Feasibility of flowcytometric quantitation of immune effector cell subsets in the sentinel lymph node of the breast after cryopreservation

The sentinel lymph node (SLN) is an emerging focus for immunological research in breast cancer. Cryopreservation of SLN single-cell suspensions allows for simultaneous phenotypic multi-parameter analyses and minimizes operator dependent variability. This is of particular importance for immunomonitor...

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Bibliographic Details
Published in:	Journal of immunological methods Vol. 375; no. 1-2; pp. 189 - 195
Main Authors:	van Pul, Kim M., Vuylsteke, Ronald J.C.L.M., Bril, Herman, Stockmann, Hein B.A.C., de Gruijl, Tanja D.
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 31.01.2012 Elsevier
Subjects:	Aged Biological and medical sciences Breast cancer breast neoplasms Breast Neoplasms - immunology Breast Neoplasms - metabolism Breast Neoplasms - pathology CD4 antigen CD4-CD8 Ratio - methods CD8 antigen CD8-Positive T-Lymphocytes - immunology CD8-Positive T-Lymphocytes - metabolism CD8-Positive T-Lymphocytes - pathology CD83 antigen CD86 antigen Clinical trials Cryopreservation Cryopreservation - methods Data processing Dendritic cells Dendritic Cells - immunology Dendritic Cells - metabolism Dendritic Cells - pathology Effector cells Female Flow cytometry Flow Cytometry - methods Forkhead Transcription Factors - immunology Forkhead Transcription Factors - metabolism Foxp3 protein freeze-thaw cycles Freeze-thawing Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunophenotype Lymph nodes Lymph Nodes - immunology Lymph Nodes - metabolism Lymph Nodes - pathology Lymphocytes T Middle Aged Molecular immunology patients Quantitation quantitative analysis Regulatory T cells Sentinel lymph node Sentinel Lymph Node Biopsy - methods T-Lymphocyte Subsets - immunology T-Lymphocyte Subsets - metabolism T-lymphocytes Techniques thawing variance cDC Dendritic cells Immunophenotype DMSO Sentinel lymph node CM FACS Quantitation Regulatory T cells MFI SLN Cryopreservation FCS DC Dendritic cell Lymph node T-Lymphocyte Cell subpopulation Quantitative analysis Immunological method
ISSN:	0022-1759, 1872-7905, 1872-7905
Online Access:	Get full text
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