Single-Cell Applications of Next-Generation Sequencing

The single cell is considered the basic unit of biology, and the pursuit of understanding how heterogeneous populations of cells can functionally coexist in tissues, organisms, microbial ecosystems, and even cancer, makes them the subject of intense study. Next-generation sequencing (NGS) of RNA and...

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Vydané v:Cold Spring Harbor perspectives in medicine Ročník 9; číslo 10
Hlavní autori: Anaparthy, Naishitha, Ho, Yu-Jui, Martelotto, Luciano, Hammell, Molly, Hicks, James
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: United States 01.10.2019
Predmet:
Animals
Biomedical Research - methods
High-Throughput Nucleotide Sequencing - methods
Humans
Neoplasms - genetics
Neurobiology
Single-Cell Analysis - methods
Transcriptome
ISSN:2157-1422, 2472-5412
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Popis
Shrnutí:The single cell is considered the basic unit of biology, and the pursuit of understanding how heterogeneous populations of cells can functionally coexist in tissues, organisms, microbial ecosystems, and even cancer, makes them the subject of intense study. Next-generation sequencing (NGS) of RNA and DNA has opened a new frontier of (single)-cell biology. Hundreds to millions of cells now can be assayed in parallel, providing the molecular profile of each cell in its milieu inexpensively and in a manner that can be analyzed mathematically. The goal of this article is to provide a high-level overview of single-cell sequencing for the nonexpert and show how its applications are influencing both basic and applied clinical studies in embryology, developmental genetics, and cancer.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ObjectType-Review-3
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ISSN:2157-1422
2472-5412
DOI:10.1101/cshperspect.a026898