Systematic mapping of functional enhancer-promoter connections with CRISPR interference

Gene expression in mammals is regulated by noncoding elements that can affect physiology and disease, yet the functions and target genes of most noncoding elements remain unknown. We present a high-throughput approach that uses clustered regularly interspaced short palindromic repeats (CRISPR) inter...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) Jg. 354; H. 6313; S. 769 - 773
Hauptverfasser:	Fulco, Charles P, Munschauer, Mathias, Anyoha, Rockwell, Munson, Glen, Grossman, Sharon R, Perez, Elizabeth M, Kane, Michael, Cleary, Brian, Lander, Eric S, Engreitz, Jesse M
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States 11.11.2016
Schlagworte:	Cell Proliferation - genetics Chromosome Mapping - methods Clustered Regularly Interspaced Short Palindromic Repeats CRISPR-Cas Systems Disease - genetics Enhancer Elements, Genetic - genetics Enhancer Elements, Genetic - physiology GATA1 Transcription Factor - genetics Gene Expression Regulation High-Throughput Nucleotide Sequencing - methods Humans K562 Cells Promoter Regions, Genetic - genetics Promoter Regions, Genetic - physiology Proto-Oncogene Proteins c-myc - genetics Real-Time Polymerase Chain Reaction
ISSN:	1095-9203
Online-Zugang:	Weitere Angaben
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