Discovery of a novel Parvovirinae virus, porcine parvovirus 7, by metagenomic sequencing of porcine rectal swabs

Parvoviruses are a diverse group of viruses containing some of the smallest known species that are capable of infecting a wide range of animals. Metagenomic sequencing of pooled rectal swabs from adult pigs identified a 4103-bp contig consisting of two major open reading frames encoding proteins of...

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Bibliographic Details
Published in:Virus genes Vol. 52; no. 4; pp. 564 - 567
Main Authors: Palinski, Rachel M., Mitra, Namita, Hause, Ben M.
Format: Journal Article
Language:English
Published: New York Springer US 01.08.2016
Springer Nature B.V
Subjects:
adults
amino acids
Animals
Biomedical and Life Sciences
Biomedicine
Chiroptera
Genome, Viral - genetics
Medical Microbiology
metagenomics
Metagenomics - methods
open reading frames
Parvoviridae Infections - virology
Parvovirus, Porcine - genetics
Phylogeny
Plant Sciences
proteins
quantitative polymerase chain reaction
screening
Sequence Analysis, DNA - methods
swine
Swine - virology
Swine Diseases - virology
Ungulate protoparvovirus 1
United States
Virology
viruses
United States
Parvovirus
Swine
Parvoviridae
Fecal
Metagenomic sequencing
ISSN:0920-8569, 1572-994X
Online Access:Get full text
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Summary:Parvoviruses are a diverse group of viruses containing some of the smallest known species that are capable of infecting a wide range of animals. Metagenomic sequencing of pooled rectal swabs from adult pigs identified a 4103-bp contig consisting of two major open reading frames encoding proteins of 672 and 469 amino acids (aa) in length. BLASTP analysis of the 672-aa protein found 42.4 % identity to fruit bat ( Eidolon helvum ) parvovirus 2 (EhPV2) and 37.9 % to turkey parvovirus (TuPV) TP1-2012/HUN NS1 proteins. The 469-aa protein had no significant similarity to known proteins. Genetic and phylogenetic analyses suggest that PPV7, EhPV2, and TuPV represent a novel genus in the family Parvoviridae . Quantitative PCR screening of 182 porcine diagnostic samples found a total of 16 positives (8.6 %). Together, these data suggest that PPV7 is a highly divergent novel parvovirus prevalent within the US swine.
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ISSN:0920-8569
1572-994X
DOI:10.1007/s11262-016-1322-1