide range of lipid compositions. Detailed analysis of lipid species can be challenging due to their structural diversity and wide concentration range in cells, tissues, and biofluids. To address these analytical challenges, we devised a reproducible, sensitive, and integrated lipidomics workflow based on normal-phase liquid chromatography-Fourier transform mass spectrometry (LC-FTMS) and LC-ITMS(2) (ion trap tandem mass spectrometry) for profiling and structural analysis of lipid species. The workflow uses a normal-phase LC system for efficient separation of apolar and polar lipid species combined with sensitive and specific analysis powered by a chip-based nanoelectrospray ion source and a hybrid ion trap-orbitrap mass spectrometer. The workflow was executed using a primary LC-FTMS survey routine for identification and profiling of lipid species based on high-mass accuracy and retention time followed by a targeted LC-ITMS(2) routine for characterizing the fatty acid moieties of identified lipid species. We benchmarked the performance of the workflow by characterizing the chromatographic properties of the LC-MS system for general lipid analysis. In addition, we demonstrate the efficacy of the workflow by reporting a study of low-abundant triacylglycerol and ceramide species in mouse brain cerebellum and 3T3-L1 adipocytes, respectively. The workflow described here is generic and can be extended for detailed lipid analysis of sample matrices having a wide range of lipid compositions.Detailed analysis of lipid species can be challenging due to their structural diversity and wide concentration range in cells, tissues, and biofluids. To address these analytical challenges, we devised a reproducible, sensitive, and integrated lipidomics workflow based on normal-phase liquid chromatography-Fourier transform mass spectrometry (LC-FTMS) and LC-ITMS(2) (ion trap tandem mass spectrometry) for profiling and structural analysis of lipid species. The workflow uses a normal-phase LC system for efficient separation of apolar and polar lipid species combined with sensitive and specific analysis powered by a chip-based nanoelectrospray ion source and a hybrid ion trap-orbitrap mass spectrometer. The workflow was executed using a primary LC-FTMS survey routine for identification and profiling of lipid species based on high-mass accuracy and retention time followed by a targeted LC-ITMS(2) routine for characterizing the fatty acid moieties of identified lipid species. We benchmarked the performance of the workflow by characterizing the chromatographic properties of the LC-MS system for general lipid analysis. In addition, we demonstrate the efficacy of the workflow by reporting a study of low-abundant triacylglycerol and ceramide species in mouse brain cerebellum and 3T3-L1 adipocytes, respectively. The workflow described here is generic and can be extended for detailed lipid analysis of sample matrices having a wide range of lipid compositions. Detailed analysis of lipid species can be challenging due to their structural diversity and wide concentration range in cells, tissues, and biofluids. To address these analytical challenges, we devised a reproducible, sensitive, and integrated lipidomics workflow based on normal-phase liquid chromatography–Fourier transform mass spectrometry (LC–FTMS) and LC–ITMS2 (ion trap tandem mass spectrometry) for profiling and structural analysis of lipid species. The workflow uses a normal-phase LC system for efficient separation of apolar and polar lipid species combined with sensitive and specific analysis powered by a chip-based nanoelectrospray ion source and a hybrid ion trap–orbitrap mass spectrometer. The workflow was executed using a primary LC–FTMS survey routine for identification and profiling of lipid species based on high-mass accuracy and retention time followed by a targeted LC–ITMS2 routine for characterizing the fatty acid moieties of identified lipid species. We benchmarked the performance of the workflow by characterizing the chromatographic properties of the LC–MS system for general lipid analysis. In addition, we demonstrate the efficacy of the workflow by reporting a study of low-abundant triacylglycerol and ceramide species in mouse brain cerebellum and 3T3-L1 adipocytes, respectively. The workflow described here is generic and can be extended for detailed lipid analysis of sample matrices having a wide range of lipid compositions.
Author	Vogt, Johannes Baumgart, Jan Knudsen, Jens Ejsing, Christer S. Nitsch, Robert Almeida, Reinaldo Kristiansen, Karsten Sokol, Elena Hannibal-Bach, Hans Kristian Kotowska, Dorota
Author_xml	– sequence: 1 givenname: Elena surname: Sokol fullname: Sokol, Elena organization: Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230 Odense, Denmark – sequence: 2 givenname: Reinaldo surname: Almeida fullname: Almeida, Reinaldo organization: Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230 Odense, Denmark – sequence: 3 givenname: Hans Kristian surname: Hannibal-Bach fullname: Hannibal-Bach, Hans Kristian organization: Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230 Odense, Denmark – sequence: 4 givenname: Dorota surname: Kotowska fullname: Kotowska, Dorota organization: Department of Biology, University of Copenhagen, DK-2200 Copenhagen, Denmark – sequence: 5 givenname: Johannes surname: Vogt fullname: Vogt, Johannes organization: Institute for Microscopic Anatomy and Neurobiology, Johannes Gutenberg University of Mainz, D-55128 Mainz, Germany – sequence: 6 givenname: Jan surname: Baumgart fullname: Baumgart, Jan organization: Institute for Microscopic Anatomy and Neurobiology, Johannes Gutenberg University of Mainz, D-55128 Mainz, Germany – sequence: 7 givenname: Karsten surname: Kristiansen fullname: Kristiansen, Karsten organization: Department of Biology, University of Copenhagen, DK-2200 Copenhagen, Denmark – sequence: 8 givenname: Robert surname: Nitsch fullname: Nitsch, Robert organization: Institute for Microscopic Anatomy and Neurobiology, Johannes Gutenberg University of Mainz, D-55128 Mainz, Germany – sequence: 9 givenname: Jens surname: Knudsen fullname: Knudsen, Jens organization: Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230 Odense, Denmark – sequence: 10 givenname: Christer S. surname: Ejsing fullname: Ejsing, Christer S. email: cse@bmb.sdu.dk organization: Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230 Odense, Denmark
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Cites_doi	10.1016/S1044-0305(02)00358-6 10.1021/ac00017a023 10.1073/pnas.0811700106 10.1146/annurev-biochem-060409-092612 10.1038/nrm2335 10.1073/pnas.94.6.2339 10.1016/j.ab.2007.12.027 10.1021/ac060545x 10.2337/db06-0330 10.1016/j.jchromb.2009.02.037 10.1074/jbc.M702719200 10.1038/nrm2934 10.1016/j.ab.2011.03.009 10.1146/annurev.anchem.111808.073705 10.1016/S0021-9673(01)01137-2 10.1016/j.prostaglandins.2004.09.016 10.1073/pnas.1019267108 10.1194/jlr.D800001-JLR200 10.1006/abio.2001.5536 10.1016/j.jasms.2005.04.017 10.1021/ac051605m 10.1021/ac048489s 10.1021/ac062455y 10.1038/sj.emboj.7600798 10.1002/mas.20342 10.1038/nrd1776 10.1194/jlr.M500506-JLR200
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Keywords	Lipidomics Normal-phase LC Orbitrap mass spectrometry Nanoelectrospray ionization
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References	Harkewicz, Dennis (b0025) 2011; 80 Ejsing, Duchoslav, Sampaio, Simons, Bonner, Thiele, Ekroos, Shevchenko (b0055) 2006; 78 Benghezal, Roubaty, Veepuri, Knudsen, Conzelmann (b0120) 2007; 282 Brouwers (b0100) 1811; 2011 Wenk (b0035) 2005; 4 Duffin, Henion, Shieh (b0125) 1991; 63 Blanksby, Mitchell (b0030) 2010; 3 Schwudke, Hannich, Surendranath, Grimard, Moehring, Burton, Kurzchalia, Shevchenko (b0060) 2007; 79 Serhan (b0040) 2005; 77 Stahlman, Ejsing, Tarasov, Perman, Boren, Ekroos (b0070) 2009; 877 McLaren, Miller, Lassman, Castro-Perez, Hubbard, Roddy (b0105) 2011; 414 Ejsing, Sampaio, Surendranath, Duchoslav, Ekroos, Klemm, Simons, Shevchenko (b0065) 2009; 106 Sommer, Herscovitz, Welty, Costello (b0095) 2006; 47 Sullards, Liu, Chen, Merrill (b0045) 1811; 2011 Myers, Ivanova, Milne, Brown (b0075) 1811; 2011 Shevchenko, Simons (b0005) 2010; 11 McAnoy, Wu, Murphy (b0130) 2005; 16 Schwudke, Oegema, Burton, Entchev, Hannich, Ejsing, Kurzchalia, Shevchenko (b0135) 2006; 78 Ogiso, Suzuki, Taguchi (b0080) 2008; 375 Sampaio, Gerl, Klose, Ejsing, Beug, Simons, Shevchenko (b0110) 2011; 108 Hermansson, Uphoff, Kakela, Somerharju (b0090) 2005; 77 Han (b0140) 2002; 302 van Meer (b0010) 2005; 24 Han, Yang, Gross (b0020) 2012; 31 Brugger, Erben, Sandhoff, Wieland, Lehmann (b0050) 1997; 94 Deschamps, Chaminade, Ferrier, Baillet (b0115) 2001; 928 Samad, Hester, Yang, Hannun, Bielawski (b0150) 2006; 55 Hsu, Turk (b0145) 2002; 13 Haynes, Allegood, Sims, Wang, Sullards, Merrill (b0085) 2008; 49 Wymann, Schneiter (b0015) 2008; 9 Schwudke (10.1016/j.ab.2013.08.020_b0135) 2006; 78 Shevchenko (10.1016/j.ab.2013.08.020_b0005) 2010; 11 Harkewicz (10.1016/j.ab.2013.08.020_b0025) 2011; 80 Hermansson (10.1016/j.ab.2013.08.020_b0090) 2005; 77 Sommer (10.1016/j.ab.2013.08.020_b0095) 2006; 47 Blanksby (10.1016/j.ab.2013.08.020_b0030) 2010; 3 Myers (10.1016/j.ab.2013.08.020_b0075) 1811; 2011 Benghezal (10.1016/j.ab.2013.08.020_b0120) 2007; 282 Haynes (10.1016/j.ab.2013.08.020_b0085) 2008; 49 Duffin (10.1016/j.ab.2013.08.020_b0125) 1991; 63 Stahlman (10.1016/j.ab.2013.08.020_b0070) 2009; 877 Han (10.1016/j.ab.2013.08.020_b0140) 2002; 302 van Meer (10.1016/j.ab.2013.08.020_b0010) 2005; 24 Wymann (10.1016/j.ab.2013.08.020_b0015) 2008; 9 Han (10.1016/j.ab.2013.08.020_b0020) 2012; 31 Sampaio (10.1016/j.ab.2013.08.020_b0110) 2011; 108 McLaren (10.1016/j.ab.2013.08.020_b0105) 2011; 414 Deschamps (10.1016/j.ab.2013.08.020_b0115) 2001; 928 McAnoy (10.1016/j.ab.2013.08.020_b0130) 2005; 16 Serhan (10.1016/j.ab.2013.08.020_b0040) 2005; 77 Ejsing (10.1016/j.ab.2013.08.020_b0055) 2006; 78 Hsu (10.1016/j.ab.2013.08.020_b0145) 2002; 13 Schwudke (10.1016/j.ab.2013.08.020_b0060) 2007; 79 Ogiso (10.1016/j.ab.2013.08.020_b0080) 2008; 375 Brugger (10.1016/j.ab.2013.08.020_b0050) 1997; 94 Sullards (10.1016/j.ab.2013.08.020_b0045) 1811; 2011 Wenk (10.1016/j.ab.2013.08.020_b0035) 2005; 4 Ejsing (10.1016/j.ab.2013.08.020_b0065) 2009; 106 Brouwers (10.1016/j.ab.2013.08.020_b0100) 1811; 2011 Samad (10.1016/j.ab.2013.08.020_b0150) 2006; 55
References_xml	– volume: 11 start-page: 593 year: 2010 end-page: 598 ident: b0005 article-title: Lipidomics: coming to grips with lipid diversity publication-title: Nat. Rev. Mol. Cell. Biol. – volume: 78 start-page: 585 year: 2006 end-page: 595 ident: b0135 article-title: Lipid profiling by multiple precursor and neutral loss scanning driven by the data-dependent acquisition publication-title: Anal. Chem. – volume: 9 start-page: 162 year: 2008 end-page: 176 ident: b0015 article-title: Lipid signalling in disease publication-title: Nat. Rev. Mol. Cell. Biol. – volume: 49 start-page: 1113 year: 2008 end-page: 1125 ident: b0085 article-title: Quantitation of fatty acyl-coenzyme As in mammalian cells by liquid chromatography–electrospray ionization tandem mass spectrometry publication-title: J. Lipid Res. – volume: 2011 start-page: 763 year: 1811 end-page: 775 ident: b0100 article-title: Liquid chromatographic–mass spectrometric analysis of phospholipids: chromatography, ionization, and quantification publication-title: Biochim. Biophys. Acta – volume: 13 start-page: 558 year: 2002 end-page: 570 ident: b0145 article-title: Characterization of ceramides by low energy collisional-activated dissociation tandem mass spectrometry with negative-ion electrospray ionization publication-title: J. Am. Soc. Mass Spectrom. – volume: 375 start-page: 124 year: 2008 end-page: 131 ident: b0080 article-title: Development of a reverse-phase liquid chromatography electrospray ionization mass spectrometry method for lipidomics, improving detection of phosphatidic acid and phosphatidylserine publication-title: Anal. Biochem. – volume: 2011 start-page: 838 year: 1811 end-page: 853 ident: b0045 article-title: Analysis of mammalian sphingolipids by liquid chromatography tandem mass spectrometry (LC–MS/MS) and tissue imaging mass spectrometry (TIMS) publication-title: Biochim. Biophys. Acta – volume: 79 start-page: 4083 year: 2007 end-page: 4093 ident: b0060 article-title: Top-down lipidomic screens by multivariate analysis of high-resolution survey mass spectra publication-title: Anal. Chem. – volume: 877 start-page: 2664 year: 2009 end-page: 2672 ident: b0070 article-title: High-throughput shotgun lipidomics by quadrupole time-of-flight mass spectrometry publication-title: J. Chromatogr. B – volume: 47 start-page: 804 year: 2006 end-page: 814 ident: b0095 article-title: LC–MS-based method for the qualitative and quantitative analysis of complex lipid mixtures publication-title: J. Lipid Res. – volume: 928 start-page: 127 year: 2001 end-page: 137 ident: b0115 article-title: Assessment of the retention properties of poly(vinyl alcohol) stationary phase for lipid class profiling in liquid chromatography publication-title: J. Chromatogr. A – volume: 3 start-page: 433 year: 2010 end-page: 465 ident: b0030 article-title: Advances in mass spectrometry for lipidomics publication-title: Annu. Rev. Anal. Chem. – volume: 24 start-page: 3159 year: 2005 end-page: 3165 ident: b0010 article-title: Cellular lipidomics publication-title: EMBO J. – volume: 94 start-page: 2339 year: 1997 end-page: 2344 ident: b0050 article-title: Quantitative analysis of biological membrane lipids at the low picomole level by nano-electrospray ionization tandem mass spectrometry publication-title: Proc. Natl. Acad. Sci. USA – volume: 108 start-page: 1903 year: 2011 end-page: 1907 ident: b0110 article-title: Membrane lipidome of an epithelial cell line publication-title: Proc. Natl. Acad. Sci. USA – volume: 302 start-page: 199 year: 2002 end-page: 212 ident: b0140 article-title: Characterization and direct quantitation of ceramide molecular species from lipid extracts of biological samples by electrospray ionization tandem mass spectrometry publication-title: Anal. Biochem. – volume: 4 start-page: 594 year: 2005 end-page: 610 ident: b0035 article-title: The emerging field of lipidomics publication-title: Nat. Rev. Drug Discov. – volume: 63 start-page: 1781 year: 1991 end-page: 1788 ident: b0125 article-title: Electrospray and tandem mass spectrometric characterization of acylglycerol mixtures that are dissolved in nonpolar solvents publication-title: Anal. Chem. – volume: 77 start-page: 4 year: 2005 end-page: 14 ident: b0040 article-title: Mediator lipidomics publication-title: Prostaglandins Other Lipid Mediat. – volume: 80 start-page: 301 year: 2011 end-page: 325 ident: b0025 article-title: Applications of mass spectrometry to lipids and membranes publication-title: Annu. Rev. Biochem. – volume: 106 start-page: 2136 year: 2009 end-page: 2141 ident: b0065 article-title: Global analysis of the yeast lipidome by quantitative shotgun mass spectrometry publication-title: Proc. Natl. Acad. Sci. USA – volume: 414 start-page: 266 year: 2011 end-page: 272 ident: b0105 article-title: An ultraperformance liquid chromatography method for the normal-phase separation of lipids publication-title: Anal. Biochem. – volume: 31 start-page: 134 year: 2012 end-page: 178 ident: b0020 article-title: Multi-dimensional mass spectrometry-based shotgun lipidomics and novel strategies for lipidomic analyses publication-title: Mass Spectrom. Rev. – volume: 55 start-page: 2579 year: 2006 end-page: 2587 ident: b0150 article-title: Altered adipose and plasma sphingolipid metabolism in obesity: a potential mechanism for cardiovascular and metabolic risk publication-title: Diabetes – volume: 2011 start-page: 748 year: 1811 end-page: 757 ident: b0075 article-title: Quantitative analysis of glycerophospholipids by LC–MS: acquisition, data handling, and interpretation publication-title: Biochim. Biophys. Acta – volume: 78 start-page: 6202 year: 2006 end-page: 6214 ident: b0055 article-title: Automated identification and quantification of glycerophospholipid molecular species by multiple precursor ion scanning publication-title: Anal. Chem. – volume: 77 start-page: 2166 year: 2005 end-page: 2175 ident: b0090 article-title: Automated quantitative analysis of complex lipidomes by liquid chromatography/mass spectrometry publication-title: Anal. Chem. – volume: 16 start-page: 1498 year: 2005 end-page: 1509 ident: b0130 article-title: Direct qualitative analysis of triacylglycerols by electrospray mass spectrometry using a linear ion trap publication-title: J. Am. Soc. Mass Spectrom. – volume: 282 start-page: 30845 year: 2007 end-page: 30855 ident: b0120 article-title: SLC1 and SLC4 encode partially redundant acyl-coenzyme A 1-acylglycerol-3-phosphate publication-title: J. Biol. Chem. – volume: 13 start-page: 558 year: 2002 ident: 10.1016/j.ab.2013.08.020_b0145 article-title: Characterization of ceramides by low energy collisional-activated dissociation tandem mass spectrometry with negative-ion electrospray ionization publication-title: J. Am. Soc. Mass Spectrom. doi: 10.1016/S1044-0305(02)00358-6 – volume: 63 start-page: 1781 year: 1991 ident: 10.1016/j.ab.2013.08.020_b0125 article-title: Electrospray and tandem mass spectrometric characterization of acylglycerol mixtures that are dissolved in nonpolar solvents publication-title: Anal. Chem. doi: 10.1021/ac00017a023 – volume: 106 start-page: 2136 year: 2009 ident: 10.1016/j.ab.2013.08.020_b0065 article-title: Global analysis of the yeast lipidome by quantitative shotgun mass spectrometry publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.0811700106 – volume: 80 start-page: 301 year: 2011 ident: 10.1016/j.ab.2013.08.020_b0025 article-title: Applications of mass spectrometry to lipids and membranes publication-title: Annu. Rev. Biochem. doi: 10.1146/annurev-biochem-060409-092612 – volume: 9 start-page: 162 year: 2008 ident: 10.1016/j.ab.2013.08.020_b0015 article-title: Lipid signalling in disease publication-title: Nat. Rev. Mol. Cell. Biol. doi: 10.1038/nrm2335 – volume: 94 start-page: 2339 year: 1997 ident: 10.1016/j.ab.2013.08.020_b0050 article-title: Quantitative analysis of biological membrane lipids at the low picomole level by nano-electrospray ionization tandem mass spectrometry publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.94.6.2339 – volume: 2011 start-page: 748 year: 1811 ident: 10.1016/j.ab.2013.08.020_b0075 article-title: Quantitative analysis of glycerophospholipids by LC–MS: acquisition, data handling, and interpretation publication-title: Biochim. Biophys. Acta – volume: 375 start-page: 124 year: 2008 ident: 10.1016/j.ab.2013.08.020_b0080 article-title: Development of a reverse-phase liquid chromatography electrospray ionization mass spectrometry method for lipidomics, improving detection of phosphatidic acid and phosphatidylserine publication-title: Anal. Biochem. doi: 10.1016/j.ab.2007.12.027 – volume: 78 start-page: 6202 year: 2006 ident: 10.1016/j.ab.2013.08.020_b0055 article-title: Automated identification and quantification of glycerophospholipid molecular species by multiple precursor ion scanning publication-title: Anal. Chem. doi: 10.1021/ac060545x – volume: 55 start-page: 2579 year: 2006 ident: 10.1016/j.ab.2013.08.020_b0150 article-title: Altered adipose and plasma sphingolipid metabolism in obesity: a potential mechanism for cardiovascular and metabolic risk publication-title: Diabetes doi: 10.2337/db06-0330 – volume: 877 start-page: 2664 year: 2009 ident: 10.1016/j.ab.2013.08.020_b0070 article-title: High-throughput shotgun lipidomics by quadrupole time-of-flight mass spectrometry publication-title: J. Chromatogr. B doi: 10.1016/j.jchromb.2009.02.037 – volume: 282 start-page: 30845 year: 2007 ident: 10.1016/j.ab.2013.08.020_b0120 article-title: SLC1 and SLC4 encode partially redundant acyl-coenzyme A 1-acylglycerol-3-phosphate O-acyltransferases of budding yeast publication-title: J. Biol. Chem. doi: 10.1074/jbc.M702719200 – volume: 11 start-page: 593 year: 2010 ident: 10.1016/j.ab.2013.08.020_b0005 article-title: Lipidomics: coming to grips with lipid diversity publication-title: Nat. Rev. Mol. Cell. Biol. doi: 10.1038/nrm2934 – volume: 414 start-page: 266 year: 2011 ident: 10.1016/j.ab.2013.08.020_b0105 article-title: An ultraperformance liquid chromatography method for the normal-phase separation of lipids publication-title: Anal. Biochem. doi: 10.1016/j.ab.2011.03.009 – volume: 3 start-page: 433 year: 2010 ident: 10.1016/j.ab.2013.08.020_b0030 article-title: Advances in mass spectrometry for lipidomics publication-title: Annu. Rev. Anal. Chem. doi: 10.1146/annurev.anchem.111808.073705 – volume: 928 start-page: 127 year: 2001 ident: 10.1016/j.ab.2013.08.020_b0115 article-title: Assessment of the retention properties of poly(vinyl alcohol) stationary phase for lipid class profiling in liquid chromatography publication-title: J. Chromatogr. A doi: 10.1016/S0021-9673(01)01137-2 – volume: 77 start-page: 4 year: 2005 ident: 10.1016/j.ab.2013.08.020_b0040 article-title: Mediator lipidomics publication-title: Prostaglandins Other Lipid Mediat. doi: 10.1016/j.prostaglandins.2004.09.016 – volume: 108 start-page: 1903 year: 2011 ident: 10.1016/j.ab.2013.08.020_b0110 article-title: Membrane lipidome of an epithelial cell line publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.1019267108 – volume: 2011 start-page: 838 year: 1811 ident: 10.1016/j.ab.2013.08.020_b0045 article-title: Analysis of mammalian sphingolipids by liquid chromatography tandem mass spectrometry (LC–MS/MS) and tissue imaging mass spectrometry (TIMS) publication-title: Biochim. Biophys. Acta – volume: 49 start-page: 1113 year: 2008 ident: 10.1016/j.ab.2013.08.020_b0085 article-title: Quantitation of fatty acyl-coenzyme As in mammalian cells by liquid chromatography–electrospray ionization tandem mass spectrometry publication-title: J. Lipid Res. doi: 10.1194/jlr.D800001-JLR200 – volume: 2011 start-page: 763 year: 1811 ident: 10.1016/j.ab.2013.08.020_b0100 article-title: Liquid chromatographic–mass spectrometric analysis of phospholipids: chromatography, ionization, and quantification publication-title: Biochim. Biophys. Acta – volume: 302 start-page: 199 year: 2002 ident: 10.1016/j.ab.2013.08.020_b0140 article-title: Characterization and direct quantitation of ceramide molecular species from lipid extracts of biological samples by electrospray ionization tandem mass spectrometry publication-title: Anal. Biochem. doi: 10.1006/abio.2001.5536 – volume: 16 start-page: 1498 year: 2005 ident: 10.1016/j.ab.2013.08.020_b0130 article-title: Direct qualitative analysis of triacylglycerols by electrospray mass spectrometry using a linear ion trap publication-title: J. Am. Soc. Mass Spectrom. doi: 10.1016/j.jasms.2005.04.017 – volume: 78 start-page: 585 year: 2006 ident: 10.1016/j.ab.2013.08.020_b0135 article-title: Lipid profiling by multiple precursor and neutral loss scanning driven by the data-dependent acquisition publication-title: Anal. Chem. doi: 10.1021/ac051605m – volume: 77 start-page: 2166 year: 2005 ident: 10.1016/j.ab.2013.08.020_b0090 article-title: Automated quantitative analysis of complex lipidomes by liquid chromatography/mass spectrometry publication-title: Anal. Chem. doi: 10.1021/ac048489s – volume: 79 start-page: 4083 year: 2007 ident: 10.1016/j.ab.2013.08.020_b0060 article-title: Top-down lipidomic screens by multivariate analysis of high-resolution survey mass spectra publication-title: Anal. Chem. doi: 10.1021/ac062455y – volume: 24 start-page: 3159 year: 2005 ident: 10.1016/j.ab.2013.08.020_b0010 article-title: Cellular lipidomics publication-title: EMBO J. doi: 10.1038/sj.emboj.7600798 – volume: 31 start-page: 134 year: 2012 ident: 10.1016/j.ab.2013.08.020_b0020 article-title: Multi-dimensional mass spectrometry-based shotgun lipidomics and novel strategies for lipidomic analyses publication-title: Mass Spectrom. Rev. doi: 10.1002/mas.20342 – volume: 4 start-page: 594 year: 2005 ident: 10.1016/j.ab.2013.08.020_b0035 article-title: The emerging field of lipidomics publication-title: Nat. Rev. Drug Discov. doi: 10.1038/nrd1776 – volume: 47 start-page: 804 year: 2006 ident: 10.1016/j.ab.2013.08.020_b0095 article-title: LC–MS-based method for the qualitative and quantitative analysis of complex lipid mixtures publication-title: J. Lipid Res. doi: 10.1194/jlr.M500506-JLR200
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Snippet	Detailed analysis of lipid species can be challenging due to their structural diversity and wide concentration range in cells, tissues, and biofluids. To...
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SubjectTerms	3T3-L1 Cells - chemistry adipocytes Animals ceramides Ceramides - classification Ceramides - isolation & purification cerebellum Cerebellum - chemistry Chromatography, Liquid fatty acids Hydrophobic and Hydrophilic Interactions ionization Lipidomics liquid chromatography Mice Mice, Inbred C57BL Nanoelectrospray ionization Normal-phase LC Orbitrap mass spectrometry spectrometers Spectrometry, Mass, Electrospray Ionization surveys Tandem Mass Spectrometry tissues triacylglycerols Triglycerides - classification Triglycerides - isolation & purification
Title	Profiling of lipid species by normal-phase liquid chromatography, nanoelectrospray ionization, and ion trap–orbitrap mass spectrometry
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