Automated 96-well format high throughput colony formation assay for siRNA library screen

The colony formation assay is the gold-standard technique to assess cell viability after treatment with cytotoxic reagents, ionizing radiation, and cytotoxic combinatorial treatments. This protocol describes a high-throughput automated and high-content imaging approach to screen siRNA molecular libr...

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Bibliographic Details
Published in:	STAR protocols Vol. 3; no. 2; p. 101355
Main Authors:	Hatch, Stephanie B., Prevo, Remko, Chan, Tiffany, Millar, Val, Cornelissen, Bart, Higgins, Geoff, Ebner, Daniel
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 17.06.2022 Elsevier
Subjects:	Cancer Cell Biology Cell culture Cell-based Assays Gene Library High Throughput Screening High-Throughput Screening Assays - methods Molecular Biology Protocol Radiation, Ionizing RNA, Small Interfering - genetics Single Cell Transfection Cell culture Molecular Biology Cell-based Assays High Throughput Screening Single Cell Cell Biology Cancer
ISSN:	2666-1667, 2666-1667
Online Access:	Get full text
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